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Citations for Bio-Rad :
2801 - 2850 of 9210 citations for Borrelia burgdorferi sensu stricto B31 ErpN OspE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a new microcentrifuge tube and protein concentration was determined by using a DC protein assay kit (BioRad, Cat# 500-0116). Cell lysates (500-1000 μg ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μg of each protein sample was separated by electrophoresis and proteins were then transferred onto a Trans-Blot® Turbo™ PVDF Membrane (Biorad 10026933) at 2.5 Å for 3 minutes using Trans-Blot® Turbo™ Transfer System (Biorad 1704150) ...
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bioRxiv - Plant Biology 2023Quote: ... Fifty micrograms of protein for each treatment was loaded onto a (4-20)% acrylamide SDS-PAGE denaturing protein gel (Bio-Rad, Hercules, USA) and separated at 4°C at 150 constant volts ...
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bioRxiv - Physiology 2023Quote: ... protein content in the soluble fraction of homogenates was measured using a RC-PC protein assay kit (DC Protein Assay Kit, Bio-Rad, Hercules, CA), and then dissolved in Laemmli buffer.
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg protein were subjected to electrophoretic run using a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories), transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2023Quote: ... 20-25ug of protein lysate was loaded per well and separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (Bio-Rad). Proteins were transferred onto an activated PVDF membrane using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 30μg of total protein lysate of each sample was separated by electrophoresis in 12% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) and transferred using a Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysates were cleared by centrifugation at 10,000 g for 5 minutes and protein concentration was determined using the DC Protein Assay (Bio-Rad Laboratories, Watford, UK) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and 8ug of total protein from each lysate was separated on a 4-15% SDS-polyacrylamide gel (Mini-PROTEAN® TGX™ Precast Protein Gels (BIO-RAD). After the proteins were transferred to nitrocellulose membrane (BIO-RAD) ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of protein per sample were loaded into each lane of 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were transferred to nitrocellulose membrane using semidry Trans- Blot® Turbo™ Transfer system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 ug of protein was loaded in each lane of a 4– 15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad 4561083). After SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of total protein mite extracts was determined using the Quick Start Bradford Protein Assay (Quick start Bradford 1x dye reagent, Bio-Rad, Cat# 500-0205), with Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Bradford Protein assay method was used to determine the protein concentration using bovine serum albumin (BSA) as the standard (Bio-Rad catalog 500-0006). 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 μg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Cell Biology 2021Quote: ... Sample proteins were extracted using RIPA buffer and the concentration protein in the supernatant was determined by the Bradford method (Bio-Rad Lab, California, USA). Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 µg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Molecular Biology 2019Quote: ... unbound and eluted proteins were precipitated by TCA extraction before running on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad #4561096) and western blotting was performed as described.
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bioRxiv - Biochemistry 2019Quote: ... The microsomal fraction was aliquoted and stored at −80 °C after protein quantification with the Quick Start Bradford Protein Assay Kit (Bio-Rad Laboratories, Munich, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Genetics 2020Quote: ... the soluble fraction was transferred to a new tube and total protein concentration was determined by DC protein assay (Bio-Rad, Hercules, CA, USA). Following quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... The fluorescence intensity was calculated relative to protein concentration in each sample as assessed by the DC™ Protein Assay (BioRad, Hercules, CA, USA) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... Elution fractions of protein purifications were analyzed on 12 % SDS-PAGE gels and the protein concentrations were determined by Bradford (Bio-Rad Laboratories, Hercules, California) or Qubit protein assay (Thermo Fisher Sceintific ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... separated by electrophoresis and transferred to nitrocellulose membrane accordingly.1 The molecular mass of protein was determined by comparison with the migration pattern of Precision Plus Protein™ Prestained Standards Dual Color (Biorad Laboratories, CA, USA). The primary antibodies used were anti-pAKT (S473) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein levels were normalized to the unphosphorylated version of the protein or the total protein content per lane as assessed via stain-free TGX gel technology (Bio-Rad, Hercules, CA, USA) [49] ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Neuroscience 2024Quote: Approximately 15 μg of synaptosome protein was separated on a precast gradient gel (4-15% Mini-PROTEAN® TGX™ Precast Protein Gels, 4561086, BioRad, United Kingdom) and transferred to nitrocellulose membrane (Amersham™ ProtranÒ Western Blotting Membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... with an All-Blue precision plus protein ladder (Bio-Rad) at 70 V for approximately 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was resolved on SDS polyacrylamide gels (Bio-Rad) with DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were imaged on Chemidoc MP (Bio-Rad, Hercules, CA) with ECL reagent.
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bioRxiv - Cancer Biology 2021Quote: ... and separated on 4-15% Protein Gels (BioRad, Cat# 4568084). SPOP protein was probed by using in-house made rabbit anti-SPOP (1:1000) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were determined using the Bradford assay (Bio-Rad). Lysates were mixed with 4X Laemmli buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were quantified by Bradford assay (Bio-Rad, Hercules, CA). Equal amounts of proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations in all samples were determined by Bio-Rad DC protein assay as a routine ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Bio-Rad Laboratories) using the TurboBlot(tm ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with the enhanced chemiluminescence substrate ECL (Biorad) and imaged using the ChemiDoc XRS Biorad Imager and Image Lab Software.
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bioRxiv - Cell Biology 2020Quote: Protein bands intensities were quantified with Image Lab 6 (BioRad). Equal rectangles were drawn around the total protein gel lane and the band of interest ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then transferred onto nitrocellulose membrane (BioRad, Germany) using a vacuum Blotter (Model 785 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred to a nitrocellulose transfer membrane (Bio-Rad). Membranes were blocked ...