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Citations for Bio-Rad :
2501 - 2550 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Cell lysates were prepared at the indicated time points post-infection in Laemmli sample buffer (BioRad). The samples were boiled for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... at 100 V for 1 h at room temperature using Mini Trans-Blot Cell system (Biorad). Fast Green staining was used to detect the whole protein content on the membrane ...
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...
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bioRxiv - Cell Biology 2022Quote: BN-PAGE gels were transferred to PVDF membranes using a Mini Trans-Blot Cell (Bio-Rad). After wet-transfer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice with cold wash buffer and analyzed using a ZE5 cytometer (Biorad) with acquisition chamber T= 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell extracts were sonicated 10 s and protein concentration determined using DCTM Protein Assay (Bio-Rad). Ten micrograms of proteins were loaded and run on NuPAGE 3-8% Tris-Acetate (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in monomer solution (1 x PBS, 2 M NaCl, 2.5% acrylamide (Bio-Rad), 0.15% Methylenbisacrylamide (Santa Cruz) ...
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bioRxiv - Physiology 2020Quote: ... conditioned media from cells was passed through columns containing AG1-X8 Anion Exchange Resin (Bio-Rad) and collected in scintillation vials ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cell lysate protein was quantified with a modified BCA protein assay using manufacturers protocol (Bio-Rad). Samples (20-30 μg ...
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bioRxiv - Microbiology 2019Quote: ... Electroblotting was carried out using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) for 2 h at a constant current of 200 mA ...
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bioRxiv - Microbiology 2020Quote: ... Gels were run at 4°C in a Protean II xi Cell electrophoresis system (Bio-rad) at 40 mA/gel with a running buffer consisting of 100 mM Tris-tricine pH 8.2 until the bromophenol blue loading dye was near the bottom of the gel ...
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bioRxiv - Immunology 2020Quote: cDNA was generated from sorted B cells by adding 4µl of iScript (Bio-Rad Cat# 1708891) and cycling according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the plate was placed on ice and the cells were lysed in 1X Laemmli buffer (Biorad) containing 5% β-mercaptoethanol (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to PVDF (GE-Healthcare) membranes by Trans-Blot Semi-Dry Transfer Cell (Bio-Rad). Membranes were blocked with 5% BSA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Whole cell lysates were collected in standard RIPA buffer and quantified using a Bradford assay (BioRad). Protein lysates (50µg ...
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bioRxiv - Genomics 2019Quote: ... Cell lysates (20 μg) were separated on 4–20% precast polyacrylamide gels (Bio-Rad, Hercules, CA) and were transferred to nitrocellulose membranes ...
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bioRxiv - Immunology 2020Quote: ... and single-cell sorted into 96-well PCR plates (free of DNase and RNase, Bio-Rad). The Plates were stored at −80 °C until BCR Cloning ...
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bioRxiv - Physiology 2021Quote: ... into the lanes of a 5% stacking-and 10% separating polyacrylamide gel (Biorad, MiniProtean Tetra cell). The proteins were separated at 60 V for 30 min and 150 V until the Hb fraction (∼16 kDa ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were transferred into ice-cold 2 mm electroporation cuvettes (Bio-Rad, Hercules, United States) and electroporated (2500 V ...
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bioRxiv - Developmental Biology 2021Quote: ... cultures were dissociated with Accutase and analyzed by flow cytometry using an S3 cell sorter (Biorad). Undifferentiated ESCs or iPS cells ...
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bioRxiv - Cell Biology 2021Quote: Measurements of cell density and viability were performed using trypan blue (Bio-Rad, Hercules, CA, USA) and an automatic cell counter (model TC20 ...
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bioRxiv - Microbiology 2020Quote: ... The cell suspension containing plasmid DNA was electroporated using a Gene Pulser electroporator (Bio-Rad, USA) with the following parameters ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were index sorted using a BD FACS Influx into 384-well hardshell plates (BioRad) that were pre-filled with 5 µL of light mineral oil (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with cold wash buffer and analyzed using a ZE5 cytometer (Biorad) with acquisition chamber T= 4°C ...
