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Citations for Bio-Rad :
2901 - 2950 of 9412 citations for Recombinant Human LACRT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Total protein concentration of the supernatant was measured using the Bradford assay (Bradford Reagent Protein Assay Dye Reagent, Bio-Rad, cat. number 5000006). Unboiled protein samples were then mixed with 4x Laemmli sample buffer and 100 mM dithiothreitol (DTT) ...
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bioRxiv - Biochemistry 2020Quote: ... The B4GAT1 activity then was normalized against the amount of protein measured in the 10 µL of sample using the DC Protein Assay (Bio-Rad, Hercules, CA) with BSA as the standard ...
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bioRxiv - Immunology 2021Quote: ... the cell lysate in the sample buffer was loaded with equal amounts of protein into the wells of an Any kD mini-PROTEAN protein gels (Bio-Rad, Hercules, CA). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were centrifuged at 12,000g for 20 mins to remove cell debris and protein concentration in supernatants was determined using DC™ Protein Assay (Bio-Rad, #5000111). Twenty μg of proteins from L ...
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bioRxiv - Microbiology 2020Quote: ... The precipitated proteins were then dissolved in 6 M urea/50 mM ammonium bicarbonate buffer (pH 8) for protein quantitation using DC™ protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay, Bio-Rad, Richmond, CA) (Lowry et al ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the DC protein assay according to standard protocols provided by the kit manufacturer (Bio-Rad, Hercules, CA). Equal volumes of soluble protein supernatants were transferred to new tubes and subjected to in solution digestion ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yields were between 8 and 12 mg/L. Proteins were analyzed by SDS-PAGE (Supplementary Fig. 2a) using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). The gels were run at 200 V for 30 min.
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bioRxiv - Cell Biology 2022Quote: ... The total amount of protein isolated from the weighed tissues was determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). Hydroxyproline content data are expressed as μg of collagen per mg of total lung protein (μg/mg).
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred into clean tubes and the protein concentration was determined using the Bradford protein assay kit (Bio-Rad Laboratories, USA). The protein extracts (20 μg ...
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bioRxiv - Cell Biology 2021Quote: Total cell lysates were prepared in Laemmli SDS-sample buffer supplemented with Protease Inhibitor Cocktail (Nacalai Tesque, Kyoto, Japan) and protein concentrations were quantified by the DC protein assay (Bio-Rad, CA, USA). Protein concentrations were normalized if necessary and boiled for 5 min with 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2019Quote: ... of the protein was separated by SDS-PAGE in 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (cat. no. 4561084, Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μl of each sample was taken out separately for measuring protein concentration using Bradford protein assay reagent (BIO-RAD, Hercules, CA, USA). 5% of the lysates were boiled with 2 x laemmli buffer (BIO-RAD ...
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bioRxiv - Plant Biology 2019Quote: ... The protein concentration was quantified by Bradford assays and 10 μg of total protein for each sample was run on a 12.5% Criterion™ Tris-HCl protein gel (3450014, Bio-Rad, Hercules, CA). Following electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: 15 μg of total protein extract from purified proteins were separated by native electrophoresis using a Tetra cell (Bio-Rad; Hercules, CA, USA), in 12% polyacrylamide separation gel and a 4% polyacrylamide stacking gel in non-denaturing conditions ...
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bioRxiv - Plant Biology 2021Quote: ... The protein concentration of each of the five biological replicates was estimated using a Bradford protein assay kit (Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration in supernatant will be determined by Bradford assay using the Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of total protein are separated on 8∼12% SDS–PAGE gels ...
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bioRxiv - Cell Biology 2020Quote: ... The molecular weights of protein bands were estimated using the Precision Plus Protein™ Dual Color Standards (#161-0374; BIO-RAD, Hercules, CA).
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was used for various assays and the protein content of the homogenate was measured using DC protein assay kit (Bio-Rad, Hercules, CA). Lactase activity was assessed using a Lactase Assay Kit (Cat# E-BC-K131-S ...
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bioRxiv - Genetics 2021Quote: ... Samples were then centrifuged at 4°C for 5 min at 20,000 g and protein concentrations in the supernatants were measured using the Bradford assay (Bio-Rad Protein Assay Dye). Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatants were transferred to new tubes and protein concentrations were determined using the Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA). Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Total AGT concentration in each sample was normalized by total protein concentrations measured using a DC™ protein assay kit (Bio-Rad 5000112).
