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Citations for Bio-Rad :
2401 - 2450 of 9412 citations for Recombinant Human FGF12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Plant Biology 2021Quote: ... The total protein concentration of each sample was calculated by a Bradford assay following the Bio-Rad Protein Assay protocol (Bio-Rad, USA), and protein concentrations adjusted ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were analyzed on 10% Tris-Tricine SDS-PAGE system (38) with Precision plus protein standard (Bio-Rad 1610363) as a molecular weight marker ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...
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bioRxiv - Biochemistry 2021Quote: ... Harvested cells were lysed in TNI lysis buffer and 10 μg of protein (as measured using Bio-Rad protein assay dye reagent) was aliquoted and precipitated via methanol/chloroform precipitation as described below ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed and homogenized as described elsewhere.30 Protein concentration was determined using a DC protein assay kit (Bio-Rad, 5000116). 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... supernatants were transferred to new Eppendorf tubes and protein concentrations were then determined using a DC protein assay (Bio-Rad, Hercules, CA). 750 µg of protein was diluted in 4X Laemmli sample buffer (LSB ...
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bioRxiv - Molecular Biology 2019Quote: ... The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia™ protein purification system (Bio-Rad) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Protein concentration was measured using the RC DC (reducing agent and detergent compatible) Protein Assay Kit II (5000122, Bio-Rad; Hercules, CA). For qPCR experiments ...
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bioRxiv - Bioengineering 2019Quote: ... For equal loading the protein concentration of the supernatant was determined with BioRad DC protein assay kit II (BioRad#5000116, Cressier, Switzerland) and standard curve with Albumin (ThermoFisher #23210 ...
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bioRxiv - Plant Biology 2020Quote: ... This step was repeated two times and the protein concentration was determined by the DC Protein Assay Kit (Bio-Rad, Munich, Germany) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... Protein concentration was determined in triplicate using a Bradford assay (Bio-Rad protein assay dye reagent concentrate, Bio-Rad Laboratories, Hercules, CA), against bovine serum albumin standards (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... followed a method adapted from our previous research.38 Protein concentrations were determined using the DC Protein Assay (Bio-Rad, Hercules, CA).
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bioRxiv - Plant Biology 2021Quote: ... Plant leaf protein samples (30-100 µg) were run on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Physiology 2019Quote: ... 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044, Criterion™ TGX Stain-Free™ Protein Gel, Biorad) and blotted on PVDF membranes (Immobilon-FL IPFL00010 ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined in triplicate using a commercial colorimetric assay (Bio-Rad Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia). Nuclear and cytosolic fraction enrichment was confirmed by blotting the separated fractions against a nuclear (histone H3 ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Genomics 2021Quote: ... Both total and mitochondrial protein concentrations were determined using the Bradford method (Bio-Rad protein assay, Bio-Rad Laboratories GmbH, München, Germany). Protein extract volumes equivalent to 50–75 μg of total protein were concentrated into smaller volumes by lyophilizing the samples with a Savant Speed Vac Plus SC210A centrifugal evaporator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was transferred to a new tube and total protein concentration was determined by Bradford protein assay (BioRad, C500-0205). For each condition ...
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bioRxiv - Cancer Biology 2021Quote: Fifteen µg of protein per sample was separated on a BioRad Mini-protein 4-15% precast 12 well 20 µl gels (4561085, Bio-Rad, CA) and electrophoretically transferred to Odyssey Nitrocellulose membrane (P/N 926-31092 ...
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bioRxiv - Microbiology 2021Quote: ... protein pellets were resuspended in 100 µL of 6M urea in 50mM ammonium bicarbonate for DC® protein assay (Bio-Rad Laboratories) and trypsin digestion.
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bioRxiv - Neuroscience 2021Quote: ... An aliquot was taken to measure radioactivity and protein content using a liquid scintillation counter and a DC protein assay kit (Bio-rad, USA), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Immunology 2022Quote: ... The protein was concentrated using a 0.5 Centrifugal Filter Unit (Amicon) and then run on a Precast Protein Gel (Bio-Rad, 456-1103) (98 ...
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bioRxiv - Immunology 2022Quote: ... and the amount of protein was quantified by Bradford assay [31] using a colorimetric Protein Assay Dye (500-0006, Bio-Rad, USA) and Bovine Gama Globulin in known concentration for the standard curve ...
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bioRxiv - Immunology 2022Quote: ... and equal amounts of protein were loaded into a 4-15% gradient polyacrylamide gel (BioRad TGX™ Precast Midi Protein Gel #5671085) and proteins separated based on molecular weight using electrophoresis at 110V ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded and electrophorized with a standard of 5□μl of Precision Plus Protein™ All Blue Prestained Protein Standards (BioRad #1610373). For electrophoresis ...
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bioRxiv - Plant Biology 2024Quote: ... The protein concentration of the filtered lysate was determined using the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad; www.bio-rad.com) and bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were separated on Any kD Mini-PROTEAN TGX Precast Protein Gels in a mini electrophoresis system (Bio-Rad Laboratories, California, USA). For molecular weight sizing ...
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bioRxiv - Molecular Biology 2024Quote: ... in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific. Proteins were transferred to a 0.2 µm nitrocellulose membrane by Trans-Blot Turbo™ Transfer system (Bio-Rad). Membranes were washed with TBS (50mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... Histone samples (4 μg protein/well) or whole cell lysates (see above, 40 μg protein/well) were separated on precast gels (4-20%, BioRad, CA, USA) and transferred to 0.2 μm nitrocellulose membranes (Turbo transfer pack ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Bioengineering 2024Quote: Protein expression was confirmed by SDS-PAGE analysis using 10% precast gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Cat# 4561033). The gels were run for 55-minutes at 150V and stained with G-250 Coomassie stain (Bio-Rad ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Equal amounts of protein (15ug) were loaded into each well of a Criterion XT Bis-Tris Protein Gel (Bio-Rad, Hercules, CA). Following gel electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... The reaction rate and total protein concentration (determined by the method of Bradford with the BioRad Protein Assay reagent; BioRad, Hercules, CA) were used to determine CAT activity ...
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bioRxiv - Synthetic Biology 2023Quote: ... prestained protein ladder were applied to SDS-PAGE using 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad, Hercules, CA, USA). Western blots were carried out in a Trans-Blot Turbo system using Trans-Blot Turbo Mini 0.2 μm Transfer-Packs (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was kept and the total protein concentration was determined by standard Bradford method (Bio-Rad Protein Assay Dye Reagent #5000006) using a BSA calibration curve (MP BIomedicals #160069 ...
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bioRxiv - Genetics 2023Quote: ... 50 µg of whole protein lysate was used for all immunoblots and resolved on 4-20% Mini-PROTEAN TGX Precast Protein gels (BioRad; Cat# 4561094). Protein samples were transferred to 0.2 µm PDVF membranes (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... and cell pellets were lysed in 1× Laemmli buffer before proteins were separated on 4-20% Mini- PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and transferred to a nitrocellulose membrane (Odyssey® nitrocellulose membrane ...
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bioRxiv - Biochemistry 2023Quote: ... ATP contents were expressed in nmol/mg protein (using the Bio-Rad Protein Assay Cat. #500-0006, Bio-Rad, Hercules, California, USA).
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extracts were sonicated and cleared by centrifugation and protein concentration estimated using the Bio-Rad DC protein assay kit (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... The spun down lysates were measured for total protein using a BCA assay and 15 μg of protein was diluted in 2x Laemmli buffer containing β-mercapto ethanol (Bio-Rad) and heated to 95 °C for 5 mins ...