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Citations for Bio-Rad :
2351 - 2400 of 6899 citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The obtained DNA extract (100 μL) was stored at −20 °C until further investigation and subjected to qPCR analysis using 96-well plates (Bio-Rad, Hercules, USA), the Thermocycler CFX connect system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad, Hercules, CA). The result is the representative from one individual’s CD14+ samples and a total of 2 healthy individuals’ CD14+ monocytes were tested ...
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bioRxiv - Neuroscience 2022Quote: ... The embryoid media was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Anti-Anti, 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was carried out in 96-well plates using a CFX-96 cycler equipped with CFX Maestro software (Bio-Rad, Hercules, CA, USA). The thermocycling conditions were as follows ...
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bioRxiv - Genomics 2019Quote: ... We carried out real time PCR reaction and analyses in 384-well optical reaction plates using the CFX384 instrument (Bio-Rad, Hercules, CA).
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bioRxiv - Pathology 2020Quote: ... Real-time RT-PCRs were carried out in 96-well plates on a Bio-Rad CFX96™ Real-Time PCR system (Bio-Rad Laboratories,) or a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... We then prepared eight four-fold serial dilutions of this cytokine/chemokine protein standard pool and transferred 50 µL of each dilution to a 96-well Bio-Plex Pro™ Flat bottom plate (BioRad, Catalog# 171025001). Final standard curves were optimized and generated using Bio-Plex Manager 6.1 software ...
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bioRxiv - Microbiology 2021Quote: ... 12 – 16 individual colonies from each condition were selected from the m-TSAYE plates and DNA was extracted using Bio-Rad InstaGeneTM matrix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol for DNA extraction from bacterial cells ...
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bioRxiv - Immunology 2021Quote: ... according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad, Hercules, CA). Samples were diluted to 1:3 for PEG− 2 and LTB4 analysis and 1:20 for IL-8 measurements.
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bioRxiv - Microbiology 2021Quote: All melt-MAMA PCRs were performed on a CFX96 Real-Time PCR Detection System using white hard shell 96-well PCR plates or 0.2 mL 8-tube PCR strips (Bio-Rad, Gladesville, NSW, Australia), and PCR results examined using the CFX Maestro v4.1.2433.1219 software (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 100µl of 1xPBS was added to each well and the plate was covered with a foil seal (catalog# MSF-1001, Bio-Rad, Hercules, CA). Images were taken on an InCell 6000(GE Healthcare Bio-Sciences ...
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the plates were analyzed for luminescence signals using a ChemiDoc Imaging System and Image Lab 6.0.1 software (Bio-Rad Laboratories Inc., Hercules, USA). Exposure time was set to 5 sec ...
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bioRxiv - Cancer Biology 2024Quote: Total protein concentrations were determined against a standard curve of BSA in a 96-well plate using the DC™ Protein assay (Bio-Rad Laboratories). Proteins were separated on reducing 10% NuPAGE™ Novex™ Bis-Tris Protein Gels and transferred onto PVDF membranes ...
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bioRxiv - Developmental Biology 2023Quote: ... The assay was performed in a 96-well plate in a final volume of 200 µL containing the sample and Bradford’s solution (BioRad, Marnes-la-Coquette, France). After a 5-min incubation at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA template were loaded onto a 384-well plate and run through 40 cycles on a CFX384 real time cycler (Bio-Rad Laboratories, Inc). The data were analyzed using the manufacturer’s CFX manager software ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification reactions were conducted in 96-well optical plates on a Bio-Rad CFX Connect using 2x iTaq Universal SYBR Green Supermix (Bio-Rad, California, USA), 0.25 µM of each primer ...
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bioRxiv - Bioengineering 2023Quote: ... Amplification was carried out in 96-well plates using a CFX-96 cycler equipped with CFX Maestro software (Bio-Rad, Hercules, CA, USA). The thermocycling conditions were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA template were loaded onto a 384-well plate and run through 40 cycles on a CFX384 real time cycler (Bio-Rad Laboratories, Inc). The data were analyzed using the manufacturer’s CFX manager software ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB2 was detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted with the Nucleospin RNA Isolation Kit (Machery-Nagel) and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). TaqMan real-time qPCR assays were used for quantification of human IFNB1 (Hs01077958_s1) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted using the PicoPure RNA Isolation kit and cDNA synthesis was performed using the iScript cDNA synthesis kit (Bio-Rad). cDNA samples were amplified with SYBR Select mastermix (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB1 and DdvT were detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced™ - Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated either from homogenized mosquitoes or infected cell supernatant using a Multisource RNA Miniprep Kit (AP-MN-MS-RNA-250, Axygen) and reverse transcribed to cDNAs using an iScript cDNA Synthesis Kit (1708890, Bio-Rad). Viral genomes were quantified by qPCR using iTaq Universal SYBR Green Supermix (1725121 ...
