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Citations for Bio-Rad :
2301 - 2350 of 8940 citations for Recombinant Human TNFRSF17 protein Fc His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Remaining protein was eluted using 2X SDS (Biorad) sample buffer with 100 mM DTT (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Protein was transferred to a PVDF membrane (Biorad) using a Trans Blot Turbo Transfer System ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein was quantified with Bradford reagent (Biorad) and samples containing 30 µg total protein were analyzed by western blotting on 4-12% Bis-Tris Gels (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The development of the colonies of the fluorescently labeled Trichoderma strains was monitored by making regular (every 24 h) images in a Bio-Rad ChemiDoc MP system (Bio-Rad, USA) equipped with multiplex fluorescent channels ...
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bioRxiv - Neuroscience 2021Quote: Five slices were taken from the nucleus accumbens of each animal and labeled with the Helios Gene Gun (Bio-Rad, Hercules, CA). Previously prepared cartridges were delivered at 70 psi through 3 um pore filter papers onto the tissue ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were then separated by 6.5% (w/v) native polyacrylamide gels at 4 °C and the biotin-labeled probes was captured with ChemiDoc™XRS+ (Bio-Rad). The oligonucleotide probes used in this study are listed in Supplemental Data Set S1.
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bioRxiv - Biophysics 2024Quote: ... Labeled RPA variants were separated from excess dye using a Biogel-P4 gel filtration column (Bio-Rad Laboratories; 65 ml bed volume) using storage buffer (30 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor samples were homogenized in RIPA buffer (Thermo, #89901) followed by protein concentration measurement using DC Protein Assay (Bio-Rad, #5000112). Tumor lysates were immunoblotted as above ...
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bioRxiv - Cell Biology 2020Quote: ... Concentration of total cellular protein was measured according to the DC™ protein assay according to the manufacturer’s instruction (BIO-RAD).
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bioRxiv - Microbiology 2020Quote: ... and the total protein concentration of the samples was determined by Bradford assay70 with Protein Assay Dye Reagent Concentrate (BIO-RAD). Samples were normalized by total protein content and run in a 10% SDS-PAGE gel for protein separation ...
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bioRxiv - Cell Biology 2019Quote: Non-transfected and transfected cells were lysed in MNT and the PNS was isolated by centrifugation followed by total protein concentration determination using 595 nm Protein Assay Dye Reagent (Bio-Rad). All subsequent steps were conducted at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... EVs were characterized based on particle size and number using nanoparticle tracking analysis (NS300, Nanosight) and protein concentration (DC protein assay, Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB2 was detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The GlcA-T activity was assessed by subtracting the background observed in the negative control sample without donor sugar and normalized against the amount of protein measured using the DC Protein Assay (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... the volume of BioID lysis buffer added was based on protein concentration determination using the DC Protein Assay Kit from Bio-Rad on the 10% aliquot samples lysed in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged and the protein content of the supernatant was measured using the Protein Assay Dye Reagent (Biorad, #500-0006). Immunoblotting was performed using from 10 to 50 μg of protein lysate ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: Membrane and cytosolic proteins fractions from MS were extracted using ReadyPrep™ Protein Extraction Kit (Membrane I) (Bio-Rad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins and/or immunocomplexes were separated by electrophoresis on a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed with anti-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2019Quote: ... total protein concentration of the supernatant was assayed by using Bio-Rad protein assay kit (Bio-Rad, Marnes-la-Coquette, France). Equal amounts of each protein sample were separated on NuPAGE Bis-Tris or Tris-Acetate gels and transferred to nitrocellulose or PVDF membranes ...
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bioRxiv - Microbiology 2019Quote: ... The amount of protein in the biomass was determined by the Quick Start™ Bradford Protein Assay (BioRad, Mississauga, ON, Canada). The denitrification rates were calculated by the linear portion of the NO3− plus NO2− concentrations (NOx ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 40-60μg of protein was electrophoresed through 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hertfordshire, UK) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB1 and DdvT were detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The purified proteins were separated by SDS/4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA), then transferred to an Immobilon-FL PVDF membrane (Merck Millipore Ltd. ...
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bioRxiv - Bioengineering 2020Quote: ... Equal amounts of protein lysates (10-20 μg total protein) were subjected to 4–20% SDS-PAGE (Bio-Rad, Hercules, CA). Western analysis was carried out with primary antibodies against phosphorylated Smad1/5 (p-Smad1/5) ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of the protein samples were separated on 4-20% SDS-PAGE (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) at 20 mA and 250 V as the limiting voltage.
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bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined using the BioRad DC microplate assay protocol (BioRad DC Protein Assay Kit, BioRad, Hercules, California #5000112). Protein Standards and samples were run on NuPAGE 10% Bis-Tris Gels (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad®) for separation (200 V ...
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bioRxiv - Neuroscience 2021Quote: ... and sonicated (twice; 70 % power for 30 sec, Sonopuls, Bandelin) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... cAMP content in TCA extracts was determined by radioimmunoassay (RIA) (83) and normalized to protein [(determined using a dyebinding protein assay (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... Additional one-ml samples were used for total protein determination using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). β-galactosidase assays were performed as described previously (38) ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μl of brain homogenate from each sample was analyzed for protein concentration using BioRad protein assay reagent (BioRad, Hercules, CA). 1 μl of 0.2 M perchloric acid per sample was added to the remaining homogenate and was centrifuged at 10,000 rpm for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were normalised after Bradford protein quantification and resolved on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad), transferred to nitrocellulose and blocked in 5% milk in PBS-T (PBS + 0.05% Tween 20) ...
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bioRxiv - Physiology 2020Quote: ... 15 μg of protein was separated on a 10% Criterion® TGX™ Precast Protein Gel (BioRad Laboratories, 56671034, Hercules, CA) and transferred to a nitrocellulose membrane using a Trans-Blot TurboTransfer System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2019Quote: Protein concentrations in the EVs and WCP samples were determined by Quick StartTM Bradford protein microplate standard assay (Bio-Rad, USA). For protein separation ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled for 5 min and the proteins were separated in 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-RAD) and electroblotted to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration of the EV was determined using the Quick Start™ Bradford protein assay according to manufacturer’s guidelines (BioRad, UK). In short ...
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bioRxiv - Neuroscience 2020Quote: ... 40 μg protein of N13 cell lysates was loaded to 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BioRad) and transferred to nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Transfer Kit (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of the protein extracts were loaded on a 4-12% gradient 26 well Criterion XT Bis-Tris Protein gel (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... steroid concentrations or their ratios were normalized to the total protein content of the incubations as determined in the supernatant after cell lysis using the DC Protein Assay (Bio-Rad).
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and the protein concentration was measured using a DC protein assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of protein (5 μg ...
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bioRxiv - Biochemistry 2021Quote: ... 10 uL of the protein loading mixture was loaded into the wells of either 12% mini-PROTEAN TGX precast protein gels (Bio-rad) or 12.5% SuperSep Phos-tag precast gels (Wako Pure Chemical Industries) ...
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bioRxiv - Biophysics 2019Quote: ... Gradients were manually fractionated into 200 μL fractions and protein concentrations were determined by the Bradford assay (Protein Assay Dye Reagent Concentrate 5×, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was determined using Protein Assay Dye Reagent Concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
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bioRxiv - Pathology 2022Quote: ... 2-5%β-mercapto-ethanol for 10min at 70°C and then migrated with protein ladder (Precision plus protein all blue standards, Bio-Rad) in Bio-Rad gels (Criterion TGX ...