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Citations for Bio-Rad :
2451 - 2500 of 4802 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resuspended in a small amount of buffer (6 M urea, 50 mM Tris–Cl pH 7.5) and quantified by the BCA quantification method (Bio-Rad, Hercules, CA). Urea concentration was reduced to 4 M and the pH was adjusted to 8.8 with 25 mM Tris–HCl ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane homogenates (50 μg) were separated on 6% SDS-PAGE gels and transferred onto presoaked PVDF membranes (Bio-Rad, Pleasanton, CA, USA). Blots were then blocked with 10% non-fat milk in PBST (100 nM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories, Hercules, CA, USA). To avoid aggregation ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Molecular Biology 2022Quote: ... The bombardment was performed (a distance of 6 cm using 1350 psi rupture disks) with a PDS-1000/He Biolistic Particle Delivery system (Bio-Rad, USA). The antibiotic concentration was determined after a sensitivity test ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
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bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE and size exclusion chromatography (SEC) using a Superose® 6 Increase 10/300 GL column (Cytiva) and an NGC Quest 10 Plus System (Bio-Rad) showed that both wild-type and variant proteins were pure and monodisperse (Figure 2-figure supplement 1).
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bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272, BioRad, Switzerland) under the following conditions ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by heat-inactivation at 75°C for 5 min and desalting using Micro Bio-Spin P-6 columns (Bio-Rad, 7326221). Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Immunology 2024Quote: ... were covalently coupled to carboxyl-functionalized fluorescent magnetic beads (1-3µg/1.25*10^6 beads) (Luminex Corp. MagPlex® Microspheres; Bio-Rad; Temse, Belgium) employing the BioPlex amine coupling kit (Ref. ...
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bioRxiv - Neuroscience 2020Quote: ... and equal amounts of total protein were loaded onto 4 – 15% Tris-Glycine gels (BioRad). Following electrophoresis (200 V for ∼45 min) ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of cell lysates were resolved by 4–15% precast polyacrylamide gels (Bio-Rad) and transferred to nitrocellulose membrane (0.45μm ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were run in Mini-PROTEAN® TGX 4-15% Precast protein gels (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µg of protein were run through 4-20% Criterion TGX Gels (Bio-Rad 5671093) at 150 V for 185 minutes and transferred to a PVDF membrane using a Trans-Blot Turbo System at 2.5 A for 7 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto a 4–20% Mini-PROTEAN® gradient polyacrylamide gel (Bio-Rad), transferred to PVDF ...
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bioRxiv - Immunology 2021Quote: ... Samples were electrophoresed on a 4-12% TGX stain free gel (Bio-Rad, CA, USA) at 200V for 30-40min ...
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bioRxiv - Genetics 2021Quote: ... Protein fractions were further assessed on TGX stain free 4-20% SDS-PAGE (Biorad, 5678093) and quantified by BCA assay.
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bioRxiv - Neuroscience 2021Quote: Total proteins were separated by electrophoresis on 4%–20% gradient PAGE gels (Bio-Rad, 4561093) and transferred onto nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... and 30 µL of each was loaded into a Criterion TGX 4-15% gel (BioRad, Hercules ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples (10 μg) were separated on 4 % - 15 % Bis-tris gels (Bio-Rad Laboratories) and wet-transferred onto a PVDF membrane ...
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bioRxiv - Pathology 2022Quote: ... Lysates were run on 4-15% Mini-PROTEAN TGX Precast Gels (Bio-Rad, CA, USA) and blotted on 0.2 μm Trans-Blot Turbo Midi Nitrocellulose membrane (BioRad CA ...
