Labshake search
Citations for Bio-Rad :
2551 - 2600 of 7747 citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... About 80 µg of denatured proteins were loaded for each condition and separated on gradient 4–12% polyacrylamide classic or TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
-
bioRxiv - Molecular Biology 2020Quote: Whole flies or human primary cell pellet was homogenised in 2x Laemmli loading sample buffer (100 mM Tris pH 6.8, 20% glycerol, 4% SDS; Bio-Rad) containing 50 mM DTT ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 14,000×g for 30 min at 4°C and equalized using quick start Bradford 1x dye reagent (BioRad, 5000205). Ni-NTA resin was washed three times with lysis buffer containing 20mM imidazole pH 8.0 and 5mM β-mercaptoethanol while samples were prepared ...
-
bioRxiv - Cell Biology 2022Quote: ... using the BioRad transfer system at 100 V for 60 min at 4°C or with Trans-Blot Turbo Mini PVDF Transfer Packs using Trans-Blot Turbo Transfer System (Biorad). The membrane was blocked for 30 min with the Odyssey Blocking Buffer (TBS ...
-
bioRxiv - Cell Biology 2022Quote: ... supernatants aspirated and loaded on to 4-12% NuPage Bis/Tris gels for separation before transfer on to a Nitrocellulose membrane (Biorad) at 4°C at a constant current of 350mA in transfer buffer (25 mM Tris ...
-
bioRxiv - Immunology 2021Quote: ... were separated on a 4-20% SDS-PAGE gel and blotted onto a PVDF membrane using a Trans-Blot Turbo transfer system (BioRad). Membranes were blocked for 1h in 5% skim milk in TBST and then probed with an anti-S2 mouse monoclonal antibody (GeneTex ...
-
bioRxiv - Cell Biology 2019Quote: ... Polyacrylamide gels used in this study were either prepared using 10% EZ-Run protein gel solution (Fisher) or precast gradient gels (4-20%, from Biorad). Any quantification performed on western blots was done using ImageJ software ...
-
bioRxiv - Molecular Biology 2019Quote: ... Samples (15 μL) were loaded onto pre-casted gradient (4-15%) SDS-polyacrylamide gels (Bio-Rad Laboratories; Hercules, CA, USA) and subjected to electrophoresis at 180 V for 60 minutes using pre-made 1x SDS-PAGE running buffer (Ameresco ...
-
bioRxiv - Plant Biology 2019Quote: Proteins were separated on denaturing SDS-PAGE followed by an electrotransfer at 4 °C onto a PVDF membrane (0.2 µM, Bio-Rad). NRT2.1 was detected using three different anti-NRT2.1 antisera produced by Eurogentec against either the synthetic peptide TLEKAGEVAKDKFGK (anti-NRT2.1 19) ...
-
bioRxiv - Genetics 2020Quote: ... Samples were centrifuged at 17,000 × g for 30 min at 4 ºC and the supernatant was run on a 10% Mini-PROTEAN TGX precast polyacrylamide gel (BioRad). Protein was transferred to a PVDF membrane using the iBlot 2 (Life Technologies) ...
-
Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... Immunoblotting was performed with 4-15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and nitrocellulose membranes (Bio-Rad, Hercules, CA). Membranes were blocked with 5% milk in TBS with 0.1% Tween 20 (TBST ...
-
Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4– 15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h ...
-
bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h and then incubated with primary overnight and secondary antibodies for 2 h ...
-
bioRxiv - Molecular Biology 2019Quote: ... Samples were boiled at 95 °C for 10 mins with 6×SDS sample buffer before loading onto 4–20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
-
bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h in nonfat-milk ...
-
bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged at 30,000 x g for 30 min at 4°C before being loaded into a gravity flow column (Bio-Rad) and purified by Ni-NTA resin (UBP Bio ...
-
bioRxiv - Genetics 2021Quote: ... rotated at 4°C for 2h and then poured into a Econo-Column® Chromatography column (Bio-Rad, Hercules, CA). After extensive washing first with 80 mL of 20 mM Tris HCl [pH8@4°C] ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Samples were run on a 4–20% SDS-PAGE and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories) using a semidry blotting apparatus (Bio-Rad Laboratories) ...
-
bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2020Quote: ... The testicular extract was run on a SDS-PAGE 4-15% mini-protean TGX precast gel (Bio-Rad, 456-1084) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Immunology 2021Quote: ... The material bound on the beads was eluted in Laemmli buffer and the proteins were separated by SDS-PAGE using Criterion 4-15% acrylamide gels (BioRad) in Tris-Glycine-SDS buffer ...
-
bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysate was centrifuged at 30,000 x g for 30 min at 4 °C before being loaded into a gravity flow column (Bio-Rad) and purified by Ni-NTA resin (UBP Bio ...
