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Citations for Bio-Rad :
2851 - 2900 of 4939 citations for 4 Phenyl 3 buten 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... using the primers listed in Supplementary Table 2 using SsoFast EvaGreen Supermix (Bio-Rad). The expression of the respective gene of interest was normalized to the samples’ respective GAPDH expression using the ΔCt or ΔΔCt method as indicated in the figures ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and loaded onto a 2 ml CHT2-I hydroxyapatite column (Bio-Rad) equilibrated in 20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and immunoprecipitates on beads were resuspended in 2× Laemmli buffer (Bio-Rad) and subjected to SDS-PAGE and western blot analysis.
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bioRxiv - Bioengineering 2021Quote: ... 2% milk solution was prepared by dissolving nonfat dry milk powder (Bio-rad, UK) in distilled water and this solution was mixed with equal volume of 2% ...
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bioRxiv - Neuroscience 2019Quote: ... Bound proteins were eluted in 2 × Laemmli sample buffer (Bio-Rad, Cat# 161-0737) containing 5% 2-mercaptoethanol by heating at 95 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... sections were blocked with 2% milk powder (blotting grade; Bio-Rad Laboratories, Hercules, USA) in PBS for 30 minutes ...
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bioRxiv - Genomics 2021Quote: These reactions were undertaken on a DNA Engine Tetrad 2 Thermal Cycler (Bio-Rad), with MyTaq HS DNA polymerase ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted proteins were further purified using the BioRad ReadyPrep 2-D Cleanup Kit (BioRad), approximately 60-70 μg of protein was loaded onto 7 cm pH 3-10 NL and pH 4-7 IPG strips (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... Luminescence intensities mm-2 were evaluated using the Image Lab software (BioRad, Feldkirchen, Germany).
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bioRxiv - Microbiology 2022Quote: ... The cells:DNA mix was transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082) and electroporated at 2500 kV ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 2 h on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Immunology 2022Quote: ... and then blotted for 2 hours onto 0.45 μm supported nitrocellulose membranes (Bio-Rad). Membranes were blocked for one hour using 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated for 2 minutes with Clarity Western ECL Blotting Substrate (Bio-Rad) and proteins visualized on ChemiDoc (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... This mixture was transferred to a 2 mm ice cold electroporation cuvette (Bio-Rad). Transformation of WT P ...
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bioRxiv - Microbiology 2024Quote: ... The assays were incubated for 2 hours at 37 °C in a thermocycler (Biorad). Samples were then filtered through 3 kDa filter plates and derivatized with 4,5-dimethoxy-1,2-phenylenediamine hydrochloride (DMB) ...
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bioRxiv - Microbiology 2024Quote: ... the beads and total lysate were resuspended in 2× Laemmli sample buffer (Bio-Rad) supplemented with 5% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The purity of 12mer arrays was confirmed using APAGE (2% acrylamide, 1% Biorad agarose) gels stained with SyBr Gold (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins bound to Dynabeads were eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... then 2 ul of normalized RNA were mixed with iTaq Universal SYBR (Bio-Rad) and primers for c-Myc (F ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... and re-suspended in 2x Laemmli sample buffer containing 2% SDS (Biorad, catalog #: 1610737). Protein concentration was measured by MicroBCA assay (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Afterwards the samples were incubated with Bio-Beads SM-2 sorbent (Bio-Rad Laboratories) overnight on the rolling bank at 4°C to remove the detergent (Rigaud et al ...
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bioRxiv - Biochemistry 2023Quote: ... detergent was removed by overnight incubation with activated Bio-Beads SM-2 (Bio-Rad). For purification of PhuR-containing nanodiscs ...
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bioRxiv - Immunology 2023Quote: ... 2× ddPCR supermix for probes (no dUTP) final concentration 1× (Bio-Rad Laboratories, USA), a 20× target primer/FAM-labeled probe mix ...
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bioRxiv - Plant Biology 2023Quote: ... Each reaction contained 5 μL of 2 x iQ SYBR Green supermix (Bio-Rad), 2 μL of diluted cDNA (10 times dilution for shoots and roots ...
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bioRxiv - Immunology 2024Quote: ... Excess APTS was removed using Bio-Gel P-2 size exclusion resin (Bio-Rad) (antigen specific glycans ...
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bioRxiv - Synthetic Biology 2024Quote: ... mixed with the ligation mixtures and transferred to chilled 2 mm electroporation cuvettes (BioRad). Cells were transformed using a Gene Pulser II electoporator (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
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bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
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bioRxiv - Bioengineering 2019Quote: Proliferation of MSCs was evaluated by determining the total cell count per well (n=3 wells/treatment/time-point) using a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: Home-made gels: The home-made Laemmli resolving gel (10 ml) contains 3 ml of 40% Acrylamide/Bis (cat#: 1610148, Bio-Rad), 2.5 ml of 1.5M Tris-HCl pH 8.8 (cat# ...
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bioRxiv - Zoology 2019Quote: ... 0.5 ml of the heptane phase were mixed with ethanol in a 1:3 ratio (v/v) and the absorbance at 233 nm was measured on a SmartSpec Plus spectrophotometer (Bio-Rad) with extraction blanks used as references ...
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bioRxiv - Cell Biology 2019Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Thermal cycler, BIO-RAD), followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad, Hercules, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with gl3 specific primers (Supplemental Table 3) and iQ™ SYBR® Green Supermix (BioRad, USA) and conducted on a BioRad CFX with 96-well reaction blocks under the following PCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...