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bioRxiv - Microbiology 2022Quote: ... The samples were separated on a 4-20 % gradient Mini Protean TGX acrylamide gel (BioRad), and subsequently transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2022Quote: ... and loaded on 4–15% Mini-PROTEAN®TGX™ precast protein gels (Biorad, 4561083). Proteins were separated by electrophoresis at 70V for 15 minutes followed by 100V for approximately 1 hour using 10x Tris/Glycine/SDS running buffer (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Protein samples were loaded on pre-cast 4-20% TGX polyacrylamide gels (Criterion, Bio-Rad). Electrophoresis was performed in 1x pre-mixed electrophoresis buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: Milk proteins were separated by SDS PAGE on Criterion TGX 4-20% gels (Bio-Rad). Following wet transfer onto a Hybond ECL membrane (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were electrophoresed on 4-20% Mini-PROTEAN TGX Precast gels (BIO-RAD, 456-1094) and transferred to PVDF membranes using a Trans-Blot Turbo Transfer System (BIO-RAD) ...
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bioRxiv - Molecular Biology 2020Quote: Denatured samples were separated on 4-15% Mini-Protean TGX SDS-PAGE gels (Bio-rad). Protein was transferred to methanol-activated PVDF membranes (Bio-rad ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were loaded on 4–20% Mini-PROTEAN TGX Gel (Bio-rad 456-1093) and Western blotting was performed with anti-GFP (Abcam 290 ...
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bioRxiv - Biophysics 2019Quote: ... Protein ubiquitylation was analyzed by SDS-PAGE using 4-15% Mini-PROTEAN-TGX gels (BioRad) and immunoblotting using anti-MBP antibodies.
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bioRxiv - Neuroscience 2019Quote: ... 4×105 cells were resuspended in 400 µl Gene Pulser® Electroporation Buffer Reagent (BioRad) with 23,4 µg of each episomal plasmid (Addgene ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then run on a 4-20% Tris-glycine gel (Bio-RAD, Hercules CA) and transferred onto a nitrocellulose membrane using the Transblot Turbo transfer system (BIO-RAD ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... 15-50 μg of protein was separated on a 4-20% gradient gel (Bio-Rad) and transferred onto 0.2 µm nitrocellulose (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... When required proteins were resolved in 4-20% Mini-PROTEAN® TGX Precast Gel (BioRad) and transferred to nitrocellulose membrane overnight in 48mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... 60 μg of protein lysate was separated by SDS-PAGE (BioRad Miniprotean TGX 4-20%) and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were ran on Mini-Protean TGX 4-20% gradient SDS-PAGE gels (BioRad) and transferred to 0.2 µm PVDF Trans-blot Turbo membranes (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was separated on TGX-stain free pre-cast 4–20% SDS gels (Biorad 4568094) at 200V for 30’ ...
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bioRxiv - Genetics 2020Quote: ... 160 μg of protein was separated on 4-15% Mini-Protean TGX gels (BioRad, Inc.), and then transferred to nitrocellulose ...
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bioRxiv - Systems Biology 2021Quote: ... and samples were separated by SDS-PAGE (Criterion TGX Precast gels, 4-12%, Bio-Rad) under denaturing conditions ...
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bioRxiv - Biochemistry 2021Quote: ... and run on SDS-PAGE (protean TGX pre-casted 4–20% gradient gel; Bio-rad) and transferred to the nitro-cellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: Equal amounts of E11 brain homogenates were loaded onto 4-15% TGX precast gels (BioRad) and transferred to nitrocellulose membranes (or polyvinylidene difluoride for alpha-catenin detection) ...
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bioRxiv - Bioengineering 2021Quote: Samples and controls were loaded on and separated with Stain free 4-20% gels (Biorad). Proteins were transferred onto 0.45 micron PVDF membranes (Bio-rad ...
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bioRxiv - Bioengineering 2019Quote: ... Then 5-10ug protein per sample was loaded onto 4%-20% gradient gel (Bio-rad) and the gel was run for 2 hours at 90mV ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-linked proteins were separated on 4-20% TGX gradient SDS-PAGE gels (Bio-Rad), stained with MS-safe AcquaStain (Bulldog Bio) ...