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Citations for Bio-Rad :
2401 - 2450 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... proteins were subjected to primary antibody recognition using an anti-P1 antibody raised in rabbit (Siré et al, 2008) and purified by Affi-Gel 15 immnunoaffinity chromatography (BIO-RAD) using the recombinant P1 protein as the ligand (used at a 1:1000 dilution) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein bands were visualized via enhanced chemiluminescence (Pierce ECL Western blotting substrate) using horseradish peroxidase-conjugated secondary antibodies and imaged using the ChemiDoc system (Bio-Rad). The quantifcation of the band was performed with ImageLab software (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was incubated with secondary HRP-conjugated antibodies and signals were detected using a chemiluminescent method (ChemiDoc XRS, Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... 1:5000), or donkey anti-goat antibody (sc-2020, 1:5000) and signal was detected with enhanced chemiluminescence substrate (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... were purchased from Thermo Fisher Scientific while secondary antibodies Goat anti-mouse HRP (172-1011) and Goat anti-rabbit HRP (170-8241) were from Bio-Rad. MG132 (C2211 ...
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bioRxiv - Immunology 2019Quote: ... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated with secondary antibodies for one hour at room temperature and developed using Clarity ECL Detection Kit (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... 1:1000)The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1hr and the detection was performed using the immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were chosen according to the species of origin of the primary antibodies and detected by an enhanced chemiluminescence system (Bio-Rad). Densitometric analysis of the relative expression of the protein of interest versus the corresponding control (β-actin or total STAT3 ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were probed with appropriate secondary antibodies conjugated with HRP and imaged with a ChemiDoc XRS digital imaging system (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... After four washing steps with PBST the respective secondary antibodies were added for 1 h at RT: goat anti-mouse-HRP conjugate (Bio-Rad) at 1:3000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary and secondary antibodies were used as per the manufacturers’ instructions and membranes were imaged on the ChemiDoc MP (Bio-Rad) after incubation in Clarity Western ECL Substrate (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... 7 μm cryosections were methanol fixed for 20 minutes on ice and stained with an antibody specific to CD68 (clone: ED1, Bio-Rad). Animal protocols were approved by the University of Virginia Institutional Animal Care and Use Committee.
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bioRxiv - Biophysics 2019Quote: ... was used and the signal from HRP-conjugated secondary antibodies was detected with an ECL system and an enhanced chemiluminescence system (ChemiDoc Touch System, Bio-Rad). Quantification of the mean band intensity relative to the loading control Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2019Quote: ... and lysed then immunoprecipitated as described above using homemade rabbit anti-PGRN antibodies bound to Affi-Gel 15 (Bio-Rad Laboratories). Samples were then mixed and boiled 5 minutes with 10mM DTT followed by alkylation by treating samples with a final concentration of 28 mM iodoacetamide ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with a secondary antibody and 1 × TBST washes the membrane was coated with chemiluminescent substrate (Clarity Max ECL substrate, Bio-Rad) and imaged on Chemidoc MP (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Biochemistry 2020Quote: ... for 1hr at RT and probed with HRP-conjugated anti-rabbit or mouse IgG antibodies and detected with ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... the membrane was incubated with an anti-rabbit or anti-mouse IgG antibody conjugated with horse radish peroxidase (HRP) (BioRad Laboratories) and developed with Clarity Western ECL Substrate (BioRad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated for mouse (CST 7076) or rabbit (CST 7074) were used with Clarity-ECL chemiluminescence reagent (Bio-Rad) for detection ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen-primary antibodies complexes were detected with secondary antibodies conjugated to horse radish peroxidase and developed using a chemiluminescent reagent (Clarity ECL, Bio-Rad). Images of the WBs were acquired using a ChemiDoc MP System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were washed five times with TBST and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (adsorbed with mouse and bovine to prevent cross-reactivity, 1:10,000, #STAR124P, Bio-Rad Laboratories) in TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... incubated with specific primary antibodies for 1 h and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies by using ClarityTM Western ECL Blotting Substrate (Bio-Rad). For pulldown assays between cell extract and purified Nyx and MAG mutants ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... The blots were probed for 1 hour at room temperature with α-CaHsp9031 diluted 1:10,000 in PBST with 0.1% milk and washed with 1x PBST prior to probing with α-rabbit antibody-HRP conjugate (BioRad #1705046) for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... the anti-rabbit secondary antibody was incubated for 1 h and chemiluminescence was measured using a ChemiDoc XRS+ imaging system (Bio-Rad) after development in chemiluminescence reagent (Westar Nova 2011 ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution in PBS with 1% (w/v) skimmed milk and 0.1% (v/v) Tween-20 or goat anti-porcine IgA HRP (Bio-Rad Antibodies) at 1:20,000 dilution in the same diluent.
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bioRxiv - Immunology 2021Quote: ... The primary antibody was removed the next day and the membrane was incubated with secondary antibody at room temperature for 1 h. Enhanced chemiluminescence substrate (Clarity Western ECL Substrate, Catalog no. 1705060) from Bio-Rad Laboratories (Mississauga ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity-ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates of WT OSC were incubated with 1μg of Rabbit anti-Piwi polyclonal antibody (18) and 30 μl Surebeads Protein A Magnetic beads (Bio-Rad, 1614013). Immunoprecipitation was performed at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: Western blot detection was performed by enhanced chemiluminescence using HRP-coupled secondary antibodies and the Clarity or Clarity Max substrates (Bio-Rad). The signal was digitized using Amersham 600RGB imager as 16-bit grayscale TIFF files ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... The membrane was incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies in 5% milk and bands were developed with Chemi-Doc XRS imaging (Bio-Rad). The primary antibodies used are listed in Supplementary Table 3.
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bioRxiv - Bioengineering 2022Quote: ... Antibody in the hybridoma supernatant was purified by passing through a column of DEAE Affigel blue (Bio-Rad, Sydney, NSW, Australia), dialysed into a Tris-saline buffer (Tris 10 mM ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies were applied at room temperature for one hour and the blot was visualized using a ChemiDoc MP System (Bio-Rad). RBM20 bands were quantified with Image Lab software and normalized to the γ-tubulin loading control.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse secondary antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and then incubated with the secondary antibody (Goat Anti-Rabbit IgG – HRP conjugate, Bio-Rad cat# 1706515) for one hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies and detection using Clarity Western enhanced chemiluminescence substrate (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with appropriate secondary antibodies before developing with Perkin-Elmer ECL Western Lightning Plus solution and visualized using a Chemidoc imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed thrice and incubated with a secondary antibody (Goat anti-Mouse IgG-HRP, 1706516, Bio-Rad Laboratories, 1:10K), also diluted in 2.5% milk in TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... and followed by one-hour incubation with diluted secondary antibody (1706515 Goat Anti-Rabbit IgG-HRP Conjugate, Bio-Rad, Hercules, CA). Protein lysates from each cell line were analysed on multiple gels (four per cell line ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...