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Citations for Bio-Rad :
2651 - 2700 of 9612 citations for 7 7a Dihydro 2 2 4 6 6 pentamethyl 1 3 benzodioxol 5 6H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Viral genomes were quantified by Taqman one-step qRT-PCR on a CFX96 Touch real-time PCR detection system (BioRad) and were reported on a log10 scale measured against standard curves from either a ZIKV A-plasmid as previously described (69) ...
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bioRxiv - Microbiology 2022Quote: cDNA was prepared for RT-qPCR by reverse transcribing one microgram of the extracted RNA using iScript Reverse Transcription Supermix (BioRad). qPCR was performed using iTaq-Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Microbiology 2022Quote: HCV RNA copies were determined by Taqman RT-qPCR using a Quanta BioSciences qScript XLT One-Step RT-qPCR kit according to the manufacture’s instruction (Bio-Rad). GLT1_probe was used to detect for GLT1 RNA ...
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bioRxiv - Immunology 2021Quote: ... The gel image was acquired in the Gel Doc 1000 system and analyzed using the Quantity One software (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
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bioRxiv - Evolutionary Biology 2020Quote: ... or ∼50 ng (8-14 months post-hatch) of total RNA as template in one-step reverse transcription droplet digital PCR (RT-ddPCR) [66] (BioRad) with TaqMan probes specific to vgll3 alleles to quantify vgll3 absolute expression (Supplementary table 1) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad) system ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein band of interest was subsequently quantitated (normalized to the reference protein band) using quantity one software (Bio-rad). Antibodies used for western blot analysis are listed in Suppl ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (12.5 ng) was reverse transcribed and amplified with the iTaq Universal SYBR Green One-Step Kit (Bio-Rad). RUNX1 and ACTB were amplified in parallel in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... 12.5-50 μg of protein was mixed with one third volume of 4X Laemelli Sample Buffer (Bio-Rad Laboratories 1610747) and boiled at 100 °C before loading into a polyacrylamide gel alongside either the PageRuler™ or PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Cell Biology 2021Quote: ... Gene expression analysis was conducted with 50 ng RNA using iTaq Universal One-Step RT-qPCR Kit (Bio-rad, 1725140). The reaction was carried out according to the manufacturer’s instructions using CFX Connect Real-Time PCR Detection System (Bio-rad ...
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bioRxiv - Microbiology 2019Quote: ... ZIKV RNA levels were determined by TaqMan one-step quantitative reverse transcription PCR (qRT-PCR) on a CFX96 Touch Real-Time PCR Detection System (BioRad) using standard cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The immunodetected protein bands were then analyzed using ChemiDoc XRS system with Quantity One software (Bio-Rad, Richmond, CA, USA).
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bioRxiv - Genomics 2021Quote: ... 20 μl ddPCR mix consisted of 5μl One-Step RT-ddPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 μl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and cDNA amplification were done using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA) and a CFX96 real-time system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... One half microgram of total RNA was reverse transcribed into cDNA using an iScript cDNA Synthesis kit (Bio-Rad, 1708891). All TaqMan primers and probes were obtained from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... One µg of total RNA was used for first-strand cDNA synthesis with the iScript cDNA Synthesis Kit (Bio-Rad) and diluted 8 times afterwards ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA levels were determined by TaqMan one-step qRT-PCR on a CFX96 Touch Real-Time PCR Detection System (BioRad) using standard cycling conditions ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 6-week-old rosette leaves and bolting flower buds of Col-0 and one of the RPF2-atp1 (RPF2-atp1-9) transformed lines (T3) with PureZol reagent (BioRad). Three independent libraries for each genotype were made from total RNA treated with 250 ng of Turbo DNase (Ambion ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100ng of RNA was used with iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad, CA, USA) to perform a one-step RT-qPCR in a volume of 20 μl using a QuantStudio3 real-time PCR instrument from ThermoFisher (Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
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bioRxiv - Cancer Biology 2024Quote: ... Conversion of one µg of RNA into cDNA was performed using the iScript cDNA Synthesis Kit (1708890; Bio-Rad Laboratories) and Master Cycler Pro Device (EPPE6324000.516 ...