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bioRxiv - Genetics 2019Quote: ... We measured total protein concentrations of cell lysates with the BCA protein assay kit (Pierce, Cat#23225) and diluted 40 μg of each protein with XT sample buffer (Bio-Rad, Cat#1610791) and XT reducing agent (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Amylose resin samples were transferred to columns and protein-bound beads were washed with lysis buffer until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Maltose-binding protein fusions were eluted with 12 ml elution buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... beads were removed via a magnetic rack and proteins loaded on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were added to each lane and run on a 12 % SDS-PAGE gel at 18 V/cm to separate the total protein and transfer protein into PVDF membranes using a semi-dry transfer device (Bio-Rad, CA, USA). The membranes were collected and blocked for 1 hour at room temperature with 5% bovine serum albumin (BSA) ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ug of protein was loaded onto precast gel (4561095, 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Bio-Rad, USA) and resolved at 200 volts for 30-35 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The clear supernatants were then transferred into clean tubes and total protein concentration measured using a DC protein assay (Bio-Rad, Hercules, CA). To prepare for ELISA ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration in samples was determined with the Bradford assay using a Protein Assay Dye Reagent (#500-0006, Bio-Rad, Richmond, CA) with optical density reading at 595 nm (OD595) ...
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bioRxiv - Neuroscience 2022Quote: Protein sample was loaded into each well of a 4-20% Mini-PROTEAN TGX Precast protein 15-well gels (Bio-Rad, cat#4561096). Protein was transferred using transfer buffer (20% MeOH in TBS ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed using RIPA buffer and total protein concentration was quantified using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006). Twenty micrograms of protein were incubated in Laemmli sample buffer at 95°C for 5 min and subjected to electrophoresis on a 3-15% gradient polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondria-containing pellet was resuspended in THE buffer without BSA and the protein concentration measured using the Bradford Protein Assay (Bio-Rad, Munich, Germany). The protein concentration was adjusted to 10 µg/µl with THE buffer.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A total of 20 μg of protein was separated using a 4%–15% Criterion™ XT tris-glycine protein gel (BioRad, United States) and then transferred to nitrocellulose membrane (BioRad ...
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bioRxiv - Immunology 2023Quote: ... and equal protein amounts were separated using ready-to-use 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Biorad, 4568096) and transferred onto 0.2 μm pore nitrocellulose membranes (PALL Life Sciences 66485).
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... and protein-bound beads were washed with 60ml lysis buffer until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Biochemistry 2023Quote: Final carboxysome samples and as well as the lysate were analyzed for presence of CsoSCA or sfGFP-fusion protein by SDS-page (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad)) ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a new microcentrifuge tube and protein concentration was determined by using a DC protein assay kit (BioRad, Cat# 500-0116). Cell lysates (500-1000 μg ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μg of each protein sample was separated by electrophoresis and proteins were then transferred onto a Trans-Blot® Turbo™ PVDF Membrane (Biorad 10026933) at 2.5 Å for 3 minutes using Trans-Blot® Turbo™ Transfer System (Biorad 1704150) ...
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bioRxiv - Plant Biology 2023Quote: ... Fifty micrograms of protein for each treatment was loaded onto a (4-20)% acrylamide SDS-PAGE denaturing protein gel (Bio-Rad, Hercules, USA) and separated at 4°C at 150 constant volts ...
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bioRxiv - Physiology 2023Quote: ... protein content in the soluble fraction of homogenates was measured using a RC-PC protein assay kit (DC Protein Assay Kit, Bio-Rad, Hercules, CA), and then dissolved in Laemmli buffer.
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg protein were subjected to electrophoretic run using a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories), transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2023Quote: ... 20-25ug of protein lysate was loaded per well and separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (Bio-Rad). Proteins were transferred onto an activated PVDF membrane using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 30μg of total protein lysate of each sample was separated by electrophoresis in 12% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) and transferred using a Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysates were cleared by centrifugation at 10,000 g for 5 minutes and protein concentration was determined using the DC Protein Assay (Bio-Rad Laboratories, Watford, UK) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and 8ug of total protein from each lysate was separated on a 4-15% SDS-polyacrylamide gel (Mini-PROTEAN® TGX™ Precast Protein Gels (BIO-RAD). After the proteins were transferred to nitrocellulose membrane (BIO-RAD) ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of protein per sample were loaded into each lane of 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were transferred to nitrocellulose membrane using semidry Trans- Blot® Turbo™ Transfer system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...