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Citations for Bio-Rad :
2401 - 2450 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... 72°C for 30 seconds and a final elongation step at 72°C for 1 minute on Bio-Rad T100 Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). PCR products were cleaned using an ExoAP reaction (see Supplemental Methods ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 72°C for 3:40 min and a final elongation step at 72°C for 1 minute on Bio-Rad T100 Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). Amplicons were run on a 1% agarose gel and the highest molecular weight fragments (> 5kb ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Microbiology 2022Quote: ... and Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, California, USA) were used to detect urinary cytokines ...
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bioRxiv - Microbiology 2022Quote: ... and a goat anti-human IgG conjugated to HRP (Bio-Rad, cat. 204005) was used as secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were analyzed using a human Th17 cell cytokine panel multiplex (BioRad 171AA001M). For flow cytometry ...
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bioRxiv - Bioengineering 2020Quote: ... or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1) antibodies (clone H398, Bio-Rad). DAPI was used for dead cell exclusion.
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bioRxiv - Immunology 2023Quote: ... and Bio-Plex Pro Human IgA detection antibody (Bio-Rad, Cat No. 12014669). For both kits ...
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bioRxiv - Pathology 2023Quote: ... Immunoglobulins were analysed using a bio-plex pro human isotyping assay (Bio-Rad) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and Bio-Plex Pro Human Cytokine Screening Panel Standards (BIO-RAD, Hercules, CA). Cytokine and chemokine concentrations refer to 8-point standard curves.
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bioRxiv - Biophysics 2019Quote: ... The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad, Hercules, California). In all purification steps ...
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bioRxiv - Physiology 2019Quote: ... Gels were run at 150 V for 1 hour and the protein were subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad, Hercules, CA, USA). All membranes were stained with Ponceau as a qualitative check for equivalent of total protein loading and transfer ...
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bioRxiv - Neuroscience 2019Quote: ... or POPC:palmitoyloleoylphosphatidylserine (POPS (85:15) at a 1:200 protein to lipid ratio by dialysis in the presence of Bio-Beads (Bio-Rad, Hercules, CA) into metal free buffer ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were boiled 1 min and subjected to SDS-PAGE using a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel (Bio-Rad Ref. 4568085). After SDS-PAGE ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification conditions were initial denaturation at 95°C for 10 min followed by 40 cycles of 95°C for 10 s and annealing at 58-65 °C for 30 s (Bio-Rad CFX Connect Real-Time PCR) followed by melt peak analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then sonicated and their protein content was quantified using the Bradford protein assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufactureŕs instruction and measured using the EonTM High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Each gel was loaded with a Precision Plus Protein™ Dual Color Standards as a protein marker (Bio-Rad, 1610374). Gel electrophoresis was stopped when the Bromophenol blue dye reached the bottom of the gel ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Genomics 2019Quote: ... media were collected for insulin determination and rat islet cells were lysed with acid-ethanol (0.2 mM HCl in 75% ethanol) to extract total insulin content or with protein lysis buffer to measure total protein content (Bradford, BioRad). The amount of insulin released in the medium and remaining in the cells was measured by insulin Elisa kit (Mercodia) ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant representing the nuclear fraction was collected and protein concentration of both fractions was determined using the DC protein assay (Biorad). Cytosolic and nuclear fractions were further diluted to the desired protein concentration using the corresponding lysis buffers ...
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bioRxiv - Cancer Biology 2019Quote: ... The volume of the target protein bands and total protein volume of each lane were normalized and quantified using ImageLab software (Biorad). For all figures shown ...
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bioRxiv - Plant Biology 2019Quote: ... The amount of protein recovered after lysis was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad), with bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Trehalase activity was standardized to total protein content using the DC protein quantification technique according to the manufacturer’s recommendations (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... The quantification of protein content in exosome suspensions and whole-cell lysates was undertaken by a modified Lowry assay (DC Protein Assay, BIORAD). For immunoblotting ...
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bioRxiv - Physiology 2021Quote: Equal amounts of skeletal muscle protein extract (45µg) were separated on Mini-Protean TGX precast protein gels (Biorad, CA, USA) and transferred to a PVDF membrane using a Trans-Blot semi-dry transfer system (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... AS and EVs were lysed in RIPA buffer and protein concentration was measured with DC Protein Assay (Biorad, 500-0116), using BSA as standard ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatants were collected and protein concentration was determined using the Bradford-based Bio-Rad protein assay (Bio-Rad). Samples were then denatured and separated using SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted in SDS page loading buffer and separated on 4-15% Mini-PROTEAN TGXTM Precast Protein Gels (BioRad). Samples were processed for immunoblotting or fixed and stained for mass spectrometry using Imperial protein stain (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... The amounts of isolated mitochondria were expressed as the protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Normalization among different technical replicates and conditions was carried out by normalizing the relative luminescence units (RLUs) against total protein content (calculated with the Bradford protein assay, BioRad). Each condition was measured in quadruplicates and each experiment was performed at least twice.
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bioRxiv - Biochemistry 2022Quote: ... The protein content of the LDL sample was determined by a protein assay kit (Bio-Rad Laboratories, Inc., Tokyo, Japan) based on the Bradford method using bovine serum albumin as a standard ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were separated by precast 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad Laboratories 5678085), transferred to a nitrocellulose membrane using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad Laboratories 1704159EDU ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... 1x PIC) using a sonifier BRANSON 250.The protein in crude homogenates was determined by Bradford protein assay (BioRad, USA) and 50 μg of protein was used for a subsequent chloroform/methanol extraction ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble extract was collected and total protein concentration determined using Protein Assay Dye Reagent Concentrate (BIO-RAD, Cat No 5000006) with BSA as standard ...