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Citations for Bio-Rad :
2401 - 2450 of 9902 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the Bio-Rad protein assay according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA). The proteins were then separated on a 10% polyacrylamide-SDS gel (90 µg of protein/lane ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 30 to 40 µg of protein were loaded into 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, 4561084), electrophoresed between 125 and 200 V for 30 min and wet-transferred to PVDF membranes (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracted from the samples were quantified using RC DC Protein Assay Reagents Package (Bio-Rad Laboratories Ltd, Cat. No. 5000120,).
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bioRxiv - Neuroscience 2024Quote: ... SDS-PAGE-separated proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Immun-Blot® PVDF Membranes for Protein Blotting, Bio-Rad) with a transfer buffer (25 mM Tris-base ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration in the homogenates was determined using a commercial assay kit (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA, USA). Na+/K+-ATPase and H+-ATPase activities were expressed as µmol ADP mg protein-1 h-1 and carbonic anhydrase activity was expressed as µmol H+ mg protein-1 min-1.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were boiled at 95°C for 5 min and proteins were separated on 4–15% Mini-Protean TGX precast protein gel (Bio-Rad) and transferred onto hydrophobic polyvinylidene fluoride (0.45 μm PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... and Western blots were performed using standard molecular biology procedures Proteins were resolved by 4%–20% Criterion TGX protein gels (Bio-Rad) and transferred to PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... and 20 μg of proteins were separated by SDS-PAGE using 4-20% Criterion TGX Stain-Free Protein Gels (5678094, Bio-Rad). Total protein stains were activated by UV excitation using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the samples were analyzed by western blotting using equal protein amounts of cell lysates separated by SDS–PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The same total protein amounts were loaded into each lane of 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Completed gels were transferred onto 0.2 um nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting supernatants were retained and protein concentrations were determined using the DC Protein Assay Kit II (Bio-Rad, Feldkirchen, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Samples (30 μg per sample) were loaded in TGX Stain-Free Protein Gels with one lane dedicated to a protein standard (Bio-Rad) and run at 100 V ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the PierceTM BCA protein assay kit and diluted with the 4x Laemmli sample buffer (BioRad, 1610747) before denaturation at 95°C for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... The proteins were separated by SDS-polyacrylamide gel electrophoresis (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, BIO-RAD), transferred to nitrocellulose ...
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bioRxiv - Developmental Biology 2024Quote: ... and images of both the target proteins and the total protein were analyzed using ImageLab software (version 6.0.0, Bio-Rad, Hercules, USA) to determine the intensity per mm² of each band and lane ...
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bioRxiv - Genetics 2024Quote: We loaded 10µL of protein sample per well on the Any kD Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad Laboratories). We ran the gel for 90 minutes at 100V in Tris/Glycine/SDS buffer ...
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bioRxiv - Biophysics 2020Quote: ... 3 mL of acrylamide/bis solutions (40%, Bio-Rad Laboratories, CA, USA), 1 mL of 10X tris-borate-EDTA (TBE ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 trans-blot papers (Bio-Dot SF Filter Paper, Bio-Rad, #1620161) and one cellulose acetate membrane (Cellulose Acetate Membrane Filters ...
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bioRxiv - Molecular Biology 2023Quote: ... to 3 mL for buffer exchange with an Econo10 DG column (Biorad) equilibrated in buffer A and eluted with 4 mL of the same buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... using a Mini PREOTEAN 3 cell (525BR058974; Bio-Rad, Hercules, CA, USA) and PowerPac™ Power Supply (043BR09142 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times with 0.1% Tween-20 (Bio-Rad,1706531) TBS (TBTS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... were loaded onto 3-8% Criterion XT tris-acetate gels (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-F4/80 FITC (3:100, Bio-Rad Laboratories, Temse, Belgium). The single cells were washed with 1XPBS+1%FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and then washed again 3 times before revelation by electrochemoluminescence (Bio-Rad) using the Chemi-doc XRS+ (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... After 3 washes with 0.05% Tween 20 (catalog number 1706531, Bio-Rad) in PBS (TPBS) ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was blocked by dipping in 3% skimmed milk (Bio-Rad) in PBS containing 0.1% Tween 20 (Amresco ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were loaded on a 3-8% tris-acetate gel (Biorad 3450131) in Tricine running buffer (Biorad 1610790 ...
