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Citations for Bio-Rad :
2801 - 2850 of 4305 citations for 6 4 Methoxybenzylcarbamoyl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: Homogenized cell lysates were processed via SDS-PAGE (4-20% BioRad Mini-PROTEAN® TGX™ precast gels), transferred to a membrane (Millipore Immobilon-FL PVDF) ...
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bioRxiv - Physiology 2024Quote: ... 20 ug protein was separated in 4-20% Mini-Protean TGX Precast Gels (Bio-Rad, California, US, #4561094) and blotted using Bio-Rad Trans-Blot Turbo device (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mixed by pipetting and loaded onto 4-15% gradient Mini-PROTEAN TGX gels (Bio-Rad, 4561086). Following gel electrophoresis in Tris-Glycine-SDS running buffer (Boston BioProducts ...
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bioRxiv - Immunology 2024Quote: ... Twenty microliters of denatured samples were loaded on a PROTEAN TGX 4-20% stain-free precast gel (Biorad) in 1× Tris-Glycine-SDS running buffer (Alfa Aesar) ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatants were run in a 4-15 % mini-protean TGX precast gel (Bio-Rad, cat. no. 4561084) next to PageRulerTM prestained protein ladder (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... and protein extract was resolved by electrophoresis on a precast 4-20% tris-glycine polyacrylamide gels (Bio-Rad), transferred to polyvinylidene difluoride membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were loaded on SDS-PAGE Criterion precast 4-15% gradient gel using Criterion System (Bio-Rad). Resolved proteins were transferred onto PVDF membrane by using Criterion Blotter system (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: Samples were electrophoresed on BioRad mini-PROTEAN TGX Stain-Free 4-20%,15-well gradient gels (BioRad, USA), and transferred toPVDF membranes (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Then 50 μg of protein lysate was electrophoresed on criterion TGX 4-15% precast gel (Bio-Rad Laboratories) and transferred onto PVDF membrane (Millipore) ...
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bioRxiv - Physiology 2024Quote: ... run on denaturing SDS-PAGE gels (4%-20%, Mini-PROTEAN TGX™ stain-free precast gels, Bio-Rad), and transferred to a PVDF membrane (Trans-Blot Turbo™ PVDF transfer system ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were shortly vortexed and loaded onto 4-15% Criterion TGX Stain-Free gel (BIO-RAD, 5678085). After running the gel (90V ...
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bioRxiv - Biochemistry 2024Quote: ... The SEC fractions were analyzed by SDS-PAGE using 4-20% precast protein gels (BioRad, Hercules, CA, USA), pre-stained protein ladder (#26619 ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µg of proteins were loaded per well on a precast 4-20%TGX gel (Bio-Rad, #4561094), and run in TGS buffer at 200 V for 45 min.
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bioRxiv - Biochemistry 2024Quote: ... All samples were boiled and separated on SDS-PAGE using a pre-cast gradient gel (4-15%, BioRad). Proteins were transferred to nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis on 4–20% Mini- PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) and transferred to Immobilon®-P PVDF Membrane (EMD Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... The worm lysates were separated on 4–15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS PAGE) (Bio-Rad). The proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20μg of protein lysates were run on 4%–20% Mini- PROTEAN TGX Precast Protein Gels (#4561096; Biorad) and transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... SDS page was run on 4-15% gradient protein gels using the Criterion Electrophoresis Cell (Bio-Rad, 1656001) and transferred to a PVDF membrane using the Trans-Blot semi-dry transfer system (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... a total of 6 × 107 cells was incubated in staining buffer with mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:50) at 4°C for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
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bioRxiv - Bioengineering 2019Quote: Proliferation of MSCs was evaluated by determining the total cell count per well (n=3 wells/treatment/time-point) using a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: Home-made gels: The home-made Laemmli resolving gel (10 ml) contains 3 ml of 40% Acrylamide/Bis (cat#: 1610148, Bio-Rad), 2.5 ml of 1.5M Tris-HCl pH 8.8 (cat# ...
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bioRxiv - Zoology 2019Quote: ... 0.5 ml of the heptane phase were mixed with ethanol in a 1:3 ratio (v/v) and the absorbance at 233 nm was measured on a SmartSpec Plus spectrophotometer (Bio-Rad) with extraction blanks used as references ...
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bioRxiv - Cell Biology 2019Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Thermal cycler, BIO-RAD), followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad, Hercules, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with gl3 specific primers (Supplemental Table 3) and iQ™ SYBR® Green Supermix (BioRad, USA) and conducted on a BioRad CFX with 96-well reaction blocks under the following PCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before detection using Clarity Western ECL Substrate (Bio-Rad 170-5061) and imaging on a ChemiDoc MP (Bio-Rad 17001402).
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bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Specific primers against the genes of interest (Table 3) were designed and qPCR was performed to amplify the genes of interest using the CF96X Real Time System (BioRad, USA). The qPCR data were analyzed using the ΔΔCt method as described previously150 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 ml of supernatant was diluted with 3 ml of distilled H2O and applied to freshly prepared Dowex columns (AG1-X8; Bio-Rad). Columns were washed twice with distilled H2O ...
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bioRxiv - Developmental Biology 2022Quote: ... antibody-bead complexes were washed 3 times with lysis buffer before eluting the samples by boiling for 10 minutes in 4x loading dye (Bio-Rad) containing 10% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... Copy number assay was performed using 3 ng gDNA as a template and commercially available probes for MET (dHSACP2500321, FAM, Bio-Rad) and NSUN3 (dHSACP2506682 ...
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bioRxiv - Pathology 2019Quote: ... and the resulting solutions were applied to a rehydration plate and covered with 17-cm immobilized pH gradient (IPG-immobilized pH gradient) strips for isoelectric focusing (pH 3-10, Bio-Rad). To soak the gel present on the strips with the protein sample ...