Labshake search
Citations for Bio-Rad :
2651 - 2700 of 6899 citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... followed by the iScript cDNA Synthesis Kit (Biorad, #1708891). Quantitative RT-PCR analysis was performed using the SsoAdvanced Universal SYBR Green Supermix kit (Biorad ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were quantitated using a kit from Bio-Rad and loaded onto 10% SDS-PAGE gels and blotted with anti–TGFβ ...
-
bioRxiv - Neuroscience 2023Quote: ... The Clarity Max™ Western ECL Substrate Kit (BioRad) was used and the bands were visualized using the ChemiDoc XRS+ system (BioRad) ...
-
bioRxiv - Immunology 2023Quote: ... The i-Script kit from Bio-Rad (Hercules, CA) was used to generate cDNA ...
-
bioRxiv - Cell Biology 2023Quote: ... Bio-Rad Protein Assay Kit II (Bio-Rad laboratories) was used to measure the acetylated tubulin concentration from the post-nuclear supernatant collected from the lysates ...
-
bioRxiv - Cell Biology 2023Quote: ... iTaq Universal Probes One-Step Kit (Bio-Rad 1725141) master mix was made up according to manufacturers’ instructions ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was prepared with iScript cDNA synthesis kit (Biorad). PCR reactions were run on Quantstudio 5 (Applied Biosystems ...
-
bioRxiv - Neuroscience 2024Quote: ... iScript™ Advanced cDNA Synthesis Kit (BIO-RAD, USA) was used for reverse transcription.
-
bioRxiv - Immunology 2024Quote: ... and the FAM FLICA poly caspase kit (Bio-Rad). Cells were fixed with the Foxp3/Transcription factor staining buffer kit (eBioscience) ...
-
bioRxiv - Cancer Biology 2024Quote: cDNA synthesis was performed with iScript kit (BioRad; #1708891) according to the manufacturer protocol using C1000 Touch Thermal Cycler (BioRad ...
-
bioRxiv - Physiology 2024Quote: ... methods of reverse transcription (iScript cDNA synthesis kit; Biorad), Real Time quantitative PCR (IQ SYBR Green super mix ...
-
bioRxiv - Developmental Biology 2020Quote: ... Upon removal of genomic DNA (TURBO DNA-free™ Kit or RNase-free DNase Set) cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad). PCR reactions were run in triplicate with 6.25-12.5 ng cDNA per reaction using the CFX96 Real-Time PCR Detection System (BioRad) ...
-
bioRxiv - Pathology 2019Quote: ... this amount of protein was broken down via a precipitation and purification kit (ReadyPrep™ 2-D Cleanup Kit, Bio-Rad, Warsaw, Poland). The obtained protein pellets were then dissolved in a rehydration buffer ...
-
bioRxiv - Physiology 2023Quote: ... protein content in the soluble fraction of homogenates was measured using a RC-PC protein assay kit (DC Protein Assay Kit, Bio-Rad, Hercules, CA), and then dissolved in Laemmli buffer.
-
bioRxiv - Neuroscience 2019Quote: ... Reactions were carried out in triplicate on 96-well plates with 10 µL reactions per well using a CFX96 Touch™ Real-Time PCR Detection System from Bio-Rad (Hercules, CA, USA). Cycling conditions for qPCR reactions were as follows ...
-
Gonads or body? Differences in gonadal and somatic photoperiodic growth response in two vole speciesbioRxiv - Neuroscience 2020Quote: ... reactions were carried out in duplo for each sample by using 96-well plates in a Fast Real-Time PCR System (CFX96, Bio-Rad, Hercules, California, United States). Primers for genes of interest were designed using Primer-BLAST (NCBI ...
-
bioRxiv - Immunology 2019Quote: ... 2 µL of the appropriate cDNA template and 18 µL of the reaction mixture were added to the wells of iQ 96-well semi-skirted PCR Plate (Bio-Rad, cat. no. 223-9441). Amplification was achieved using Bio-Rad iQ5 Real-time PCR Detection System ...
