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Citations for Bio-Rad :
201 - 250 of 456 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse Transcription – Quantitative Polymerase Chain Reaction (RT-qPCR) reactions were performed using SsoAdvanced™ universal SYBR® green (Bio-Rad, Hercules, CA, USA) and run on the CFX Connect (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time polymerase chain reaction for all targets was performed on the CFX-96 Real Time System using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA). Products were confirmed by gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: Relative genetic expression was determined using Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) (CFX96™ Real-Time System, Bio-Rad, CA, USA) using commercially available TaqMan primers (Tables 1-3 ...
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bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative real time polymerase chain reaction (qPCR) was performed using the iQ™ SYBR® Green Supermix (170888, Bio-Rad, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... We optimized the recommended polymerase chain reactions (PCR) cycling conditions and performed a touchdown PCR in a T100 thermocycler (Bio-Rad, Hercules, CA). The total reaction volume was 50 µl which contained 2 µl of genomic DNA at 50 ± 25 ng/µl ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... monoclonal rat anti-human CD3 (Bio-Rad), rat anti-mouse B220/CD45R (clone RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... goat-anti-human 1:3000 (Biorad /1721050); goat-anti-rabbit 1:2000 (CST / 7074) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the light signal was detected and analyzed using a ChemiDoc MP and Image Lab Ver 6.0 (Bio-Rad). List of all antibodies used with dilutions is included in Supplementary Table 2.
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bioRxiv - Neuroscience 2023Quote: ... whose light emission is captured by the camera of the device ChemiDoc Touch (Bio-Rad, Marnes-la-Coquette, France). Quantification of relative protein expression was performed with Image Lab 6.0 software (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... PCR results were visualized using UV light under a gel documentation system (Bio-Rad Gel Doc-XR+; Bio-Rad).
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bioRxiv - Plant Biology 2024Quote: ... PCR results were visualized using UV light under a gel documentation system (Bio-Rad Gel Doc-XR+; Bio-Rad).
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bioRxiv - Evolutionary Biology 2024Quote: ... The electrophoresis results were visualized under UV light using a Bio-Rad ChemiDoc™ XRS+ (Bio-Rad, CA, USA) imaging platform and ImageLab™ (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The electrophoresis results were visualized under UV light using a Bio-Rad ChemiDoc™ XRS+ (Bio-Rad, CA, USA) imaging platform and ImageLab™ (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... were removed from the eluted samples post-purification and quantified using quantitative Real Time polymerase chain reaction using the Bio-Rad CFX 96™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and Promega Plexor®HY Quantitation System (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... IL-17A titres in human serum samples were quantified using a multiplex cytokine panel (Bio-Plex Pro Human Cytokine Assay, BioRad) and a LuminexCorp Luminex 100 machine as per the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The PCR products were run in a 1.5% agarose electrophoresis gel before imaging under UV light using a Gel Doc XR+ Imager (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Image of the gel was captured under UV light using a gel documentation system (Geldoc Go imaging system, Bio-Rad).
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... the two subunits 60S and 40S as well as the very light mRNPs were detected by UV absorbance at 256 nm (BioRad) and each gradient was collected in 10 fractions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed once with PBS and then crosslinked on ice using 0.8 J/cm2 of 254 nm UV light in a GS Gene Linker (Bio-Rad). Cells were then lysed on the tissue culture plate by adding 1 ml of RAP lysis buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... the Stain-Free visualization was enabled by activation of the gels for 45 seconds with UV-light before the proteins were transferred by Trans-Blot Turbo Transfer (Biorad) onto a nitrocellulose membrane (Trans-Blot Turbo RTA ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...
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bioRxiv - Microbiology 2019Quote: ... anti–human IgG conjugated with HRP (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... anti-human IgG-HRP (Bio-Rad 172-1033) and anti-Rat IgG-HRP (Thermo Fisher 31470).
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bioRxiv - Cancer Biology 2023Quote: ... Human GAD1 PrimePCR primers were purchased from BioRad and the sequence for human GAPDH primers are as follows ...
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bioRxiv - Neuroscience 2024Quote: The human cytokine Luminex 8-plex assay (Biorad) was utilised to measure the concentration of IFNγ ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Physiology 2022Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR) was executed with 13 ng cDNA using SSO advanced SYBR Green supermix (Bio-Rad Laboratories, Hercules, CA, USA) with the following settings ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded mature FAM237A was eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Microbiology 2023Quote: ... Ni-NTA agarose with the bound His-SUMO-SigN was loaded on a Poly-Prep® Chromatography Column (Bio-Rad), washed with P2 buffer and subsequently with the P2 buffer with the 30 mM imidazole ...
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bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...