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Citations for Bio-Rad :
2851 - 2900 of 6641 citations for 6 1 FLUOROETHYL 4 1H PYRIMIDINONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 10% of 2 mM EGTA eluates were concentrated and separated on 4%– 20% gradient SDS-polyacrylamide Tris-glycine gels (Biorad). Total protein content was visualized by silver staining ...
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to myosin II was estimated by a gel-shift assay (4–15% gradient gel, Biorad) and determined as >99% for both S1 and lever-arm-less S1 (Supplementary Fig ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were reduced in LDS with beta-mercaptoethanol and then polyacrylamide gel electrophoresis was performed on 4-20% Protean Mini TGX gels (Biorad) and transferred to Immobilon PVDF membranes for 15 minutes using mini TGX settings on the Trans-Blot-Turbo system (Biorad) ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted directly in sample buffer and samples were separated on a 4-12% Criterion Bis-Tris gel (BioRad). Proteins were transferred to PVDF membrane (Immobilon-FL ...
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Nucleosome fractions containing 200 ng DNA were applied for SDS-PAGE with 4-20 % gradient SDS-PAGE gel (Bio-rad). Proteins were transferred to the nitrocellulose membrane (GE ...
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bioRxiv - Genetics 2020Quote: Equal volumes of lysate from each fraction were separated by SDS-PAGE using 4-20% TGX Stain-Free mini gels (BioRad). Protein was transferred to PVDF membrane using the TransBlot Turbo system (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ... Western blot assays were performed after resolving the samples on a 4-15 % Tris-HCl gradient gel (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2020Quote: ... Specific volumes of samples (described previously) were loaded on a 4−20 % Mini-PROTEAN-TGX gel (BioRad, Hercules, CA, USA) and run at 180 V for 45 min ...
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bioRxiv - Cell Biology 2019Quote: ... SDS dye was added to a 1X concentration and lysates (equal µg of protein) were loaded onto 4-20% Tris-glycine gels (BioRad). Gels were transferred to nitrocellulose ...
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bioRxiv - Physiology 2020Quote: ... Ten micrograms of protein were subjected to SDS-PAGE on 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad) and transferred to polyvinylidene diflouride (PVDF ...
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bioRxiv - Physiology 2020Quote: ... equal amounts of protein (20 ug) were loaded in 4-12% - Criterion XT Bis-Tris protein gels (Bio-Rad, CA) and transferred to nitrocellulose membranes ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Images were taken after 3 to 4 days (unless indicated otherwise) using the ChemiDoc™ Touch Imaging System (Bio-Rad). Agar plates contained the vital dye Phloxine B at a final concentration of 8 µg/mL.
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Physiology 2021Quote: ... we ran the purified Hb fractions on a mini-protean precast 4-20% SDS PAGE gel (Bio-Rad, Hercules, CA), which we then stained with Coomassie brilliant blue-G ...
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bioRxiv - Physiology 2021Quote: ... were handpicked to match size of islets across the samples and loaded into the polycarbonate perifusion chamber between two layers of polyacrylamide-microbead slurry (Bio-Gel P-4, BioRad) by the same experienced operator ...
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bioRxiv - Developmental Biology 2019Quote: ... NuPage 4-12% Bis-Tris protein gels beofre transferring into PVDF membranes using the Trans-Blot Turbo transfer system (Biorad). Prior to blocking and antibody incubation ...
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 20 μL of the total crude cell lysate from each sample was loaded onto a 4–20% gradient Tris-HCl Criterion SDS-PAGE (BioRad). After transfer to polyvinylidene fluoride membrane ...
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bioRxiv - Genomics 2019Quote: ... at 4 °C and transferred to a nitrocellulose membrane (0.45 μM) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current of 0.12 A for 1 □ hour ...
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bioRxiv - Cell Biology 2019Quote: ... a total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
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bioRxiv - Cell Biology 2019Quote: ... Immunoblotting was performed by standard methods using 4-15% Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad). Ponceau S staining of membranes was routinely used to assess equal sample loading and transfer efficiency ...
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bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
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bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed directly in sample buffer and protein extracts were separated on a 4-20% polyacrylamide gel (Bio-Rad). Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53 ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: Eluted samples were resolved by SDS-PAGE using 4-20% gradient gels and then transferred to the nitrocellulose membrane using the Trans-Blot Turbo transfer system (Biorad). Following the blocking for 1 hour in blocking buffer (5% milk in TBST) ...
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bioRxiv - Biochemistry 2021Quote: ... two probes (250 nM final each) (Supplementary Table 4) and template DNA were combined with ddPCR Supermix for Probes (No dUTP) (BioRad) in a final 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg of protein was loaded on 4-20% Mini-PROTEAN TGS Precast Protein Gels (Cat. No. 4561094, Bio-Rad) from each condition and ran at 100 V for 1 hour followed by transferring the protein on a PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated according to size by SDS-PAGE in a Mini-Protean TGX stain-free 4-20% gradient gel (BioRad) and thereafter transferred onto a PVDF membrane ...
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bioRxiv - Physiology 2021Quote: ... and proteins were stripped from beads by incubation in gel loading buffer with beta-mercaptoethanol and loaded to 4-15 % gels (Criterion; BioRad) for SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant ~ 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL warm BHI medium was added and the cells were incubated at 30 °C for 6 h under shaking at 200 rpm and plated on BHI agar plates with 10 μg/mL erythromycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 20 mg of total protein were loaded for electrophoresis into 4–20% sodium dodecyl sulfate polyacrylamide gels (Bio-Rad). Separated proteins were transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Proteins were separated by 4-15% SDS-PAGE gel (Bis-Tris Criterion™ TGX™ pre-cast gels, Bio-Rad) and transferred to PVDF membranes (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad). The co-immunoprecipitation was performed using GFP trap® beads (Chromotek ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoblotting was performed with 4-15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad, Hercules, CA). Membranes were blocked with 5% milk in TBS with 0.1% Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples from total or immunopurified ribosomes or aliquots from TCA precipitated sucrose gradient fractions were run in 1X Laemmli buffer on 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad) in 1X Tris/Glycine/SDS buffer (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cell lysates and 5 μl of purified viruses were diluted in Laemmli buffer and loaded on a pre-casted Criterion XT 4-12% gradient gel (Biorad). Gels were transfered to PVDF membrane using a wet-electroblotting chamber system (Biorad ...
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bioRxiv - Neuroscience 2021Quote: Soluble protein cellular lysates (20μg) were analysed by 4-15% gradient SDS-PAGE and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved using 4-12% BisTris-PAGE and transferred to a PVDF membranes (Bio-Rad catalogue number 162-0177) using a Gel Transfer Cell (BioRad catalogue number 1703930) ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... 40 μg of cell lysates was separated by 4-20% Tris-glycine MINI-PROTEAN TGX gels (Bio-Rad, 456-1094) and transferred to PVD membranes using Trans-Blot Turbo Transfer Pack (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: SDS-page gel electrophoresis analysis (12.5% running gel, 4% stacking gel stained with Coomassie brilliant blue R-250 Bio-Rad) was carried out for checking the yield of PsbS at every step of overexpression ...
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 95°C for 5 min and the transcription products were resolved on denaturing 4% polyacrylamide gel and visualized using a Personal Molecular Imager (BioRad).