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Citations for Bio-Rad :
2851 - 2900 of 3951 citations for 6 amino m xylene 4 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h and then incubated with primary overnight and secondary antibodies for 2 h ...
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bioRxiv - Developmental Biology 2019Quote: ... monomeric ATP-bound 2 μM rabbit muscle actin was treated overnight at 4°C with analytical grade anion exchange resin (BioRad), hexokinase (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h in nonfat-milk ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged at 30,000 x g for 30 min at 4°C before being loaded into a gravity flow column (Bio-Rad) and purified by Ni-NTA resin (UBP Bio ...
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bioRxiv - Genetics 2021Quote: ... rotated at 4°C for 2h and then poured into a Econo-Column® Chromatography column (Bio-Rad, Hercules, CA). After extensive washing first with 80 mL of 20 mM Tris HCl [pH8@4°C] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were run on a 4–20% SDS-PAGE and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories) using a semidry blotting apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... The testicular extract was run on a SDS-PAGE 4-15% mini-protean TGX precast gel (Bio-Rad, 456-1084) and transferred to a nitrocellulose membrane ...
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bioRxiv - Immunology 2021Quote: ... The material bound on the beads was eluted in Laemmli buffer and the proteins were separated by SDS-PAGE using Criterion 4-15% acrylamide gels (BioRad) in Tris-Glycine-SDS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and eIF2B4 loci were amplified with the primer pairs detailed in Table 4 and run on a 1% agarose gel and imaged using a ChemiDoc XRS+ imaging system (Biorad). The expected WT fragment length for the eIF2B1 ...
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bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was centrifuged at 30,000 x g for 30 min at 4 °C before being loaded into a gravity flow column (Bio-Rad) and purified by Ni-NTA resin (UBP Bio ...
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bioRxiv - Bioengineering 2020Quote: ... NJ) overnight at 4°C and visualized using the Bio-Rad ChemiDoc MP Imaging System (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Biochemistry 2021Quote: ... respectively 45 μL and 10 μL of proteins were loaded for each condition and separated on gradient 4-12% polyacrylamide TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant < 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL BHI medium + 0.5 mM saccharose was added and the cells were incubated at 30 °C for 4 h and plated on BHI agar plates containing 10 μg/mL erythromycin ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2020Quote: Cross-linked samples of purified ROS-GC1 fragment (aa 814-1110) were analyzed by SDS-PAGE using gradient gels (4-20%, Mini-PROTEAN TGX gels, Biorad). For in-gel digestion ...
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bioRxiv - Biophysics 2020Quote: ... samples were washed for two minutes with degassed polyacrylamide (PA) solution consisting of 4% (vol/vol) 19:1 acrylamide/bis-acrylamide (161010144, BioRad), 60 mM Tris-HCl pH 8 (AM9856 ...
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bioRxiv - Cancer Biology 2020Quote: Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 12 μg of total protein were loaded onto a 4-20% mini Protean TGX gel (Bio-Rad, Hercules, CA) and blots were evaluated as described previously
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded in a 10-well 4-20% Mini-PROTEAN TGX Precast protein gel (BIO-RAD, Catalog #4561094). The gel was run at 150V for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and then resuspended in 60 μl lysis buffer and 20 μl 4 x Laemmli Sample Buffer (Bio-RAD, 161-0747), heat shocked in a thermomixer (Eppendorf ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated by protein electrophoresis at 150 V for 1h using 4-20% Mini-Protean TGX pre-cast gels (BioRad) and transferred by semi-dry technic onto Amersham Hybond-P PVDF membranes (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were spun down and loaded onto a 4-20% (w/v) polyacrylamide precast mini-PROTEAN TGX gel (Bio-Rad) for subsequent gel-electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted in SDS page loading buffer and separated on 4-15% Mini-PROTEAN TGXTM Precast Protein Gels (BioRad). Samples were processed for immunoblotting or fixed and stained for mass spectrometry using Imperial protein stain (Pierce ...
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bioRxiv - Physiology 2021Quote: ... or chylomicron fractions were mixed with 4x laemmli buffer and loaded onto 4%–15% TGX Gels (Bio-Rad, Hercules, CA), transferred to hybond PVDF membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified and the same amount of protein was loaded to the wells in a 4-15% SDS-PAGE gel (BioRad). Protein was transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: SDS-PAGE and western blotting were performed using 4-20 % Mini-PROTEAN TGX Stain-free Precast Gels (Bio-Rad, 4568094) and Trans-Blot Turbo 0.2 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Protein sample was incubated at 98°C for 5 min and ran on Bio-Rad TGX Stain-Free Precast Gel (4-15%) in Tris/Glycine/SDS running buffer (BioRad). Then the gel was transferred onto a 0.45 μm PVDF membrane (Thermofisher) ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of protein was run per sample on 4–15% Mini-Protean TGX stain-free precast gels (Bio-Rad) at 150 V ...
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bioRxiv - Microbiology 2022Quote: ... total protein lysates of T.brucei BSF were separated by SDS-PAGE (4-20% Mini PROTEAN TGX stain-free precast gradient gels, Bio-Rad) and blotted on PVDF filters (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... P14 and P18 retinas were measured by fluorescent Western blot analysis [4-15% or 7.5% Mini-PROTEAN TGX gels (Bio-Rad)] and quantitation was performed using the LICOR (LICOR ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were separated by precast 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad Laboratories 5678085), transferred to a nitrocellulose membrane using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad Laboratories 1704159EDU ...
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The whole cell lysate and crude cytoskeletons were resolved on 4-20% Mini-PROTEAN tris-glycine polyacrylamide gels (Bio-Rad), and Western blots were stained with 1:5000 diluted anti-beta-actin mouse monoclonal antibody (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed with IP lysis buffer three time and boiled in 25 ul of 2X SDS-loading buffer for 5 minutes and loaded into 4-15% polyacrylamide gels (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Cell Biology 2022Quote: ... 15 ug of mitochondrial proteins or 25 ug of total cellular proteins were separated by gel electrophoresis on 4-20% gradient TGX gels and transferred onto PVDF or nitrocellulose membranes using Trans-Blot Turbo Transfer system (BioRad). Mitochondrial electron transport chain proteins were detected using Total OXPHOS Rodent WB Antibody Cocktail (ab110413) ...
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bioRxiv - Biochemistry 2020Quote: ... 750 ng of wtXLF and each tandem dimer construct were loaded and run on a 4-15% Mini-PROTEAN TGX Precast Protein Gel (BioRad). tdXLF constructs where the KBM of subunit 1 was replaced with additional linker sequence (lane 4 tdXLFΔKBM/WT and lane 6 tdXLFΔKBM/ΔKBM ...
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bioRxiv - Neuroscience 2021Quote: ... Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad) and transferred on to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast concentration was adjusted to 4 × 108 cells/ml and added to the same amount of 1.2% low melting agarose gel (Bio-Rad) solution ...