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bioRxiv - Molecular Biology 2023Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaqTM universal SYBR® Green One-Step kit (Bio-Rad): 95 °C for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of one μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA was used to perform the reverse transcriptase reaction with iScript TM cDNA Synthesis Kit (Bio-Rad). Changes in mRNA levels of the target genes were determined by relative RT-qPCR with a CFX96TM Real‒Time PCR Detection System (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng of purified RNA was immediately reverse transcribed at 37 °C for one hour in an S1000 Thermal Cycler (BioRad) via the OmniScript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Transcripts were measured by TaqMan one-step qRT-PCR on a CFX96 Touch real-time PCR detection system (Bio-Rad) and were reported as relative differences in threshold cycle (Ct ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction mixture was separated on SDS-PAGE and the phosphorylated receptor was autoradiographed for image analysis with the Quantity One program (BioRad) on phosphor screens.
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... pH 9.5). Quantification of Western blot images (SFig. 1-4) was carried out using ChemiDoc™ XRS+ Image Lab™ Software (Bio-Rad Laboratories AB, Sweden); statistical analysis was performed with GraphPad Prism software (version 5.0 ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated overnight at 4°C with antibody cocktail consisting of rat anti-F4/80 (1:100) (catalog #: MCA497; Bio-Rad Laboratories; Hercules, CA, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Plant Biology 2019Quote: 5 % Mini-PROTEAN TBE precast gels (Bio-Rad) have been pre-electrophoresed in 0.5x TBE buffer for 60 minutes at 70 V ...
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bioRxiv - Cancer Biology 2022Quote: ... PVDF membranes were blocked with 5% milk (BioRad) in TBST and then probed with listed antibodies diluted in either 1% BSA (anti-phospho-specific antibodies ...
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bioRxiv - Microbiology 2019Quote: ... loaded onto a 5% polyacrylamideTBE gel (Bio-Rad) that had been pre-run for 15 minutes in TB Buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... in 5% powdered milk (Bio-Rad 170-6404) resuspended in 1x TBST ...
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bioRxiv - Genomics 2019Quote: ... Blocked membrane in 5% Blotting-Grade Blocker (BioRad) in TBS-T (50 mM Tris pH 7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5%-milk (Biorad 1706404) for 30 minutes and washed in PBS/Tween20-0.05% ...
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bioRxiv - Neuroscience 2022Quote: ... After being blocked in 5% milk (Bio-Rad), the membrane was incubated with primary anti-tau polyclonal antibody (Dako ...
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bioRxiv - Genomics 2022Quote: ... and 5 kbp ladder (BioRad,Cat #170–3624) were used as standards ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-tetramethylbenzidine (TMB Peroxidase EIA Substrate Kit, Biorad), a stop solution (H2SO4 2M ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA extractions using a 5% Chelex (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... and 5% beta-mercaptoethanol (Bio-Rad, Hercules, CA) and separated by SDS-PAGE with NuPAGE 4-12% Bis-Tris 1.5 mm 15-well gels (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 μl of 4X Laemmli loading dye (BioRad) was added to 15 μl of either total lysate ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of 4X loading buffer (Bio-Rad) was added ...
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bioRxiv - Biochemistry 2022Quote: ... with ImageLab Version 5 (Bio-Rad, Hercules, CA). Each experiment has three independent repeats.
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Sosofast Eva Green (Bio-Rad) 2X master mix ...
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bioRxiv - Developmental Biology 2023Quote: ... Non-denaturing 5% polyacrylamide 1xTBE Criterion gels (BioRad) were pre-run 30 minutes before loading the reactions.
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bioRxiv - Immunology 2024Quote: ... 72°C for 5 minutes (BioRad, Hercules, CA). Genomic PCR was performed using the following primers ...