-
bioRxiv - Bioengineering 2020Quote: ... NJ) overnight at 4°C and visualized using the Bio-Rad ChemiDoc MP Imaging System (Bio-Rad Laboratories, Hercules, CA).
-
bioRxiv - Biochemistry 2021Quote: ... respectively 45 μL and 10 μL of proteins were loaded for each condition and separated on gradient 4-12% polyacrylamide TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
-
bioRxiv - Biochemistry 2021Quote: ... and electroporated (2500 V, 200 Ω, 25 μF, exponential decay, time constant < 4 ms) using Gene Pulser Xcell (Bio-Rad). 1 mL BHI medium + 0.5 mM saccharose was added and the cells were incubated at 30 °C for 4 h and plated on BHI agar plates containing 10 μg/mL erythromycin ...
-
bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
-
bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Biochemistry 2020Quote: Cross-linked samples of purified ROS-GC1 fragment (aa 814-1110) were analyzed by SDS-PAGE using gradient gels (4-20%, Mini-PROTEAN TGX gels, Biorad). For in-gel digestion ...
-
bioRxiv - Cancer Biology 2020Quote: Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...
-
bioRxiv - Neuroscience 2020Quote: ... Approximately 12 μg of total protein were loaded onto a 4-20% mini Protean TGX gel (Bio-Rad, Hercules, CA) and blots were evaluated as described previously
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded in a 10-well 4-20% Mini-PROTEAN TGX Precast protein gel (BIO-RAD, Catalog #4561094). The gel was run at 150V for 5 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... and then resuspended in 60 μl lysis buffer and 20 μl 4 x Laemmli Sample Buffer (Bio-RAD, 161-0747), heat shocked in a thermomixer (Eppendorf ...
-
bioRxiv - Immunology 2021Quote: ... Cell lysates were separated by protein electrophoresis at 150 V for 1h using 4-20% Mini-Protean TGX pre-cast gels (BioRad) and transferred by semi-dry technic onto Amersham Hybond-P PVDF membranes (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were spun down and loaded onto a 4-20% (w/v) polyacrylamide precast mini-PROTEAN TGX gel (Bio-Rad) for subsequent gel-electrophoresis ...
-
bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted in SDS page loading buffer and separated on 4-15% Mini-PROTEAN TGXTM Precast Protein Gels (BioRad). Samples were processed for immunoblotting or fixed and stained for mass spectrometry using Imperial protein stain (Pierce ...
-
bioRxiv - Physiology 2021Quote: ... or chylomicron fractions were mixed with 4x laemmli buffer and loaded onto 4%–15% TGX Gels (Bio-Rad, Hercules, CA), transferred to hybond PVDF membrane (GE Healthcare ...
-
bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay ...
-
bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified and the same amount of protein was loaded to the wells in a 4-15% SDS-PAGE gel (BioRad). Protein was transferred to a PVDF membrane (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: SDS-PAGE and western blotting were performed using 4-20 % Mini-PROTEAN TGX Stain-free Precast Gels (Bio-Rad, 4568094) and Trans-Blot Turbo 0.2 μm nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
-
bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein sample was incubated at 98°C for 5 min and ran on Bio-Rad TGX Stain-Free Precast Gel (4-15%) in Tris/Glycine/SDS running buffer (BioRad). Then the gel was transferred onto a 0.45 μm PVDF membrane (Thermofisher) ...
-
bioRxiv - Microbiology 2022Quote: ... Ten micrograms of protein was run per sample on 4–15% Mini-Protean TGX stain-free precast gels (Bio-Rad) at 150 V ...
-
bioRxiv - Microbiology 2022Quote: ... total protein lysates of T.brucei BSF were separated by SDS-PAGE (4-20% Mini PROTEAN TGX stain-free precast gradient gels, Bio-Rad) and blotted on PVDF filters (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2022Quote: ... P14 and P18 retinas were measured by fluorescent Western blot analysis [4-15% or 7.5% Mini-PROTEAN TGX gels (Bio-Rad)] and quantitation was performed using the LICOR (LICOR ...
-
bioRxiv - Biochemistry 2022Quote: ... protein samples were separated by precast 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad Laboratories 5678085), transferred to a nitrocellulose membrane using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad Laboratories 1704159EDU ...
-
bioRxiv - Cell Biology 2022Quote: ... The whole cell lysate and crude cytoskeletons were resolved on 4-20% Mini-PROTEAN tris-glycine polyacrylamide gels (Bio-Rad), and Western blots were stained with 1:5000 diluted anti-beta-actin mouse monoclonal antibody (Cell Signaling Technology ...