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bioRxiv - Genomics 2022Quote: ... Gels were ran at 100-200V in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Biorad 1658004). Proteins were transferred to Immobilon-FL PVDF (Millipore IPFL00010 ...
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bioRxiv - Cell Biology 2022Quote: ... Bands were visualized by chemiluminescence using enhanced chemiluminescent (ECL) substrate (BioRad, #170-5061/Cell Signaling, #12757S).
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bioRxiv - Microbiology 2022Quote: ... stained with Trypan blue using standard procedures and analyzed with a TC20TM Automated Cell Counter (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 0.3% SDS and 20% methanol) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) set at 100 mA constant power with a volt limit of 25 V for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... the samples (cells with plasmid) were added to a 2mm-gap electroporation cuvette (Bio-Rad, USA). The sample was pulsed at 140V and 1,000 μF with an exponential decay on a Bio-Rad GenePulserXcell module ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were stained with the fluorescent inhibitor probe FAM-YVAD-FMK (FAM 660, BioRad, Germany) for 30 minutes at 37°C according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and cell lysates were resolved on Mini-PROTEAN TGX Stain-Free™ precast gels (Bio-Rad). For immunoblotting ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated hBMSCs-derived cells were transfected using the Gene Pulser-II Electroporation System (Bio-Rad Laboratories). Electroporation was performed in a sterile cuvette with a 0.4-cm electrode gap (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2023Quote: ... 30μg whole-cell extracts were separated by SDS–PAGE and transferred onto Nitrocellulose membrane (Bio-Rad). After blocking in 5% milk in TBS-T ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stained with trypan blue and counted using Automated Cell Counter (BioRad, Hercules, California, USA). PBMCs were then cryopreserved in a cryovial in cell recovery freezing medium containing 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2022Quote: ... We dissociated the cells and extracted RNA using the Aurum Total RNA mini kit (Biorad, 7326820). We measured RNA concentration using a spectrophotometer and only included RNA that had an A260/280 >1.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Identical number of cells from each sample was collected and electrophoresed on polyacrylamide gels (Bio-Rad). Antibodies used were as follows ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the cell extracts was determined using the BCA assay kit (Bio-Rad). Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... with BD FACS Melody instrument for the first round library and S3e Cell Sorter (Bio-Rad) for the second round library ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysates were then collected and protein concentration was determined using the Bradford method (Bio-Rad). For total protein analysis ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from cells and tissue using Aurum™ Total RNA Mini kit (Bio-Rad) and reverse transcribed using iScript™ cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Viability of cryo-conserved cells was evaluated using trypan blue (BIO-RAD, Marnes-La-Coquette, France) using the automated cell counter TC20 (BIO-RAD) ...
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bioRxiv - Genomics 2024Quote: 20µg protein was resolved by SDS-PAGE in a Mini-PROTEAN vertical electrophoresis cell (Bio-Rad) alongside PageRuler Prestained Protein Ladder (Thermo Scientific #26619) ...
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bioRxiv - Immunology 2024Quote: ... An equal number of cells were collected and lysed in Laemmli Sample Buffer (#1610737, Bio-Rad) containing Pierce Protease and Phosphatase Inhibitor (#A32959 ...
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... cells were allowed to settle on laminin coated coverslips and line scan confocal microscopy mode (Biorad) used to monitor calcium transients with a 488 nm excitation laser ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein concentration of the cell lysate was estimated by Bradford assay (Bio-Rad, Hercules, CA). For Western blotting equal amounts (up to 80 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected by electroporation in OPTI-MEM I using Gene-Pulser II (Bio-Rad) with 960 μF und 0.18 kV with 10 μg pcDNA3-FLAG-JUNB ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were transformed into onion (Allium cepa) epidermal cells using a particle delivery system (BIO-RAD) as previously described (Yang et al. ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates were loaded onto Any kD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad) and separated by electrophoresis ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: 25 μg of EVs or cell lysates was mixed with Laemmli SDS sample buffer (Bio-Rad), incubated 10 min at 95°C ...