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Citations for Bio-Rad :
2301 - 2350 of 9412 citations for Recombinant Human PDCD1LG2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 10 uL of the protein loading mixture was loaded into the wells of either 12% mini-PROTEAN TGX precast protein gels (Bio-rad) or 12.5% SuperSep Phos-tag precast gels (Wako Pure Chemical Industries) ...
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bioRxiv - Biophysics 2019Quote: ... Gradients were manually fractionated into 200 μL fractions and protein concentrations were determined by the Bradford assay (Protein Assay Dye Reagent Concentrate 5×, Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a new tube and protein concentration was determined using Protein Assay Dye Reagent Concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
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bioRxiv - Pathology 2022Quote: ... 2-5%β-mercapto-ethanol for 10min at 70°C and then migrated with protein ladder (Precision plus protein all blue standards, Bio-Rad) in Bio-Rad gels (Criterion TGX ...
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bioRxiv - Immunology 2019Quote: ... Samples were then centrifuged for 30 min and protein concentration was measured using the DC Protein Assay according to standardized protocols (Bio-Rad). Protein concentration in supernatants was normalized to 1 mg/mL and frozen at −80°C until assay ...
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bioRxiv - Synthetic Biology 2020Quote: ... The boiled samples were centrifuged at 14,000 rpm for 10 minutes and 20 μg of total protein per sample was then loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA) and ran at 140 V ...
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bioRxiv - Immunology 2019Quote: Total protein concentration in bronchoalveolar lavage fluid (BALF) were measured by the Bradford Assay using Protein Assay Dye Reagent Concentrate (Bio-Rad) according to the manufacturer’s protocol ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted 1:20 in 1X PBS and protein concentration was quantified by the DC protein assay with BSA as the standard (BioRad®). Lysates were normalized to 5 μg/μL in 2X loading buffer (Lysis buffer supplemented with 5 mM beta-mercaptoethanol and Coomassie blue) ...
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bioRxiv - Molecular Biology 2019Quote: ... The results were normalized to total protein content in the corresponding cell RIPA lysates (Quick Start Bradford Protein Assay, Bio-Rad). The ELISA detects both the 163S and 157S chemerin.
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bioRxiv - Molecular Biology 2020Quote: ... Glycerol was added to the supernatants (final concentration: 10%) and protein concentrations were adjusted using Bio-Rad protein assay (Bio-Rad). The protein samples were diluted in 6x loading buffer (9.8% glycerol ...
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bioRxiv - Molecular Biology 2021Quote: ... The amount of protein in the extracts was assessed by the Bradford method using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad). Protein electrophoresis in 10% or 12% PAA gels with SDS in 1x Laemmli buffer was carried out ...
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bioRxiv - Molecular Biology 2021Quote: Self-association of GST-SP proteins was determined by native gel electrophoresis using Mini-PROTEAN TGX 4–20% precast polyacrylamide protein gels (Bio-Rad). Purified wild-type or mutant GST-SP (5 μg ...
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bioRxiv - Microbiology 2019Quote: ... we prepared a mixture of 25 µL each of Protein A and Protein G SureBeads Magnetic Beads (Bio-Rad, Hercules, CA) and immobilized 1–2 µg of primary antibody on the beads by rotation at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Precision Plus Protein All Blue and Kaleidoscope Pre-stained Protein Standards molecular weight ladders were purchased from Bio-Rad (Hercules, CA). Vectashield mounting media with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was used to quantify the total protein concentration in the sample using the DC protein assay (Bio-Rad) a BSA standard curve [34] ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic biotinylation with BirA ligase was performed and protein was purified by size-exclusion fast protein liquid chromatography using a NGC Quest 10 Chromatography System (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... Protein sample loading buffer was added to cell lysates and proteins were separated on SDS-PAGE (BioRad Criterion TGX Precast Gel). Antibodies used for immunoblotting are listed in Supp ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Bioengineering 2020Quote: ... The concentration of each fraction of proteins was determined by SDS-PAGE and quantitatively by Bradford protein assay (BIO-RAD, USA).
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bioRxiv - Genetics 2021Quote: ... Protein concentration in each sample was measured through Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, USA) and spectrophotometry at 595 nm (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: ... 10-50 μg of protein was loaded onto 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and transferred to polyvinylidene difluoride (PVDF ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Molecular Biology 2021Quote: ... equal amounts of protein for each sample was loaded on 4-15% Criterion TGX pre-cast midi protein gel (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Physiology 2021Quote: ... 5 ul of each supernatant sample was used to quantify the total amount of protein with a BCA Protein Assay Kit whereas the rest was diluted in 4x Laemmli sample buffer (Bio-Rad) with 2-Mercaptoethanol (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: Bradford assays were performed to quantify the concentration of total soluble protein (TSP) by using a protein assay dye reagent (Bio-Rad). For each BSA standard ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was estimated colorimetrically using a Bio-Rad protein assay and 25 μg of protein with equal volume of 2X Laemmli sample buffer (Bio-Rad) was denatured at 70°C for 10 min ...
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bioRxiv - Immunology 2021Quote: Lysates were centrifuged for 15 minutes at 4°C to recover supernatant and quantified for protein concentration using Protein Assay Dye Reagent Concentrate (Bio-Rad). 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Purified products were analyzed by 10% TBE PAGE and total protein quantified with Bio-Rad protein quantification reagent (Bio-Rad, 5000006). LPS contamination after conjugation was below 0.5 EU/mg as mentioned below in the vaccinations section.
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Microbiology 2022Quote: ... Equivalent protein amounts (EVs and proteins) were separated on 4-15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was assayed by Bradford’s method and 60ug of protein/sample was electrophoresed (Bio-Rad, USA) on a 5%/10% (loading/separating ...
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bioRxiv - Biochemistry 2022Quote: ... The protein concentration was measured on Tecan (Schoeller) and approximately 20 μg of protein lysate was loaded on the 10-well protein gels (Mini-PROTEAN TGX, Bio-Rad) and run at 120 V ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on 10% or 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Pierce™ Protease& Phosphatase Inhibitor Mini Tablets) and final protein concentration was determined with BCA protein Assay (Biorad Laboratories, Feldkirchen, Germany). Heart tissue samples of the infarct areas were excised (2 weeks or 8 weeks post-surgery ...
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bioRxiv - Biophysics 2024Quote: ... total proteins and biotinylated proteins were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Microbiology 2024Quote: ... The same amounts of proteins were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat:4561096) or homemade 6-15% SDS-PAGE gels and transferred to a nitrocellulose membrane (Biorad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Protein purity was analyzed by SDS-PAGE and the protein concentration was determined using the Bradford assay (QuickStartTM, Bio-Rad, Germany) with BSA as the protein standard (Zor & Selinger ...
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bioRxiv - Microbiology 2024Quote: ... These concentrations were used to load equal sample of proteins into Any-kDTM Mini-PROTEAN® TGXTM Precast Protein Gels (BioRad) for SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...