-
bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
-
bioRxiv - Immunology 2020Quote: ... Volumes of 2 µL of the appropriate cDNA template and 18 µL of the reaction mixture were added to the wells of iQ 96-well semi-skirted PCR Plate (Bio-Rad. Cat. No. 223-9441). Amplification was achieved using Bio-Rad iQ5 Real-time PCR detection System Relative changes in gene expression was determined using delta/delta cycle threshold (2-ΔΔCT ...
-
bioRxiv - Systems Biology 2022Quote: ... shortly centrifuged (2,000xg, 1 min) and the plate heated at 95°C for 60 min in a thermal cycler (Biorad’s S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
-
bioRxiv - Immunology 2023Quote: ... Plasma was thawed on ice and manufacturers instructions were followed to prepare a 96-well plate with samples performed in duplicates and read on a Bio-Plex® 200 System (Bio-Rad Laboratories, Hercules, CA). Results were calculated using Bio-Plex Manager™ Software v6.2 (Bio-Rad ...
-
bioRxiv - Plant Biology 2024Quote: ... Cycling conditions were optimized by performing 10 µl reactions containing 5.0 µl assay mastermix and 5.0 µl of parental DNA and two non-template water controls (NTCs) in 384-well plate format on a CFX384 qPCR thermocycler (Bio-Rad Laboratories, Hercules, CA, USA). Cycle number was optimized by assessing every two cycles between 26-36x cycles post touchdown of 10 cycles (61–55°C ...
-
bioRxiv - Genetics 2024Quote: Quantitative PCR (qPCR) was performed in a 96-well plate using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, US) with Bio-Rad EvaGreen qPCR Supermix assay ...
-
bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Real time quantitative PCR (qRT-PCR) was performed using an iQ™5 Multicolor Real-Time PCR Detection System (BIO-RAD) with qPCRBIO SyGreen Mix fluorescein (PCR BIOSYSTEMS) ...
-
bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
-
bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
-
bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
-
bioRxiv - Genetics 2019Quote: ... and heated at 90°C for 5 min before being run on a stain free gel and imaged by GelDoc imager (BIO-RAD).
-
bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semi-dry transfer (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
-
bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
-
bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
-
bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
-
bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
-
bioRxiv - Physiology 2019Quote: For serum fetuin-A analysis murine serum was separated by sodium dodecyl sulphate polyacrylamide gel elecrophoresis using mini gels (10% acrylamide, 5 × 8 × 0.1 cm3, BioRad, Hercules, USA). Protein transfer onto nitrocellulose membrane was by semi-dry electroblotting (Owl HEP-1 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
-
bioRxiv - Neuroscience 2019Quote: ... Equal amounts of protein per sample (5 or 10ug) was loaded into a 4-20% gradient precast mini-PROTEAN TGX gel (Bio-Rad) and proteins were separated with 200V in 1x running buffer (24.76 mM Tris base ...
-
bioRxiv - Developmental Biology 2020Quote: ... excess antibody subsequently removed by a further 4 washes in TBSTw for 5 minutes before detection with ECL Western blotting reagent (Bio-Rad) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: T1L virions (2×1012 particles/ml) were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of the reaction was 30 μl in virus storage buffer (10 mM Tris ...
-
bioRxiv - Cancer Biology 2019Quote: ... transferred onto PVDF membrane and probed with primary antibody (Table 1) at a dilution of 1:1000 prepared in 5% blotting grade blocker (Bio-Rad) for 12-16 h at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
-
bioRxiv - Bioengineering 2021Quote: ... This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad) for 1 h with rocking ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
-
bioRxiv - Neuroscience 2021Quote: ... 50 μg of neuronal lysate and corresponding mHTTex1 seed were boiled for 5 min with sample loading buffer and loaded into a 4-20 % polyacrylamide gel (SDS-PAGE, Criterion Bio-Rad) to detect monomeric endogenous HTT or by semi-denaturing detergent agarose (1.5% ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...