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bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes One-Step kit (Bio-Rad). The 2019-nCoV_N_Positive Control plasmid (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was reversely transcribed with 0.5–1 μg RNA using the iScript cDNA synthesis kit as described in the kit (Biorad, Hercules, CA). The gene expression of Il-6 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted using the RNeasy plus micro RNA extraction kit followed by reverse transcription using iScript cDNA synthesis Kit (Bio-Rad). Equal amounts of DNA and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using the NucleoSpin RNA Plus Kit (Machery-Nagel) followed by reverse-transcription with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1μg of total RNA was reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (iScript cDNA Synthesis Kit, Bio-Rad) according to the provided protocol ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) was synthesized using a Qscript kit (QuantaBio, MA) as per kit instructions in a T-100 (Bio-Rad) thermocycler.
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... from ART or ART + GYY4137 treated groups were harvested to isolate total RNA using Qiagen RNAeasy isolation kit and 200 ng of total RNA was reverse transcribed (iScript™cDNA synthesis kit, Bio-Rad). Reverse transcribed cDNA was diluted 10-fold and amplified using primers against HIV LTRs and seminested – PCR was performed using primers and probe listed in Table S6 ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Physiology 2020Quote: ... RNA of treated cells was isolated using EZ-RNA isolation kit (Biological Industries Beit Haemek, Israel) and cDNA was synthesised using iScript cDNA synthesis kit (Bio-Rad, UK). Real-time qPCR analyses were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... we purified total RNA using the Qiagen RNeasy Mini kit (Cat#74106) and made cDNA using iscript cDNA synthesis kit from Biorad (Cat#1725036), followed by a Taqman assay to detect AHR transcription (Hs00169233-m1 for AHR expression and Hs0442063-g1 for GAPDH ...
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bioRxiv - Biochemistry 2021Quote: RT-qPCR was performed using the 2019-nCoV RUO Kit (Integrated DNA Technologies, #10006713) in combination with the iTaq™ Universal SYBR® Green One-Step Kit (BioRad). Each 20 μl reaction contained 10 μl 2X iTaq universal SYBR® Green reaction mix ...
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bioRxiv - Evolutionary Biology 2021Quote: RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Cat# 74104) and cDNA was prepared using the iScript cDNA Synthesis Kit (Biorad, Cat# 1708890). Assays were done in triplicate using Express SYBR GreenER master mix (Invitrogen ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Whole-larvae cDNA was generated by isolating total RNA from 3–5 dpf zebrafish larvae using the E.Z.N.A Total RNA kit (Omega, Bio-Tek INC, Cat# R6834-01) and reverse transcribing using the iScript cDNA synthesis kit (BioRad, Cat# 1708891). Fragments of coding sequences of these genes were amplified by PCR using gene-specific primers as follows:
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted using a total RNA purification kit (NORGEN, Auburn, WA) followed by cDNA synthesis utilizing iScript cDNA kit (BIORAD, Hercules, CA). Quantitative real time-PCR using iTaq Universal SYBR GREEN Supermix (BIORAD ...
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bioRxiv - Biophysics 2020Quote: The prepared DNA samples (5 μL) were mixed thoroughly with 1 μL of 6X DNA loading buffer (Bio-Rad Laboratories). The mixtures were loaded into the wells of the prepared gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Cell Biology 2019Quote: Polymerization of the soft pre-mix was started immediately after the previous step by addition of 5 µl 10% ammonium persulphate (APS; BioRad) and 1 µl N ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’KI and 3’KI as detailed (Fig 2A and D) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... by Bio-Rad tans-blot turbo transfer system at mixed weight transfer setting and incubated in 5% blotting grade blocker (BioRad Catalog: 1706404) solution for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was obtained by incubating the samples (whole embryos or adult caudal fin fragments) in TE buffer supplemented with 5% Chelex-100 (BioRad) and 10 μg/ml Proteinase K (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... Tween 20 (TBS-T) in 5% (w/v) milk (pH 7.4) followed by incubation with an anti 6xhistidine antibody (Bio-Rad) overnight ...