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Citations for Bio-Rad :
2651 - 2700 of 5172 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... rat anti-Cd11b (Biorad #MCA74G, 1:100), CD68 (Biolegend #137020 ...
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bioRxiv - Bioengineering 2024Quote: ... 29:1 was from Bio-Rad (USA). DFHBI-1T was purchased from Tocris Bioscience (UK) ...
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bioRxiv - Bioengineering 2024Quote: ... anti-Rat Ly6b2 (BioRad, MCA771G, 1:200); anti-Goat ChAT (MilliporeSigma ...
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bioRxiv - Neuroscience 2024Quote: ... anti-mouse Cd68 (1:200, BioRad, #MCA1957), anti-human CD68 (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Alix (Biorad MCA2493, 1:1000), rabbit anti-Calnexin (Enzo SPA-860 ...
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bioRxiv - Neuroscience 2024Quote: ... CD68 (1:300, BIO-RAD, Cat# MCA1957T), CD45-APC (1:200 ...
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bioRxiv - Plant Biology 2024Quote: ... 37.5:1 (Bio-Rad, catalog no. 1610148). RNA samples were mixed (1:1 ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-MBP (Biorad #MCA409S, 1:500), mouse anti-Nkx2.2 (DSHB #74.5A5-c ...
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bioRxiv - Cell Biology 2024Quote: ... anti-TGN46 (1:500 IF, Biorad; AHP500), anti-VPS26A IF ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-rat Ly6B2 (1:500, Biorad), were incubated at 4 °C overnight ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 ng of cDNA were used in each qPCR reaction on a CFX Connect Real-time PCR detection system (Bio-Rad, Hercules, CA) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8 μm cryostat sections of C51 and F9 tumors stained with IgG (L19)-FITC (final concentration 2 μg/mL) and detected with a Rabbit anti-FITC (Bio-Rad; 4510-7804) and Donkey anti-Rabbit AlexaFluor 488 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blood was first separated from the ethanol through gentle centrifugation and embedded in molten 2% agarose plugs (Bio-Rad DNA plug kit). The solidified plugs were submerged in Lysis Buffer solution (Bionano ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Cell Biology 2021Quote: ... were amplified using Phusion High-Fidelity PCR MasterMix (primer sequences are listed in Additional File 2) and a C1000 thermocycler (Bio-Rad Life Science). The peak sequences were amplified for 98 °C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... and Reverse Transcription (RT) was performed with 2 µg RNA per 40 µl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Biophysics 2022Quote: ... the samples were cooled on ice for 10 min followed by the addition of 80 mg Bio-beads SM-2 Resin (Bio-Rad, Hercules, CA), then rotated at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... ddPCR was used to detect DNA of SP by using a QX100 Droplet Digital PCR System and 2× ddPCR Supermix (Bio-Rad, California, CA) with protocols developed in our previous studies 48-57 ...
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bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate/bead mixtures were incubated at 4°C on a rotating platform for 2 hrs and subsequently poured into Poly-Prep® Chromatography Columns (Bio-Rad #7311550). Unbound fractions were collected through the columns and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... and Reverse Transcription (RT) was performed with 2 μg RNA per 40 μl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Genetics 2022Quote: ... a small implant of the thallus of interest (0.5 cm2) was set in 2 mL tube containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Pathology 2019Quote: ... this amount of protein was broken down via a precipitation and purification kit (ReadyPrep™ 2-D Cleanup Kit, Bio-Rad, Warsaw, Poland). The obtained protein pellets were then dissolved in a rehydration buffer ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Immunology 2020Quote: ... The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories, USA) with 2 mM ATP (MP Biomedicals ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
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bioRxiv - Molecular Biology 2021Quote: ... and then liposomes were formed by the step-wise removal of pentaethylene glycol monodecyl ether by five additions at 20 min intervals of 60 mg Bio-Beads SM-2 (Bio-Rad, Watford, UK) with gentle mixing at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Round spermatids were sorted based on 1C DNA content and size using a 2-laser sorter (BioRad S3e with 488nm and 561nm lasers) gated on single cells as previously described (Lesch et al. ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were resolved by SDS-PAGE and transferred to a PVDF membrane by tank blotting at 150 mA for 2 h using the Miniprotean III System from Bio-Rad (Hercules, USA). The membrane was blocked for 1 h with 5% skim milk in PBS containing 0.05% Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38) before running on a CFX96TM real-time PCR cycler (BioRad, Hercules, CA, USA) under cycle conditions of 42°C for 5 min ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Plant Biology 2022Quote: ... 50 mg of powder was transferred into a 1.5 mL microcentrifuge tube with 150 ul of 2 x Laemmli buffer (Bio-Rad, Hercules, CA, USA). The sample was immediately vortexed for 1 min followed by incubation at 70°C for 10 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The assembled products were mixed with electrocompetent cells and transformed into electrocompetent cells in a 2-mm Gene Pulser cuvette (Bio-Rad, 165-2086) using an electroporation instrument (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg (islet sample) or 2 µg (mouse liver sample: positive control) was mixed with 10uL of Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and denatured at 95°C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: RT-PCR was performed after generating first-strand cDNAs from 2 µg of total RNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA), as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: mMEC and hMEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Immunology 2023Quote: ... The amplified products were analyzed on a 2% agarose gel stained with SYBR safe and imaged using the Gel-documentation system (Bio-Rad, Hercules, CA).
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...