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bioRxiv - Immunology 2024Quote: ... and 3 μL of 10 % (w/v) ammonium persulfate (#1610700, Bio-rad) were added to 500 μL aliquots ...
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bioRxiv - Neuroscience 2024Quote: ... Each fraction is run through a 3-15% gradient gel (BioRad 45610840) alongside full keratinocyte cell lysate (+ ...
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bioRxiv - Cancer Biology 2024Quote: ... and filtered 3 times through gel filtration biospin P30 (Bio-Rad France). The purified NAMPT was then transferred to NAMPT elisa wells for quantitation as described in experimental section ...
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bioRxiv - Biophysics 2024Quote: ... and 3 μL of N,N,N°,N°-tetramethyl ethylenediamine (Bio-Rad) were added to initiate the acrylamide polymerization ...
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bioRxiv - Genetics 2021Quote: ... assessment of protein concentration using Pierce™ BCA Protein Assay Kit (Thermo cat#23225) and addition of 4x Laemmli Sample buffer (Bio-Rad). 40ng of each sample was loaded onto Mini-PROTEAN GTX Precast Protein Gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein concentration was determined by the Bradford method using the Bio-Rad Protein Assay Kit II (Bio-Rad, 5000 002) and serial dilutions of bovine serum albumin as a calibration standard (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... Molecular weight changes of the antigen were evaluated by reducing SDS-PAGE and protein bands were visualized with high sensitivity Flamingo Fluorescent Protein Gel Stain (1610491, Bio-Rad Laboratories). Intact antigen fractions were determined by digital imaging of gels followed by ImageJ analysis ...
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bioRxiv - Immunology 2021Quote: ... These tubes were then centrifuged at 300G for 5min at 4C and the supernatants were used for protein quantification using the DC protein quantification assay protocol (Bio-rad 5000112). After incubation at −80 °C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were centrifuged in v-bottom 96-well plates rather than microcentrifuge tubes and total protein concentration was determined using Protein Assay Dye (BioRad #500-0006) following standard protocols ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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bioRxiv - Bioengineering 2021Quote: ... the homogenate was centrifuged at 16,000g for 5 min and 5 µl of the supernatant was used for protein measurement in a microplate format utilizing the Lowry DC protein assay kit and γ-globulin for generating standard curve (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of the supernatants was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-rad Laboratories, Inc., CA, USA); the absorbance was measured at 595 nm with a Thermo Scientific Multiscan® GO (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatants were transferred into a new set of tubes and protein concentration was determined using Bio-Rad DC™ protein assay kit (Bio-Rad). Western blot protocol is the same as described before ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad, 5000111) using a photospectrometer at 750 nm (Molecular Devices ...
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bioRxiv - Plant Biology 2021Quote: ... The total protein concentration of each sample was calculated by a Bradford assay following the Bio-Rad Protein Assay protocol (Bio-Rad, USA), and protein concentrations adjusted ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein-containing lysates of exosomes (5 μg) were run on a 4–20% Mini-PROTEIN TGX gel (Bio-Rad, Hercules, California, USA) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Equal amounts of protein were analyzed on 10% Tris-Tricine SDS-PAGE system (38) with Precision plus protein standard (Bio-Rad 1610363) as a molecular weight marker ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble protein content was spectrophotometrically determined using bovine serum albumin as a standard (Bradford Protein Assay Kit; Bio-Rad, Hercules, CA).
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bioRxiv - Biochemistry 2022Quote: ... The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Biorad). Fractions containing high molecular weight species were pooled together and concentrated to <500 µL using a 100-kDa cutoff concentrator ...
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bioRxiv - Cell Biology 2022Quote: ... This step was repeated two times and the protein concentration was determined by the RC DC Protein Assay Kit II (Bio-Rad, 5000122). Two times Laemmli buffer was added ...