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Citations for Bio-Rad :
2651 - 2700 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Equal numbers of cells were loaded onto the gel in 2× Laemmli Buffer (BioRad, #1610737), and proteins were loaded onto 18-well BioRad anyKD Criterion TGX gels (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The TaqMan PCR reaction mixture was assembled from a 2× ddPCR master mix (Bio-Rad), 20× primers and probes (final concentrations of 900 and 250 nM ...
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bioRxiv - Bioengineering 2023Quote: ... 35 µL N,N-methylenebisacrylamide crosslinker (2% w/v bis-acrylamide Solution, 1610142, Bio-Rad), 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm ...
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bioRxiv - Neuroscience 2023Quote: ... anti-CD8 (2 µg/rat dissolved in saline solution, clone OX-8, Bio-Rad #MCA48G) or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution ...
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bioRxiv - Biochemistry 2023Quote: ... To a solution of Affi-Gel 10 (Bio-Rad, 2 ml, 0.03 mmol, 1.0 eq) in a solid phase synthesis tube with frit was added a solution of compound 11 (27.7 mg ...
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bioRxiv - Developmental Biology 2024Quote: ... and the supernatant was mixed with equal volume 2 x Laemlli Buffer (Bio-Rad, 1610737) and heated to 95 °C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell suspension was mixed with 50 µL molten 2% CleanCut agarose (Bio-Rad) after which the mixture was pipetted into a plug mold (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μg of total RNA was used to make cDNA using iScript reverse transcriptase (BioRad) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1hr on ice with varying concentrations of fluorescently labeled hybridoma-derived or recombinant mAbs and then washed x3 in flow buffer (PBS + 3% fetal calf serum) prior to analysis on a ZE5 flow cytometer (BioRad, Hercules, CA). Equilibrium binding affinities were calculated via nonlinear regression (One site – total and non-specific binding ...
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bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml Tween20 add to 1000 ml with ddH2O) 3 times for 20 min and blocked with 10% dried milk-TBST solution or BlockAce-TBST solution (BlockAce, Bio-Rad, #170223) for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
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bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... (see Supplementary Table 3 for a full list of oligos used in the present study) into 384-well hard-shell PCR plates (Bio-Rad HSP3901) using Mantis liquid handler (Formulatrix) ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
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bioRxiv - Pathology 2019Quote: ... F4/80 (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100), CD3 (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
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bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
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bioRxiv - Cell Biology 2020Quote: ... destained for 2 h at room temp (Coomassie Brilliant Blue R-250 staining kit, BIO-RAD), and imaged on a ChemiDoc MP (BIO-RAD).
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were then transferred (200 mA for 2 hours) to polyvinylidene difluoride membranes (Bio-Rad Laboratories), Ponceau stained ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated small RNAs were separated from HPDP-biotin and pyridine-2-thione using spin columns (BioRad) in ultra-pure water.
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bioRxiv - Bioengineering 2021Quote: ... and the selected primers (Table 2) using a CFX Connect™ Real-Time System (Bio-Rad) under the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: anti–α-tubulin (MCA77G YL1/2, rat monoclonal; Bio-Rad), anti-GAPDH (mouse ...
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bioRxiv - Cell Biology 2021Quote: Samples for electrophoresis were prepared by mixing with 2× Laemmli sample buffer (Bio-rad, Hercules, California) and denatured at 55°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad). The radiolabeled probe was heated to 100°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesised from 2 µg purified RNA using iScript™ cDNA Synthesis Kit (Bio-Rad) and quantified by qRT-PCR using iTaq™ Universal SYBR® Green Supermix ...
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bioRxiv - Cancer Biology 2020Quote: ... Two percent agarose gel was prepared by dissolving 2 g Agarose (Bio-rad, Cat No. 1613102) and 2 µL SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad, 1610737) at 95 °C for 8-10 min ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates were diluted to 2 µg/µl in Laemmli buffer (Bio-Rad, Hercules CA, 1610747) and heated at 95ºC for 5 minutes and resolved using a 4-20% tris-glycine gel (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were mounted on slides in reagent 2 in frame-seal incubation chambers (Bio-Rad SLF0601) and coverslipped ...
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bioRxiv - Biophysics 2020Quote: ... Detergent was slowly removed by the addition of SM-2 Bio-Beads (Bio-Rad, Hercules, CA) over a 4-hour time course (final weight ratio of 25 Bio-Beads to 1 detergent) ...
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bioRxiv - Microbiology 2021Quote: ... each cDNA was combined with segment-specific primers (Table 2) and Precision Melt Supermix (Bio-Rad) and analyzed by qPCR using a CFX384 Touch real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... mixed thoroughly and the mixture transferred to an electroporation 2 mm-gap width cuvette (Bio-Rad). Electrotransformation was performed in a Micropulser™ device (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... and probed with each primary antibody diluted in 2% blotting-grade non-fat dry milk (BioRad). Next ...