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Citations for Bio-Rad :
2301 - 2350 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were run on 4-20% precast polyacrylamide gel (4-20% Mini-PROTEAN TGX Precast Protein Gels, BioRad) to separate them and transferred onto PVDF membranes using semi-dry transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were passed through a 26G syringe and protein was quantified by DC Protein Assay (Bio-Rad, cat #5000112). Lysates were diluted to equal volumes and 30 ug protein was loaded per well using the Novex WedgeWell Tris-Glycine Welcome Pack (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each lysate was analyzed using Bio-Rad protein assay reagent according to the manufacturer’s instructions (Biorad). Luciferase units obtained were normalized to the protein content of each well ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µg total protein was loaded on AnykD™ Criterion™ TGX™ Precast Midi Protein Gel (Biorad, #5671125) and 60 kDa region was excised from the Coomassie stained gel ...
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bioRxiv - Biophysics 2024Quote: ... Total protein concentration in purified microsomes and RyR1 fractions was measured with Quick Start Bradford Protein Assay (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration was determined by Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 20µg of protein was loaded into a 4-15% gradient mini-PROTEAN TGX precast protein gel (4561086, Bio-Rad). Gels were run at 150V in 1x Tris/Glycine/SDS buffer (1610772 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the samples was determined by the BioRad Protein Assay method (BioRad Laboratories, Hercules, CA, USA). For our gels ...
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bioRxiv - Molecular Biology 2024Quote: ... 10µg of total protein was loaded per lane onto a 4-20% Criterion Tris-HCl Protein gel (Bio-Rad) and separated by electrophoresis at 150 V for 1hr ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 10 min and the protein concentration was determined using a Lowry assay (DC Protein Assay, BioRAD). Nucleic acid coated beads were incubated with 2 mg protein of cell lysates for 60 min and then washed three times with TAP buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Elution of biotinylated protein was performed by heating the beads in 30LμL of 2X protein loading buffer (1610737; BioRad) supplemented with 2LmM biotin + 20LmM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations were determined by Bradford Assay with the Bio-Rad Protein Assay Dye reagent concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... protein was separated by SDS-PAGE on a 4-15% Criterion TGX pre-cast midi protein gel (Bio-Rad), and transferred to nitrocellulose membrane using a standard tank electrotransfer protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were separated by SDS-PAGE and fluorescent proteins were visualized using the ChemiDocTM MP Imaging system (Bio-Rad). The protein gel was then stained in Coomassie blue solution.
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT) until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50mM Tris-HCl (PH=8),150mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Identical amounts of proteins (20-40μg) were electrophoresed on 4–15% Mini-PROTEAN TGX precast protein gels (Bio-Rad). For transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein concentrations of sample supernatants were measured with the Bio-Rad protein assay dye reagent (Bio-Rad, 5000006). Equivalent amounts of protein samples were mixed with Laemmli buffer (100 mM Tris-HCl pH 6.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Identical amounts of proteins (20-40μg) were electrophoresed on 4–15% Mini-PROTEAN TGX precast protein gels (Bio-Rad). For transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The total protein concentration was determined using Bradford protein assay according to the instructions from the manufacturer (Bio-Rad). POSTN protein expression from the ELISA assay was normalized with the total protein levels ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (30–50 μg) were loaded onto 4%–20% Mini-PROTEAN TGX Precast Protein Gels (BioRad). Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad). Proteins were transferred to the PVDF membrane using a Trans-blotter (Biorad) ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration das measured of each eluate sample in a 1:20 dilution by Bradford (BioRad protein assay). Per sample ...
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bioRxiv - Cell Biology 2024Quote: ... 1 tablet EDTA-free protease inhibitors) and protein content was determined using a Bradford protein assay (Bio-Rad, #5000001). 50-100 µg protein was run on an SDS-PAGE and blotted using a wet-transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: The total amount of protein present in the samples was quantified using the Bio-Rad Protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: Protein samples from mouse tissue were prepared by diluting 20-40 μg of protein with 1X Laemmli buffer (Biorad) supplemented with beta-mercaptoethanol (Sigma ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The protein concentration in the thylakoid membrane fraction was determined via Bradford’s method (Protein Assay, Bio-Rad, Hercules, CA) using a bovine serum albumin solution of known concentration as a standard.
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bioRxiv - Immunology 2024Quote: ... Proteins were separated by electrophoresis using 4-12% Bis-Tris mini protein gels and transferred to PVDF membranes (BioRad). Blots were incubated with primary antibodies at 4°C and then with HRP conjugated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... following gel electrophoresis proteins were transferred from the gel to a polyvinylidene fluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad, Trans-Blot turbo RTA transfer kit) using the Bio-Rad standard protocol (25 V,30 min) ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Biochemistry 2020Quote: ... Detection antibodies used: anti-human F(ab’)2 IgG-HRP (STAR126P, Bio-Rad), anti-His5-Tag IgG-HRP (MCA5995P ...
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bioRxiv - Microbiology 2022Quote: ... and Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, California, USA) were used to detect urinary cytokines ...
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bioRxiv - Microbiology 2022Quote: ... and a goat anti-human IgG conjugated to HRP (Bio-Rad, cat. 204005) was used as secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were analyzed using a human Th17 cell cytokine panel multiplex (BioRad 171AA001M). For flow cytometry ...
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bioRxiv - Bioengineering 2020Quote: ... or AlexaFluor647-conjugated mouse-anti-human CD120a (TNFR1) antibodies (clone H398, Bio-Rad). DAPI was used for dead cell exclusion.
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bioRxiv - Immunology 2023Quote: ... and Bio-Plex Pro Human IgA detection antibody (Bio-Rad, Cat No. 12014669). For both kits ...
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bioRxiv - Pathology 2023Quote: ... Immunoglobulins were analysed using a bio-plex pro human isotyping assay (Bio-Rad) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and Bio-Plex Pro Human Cytokine Screening Panel Standards (BIO-RAD, Hercules, CA). Cytokine and chemokine concentrations refer to 8-point standard curves.
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were fragmented for 10 minutes on ice in 0.2 N NaOH and purified on Bio-Spin P30 columns (Bio-Rad). Biotin-labelled RNA was then purified using MyOne Streptavidin C1 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was transferred to a Hybond-N+ Nylon transfer membrane (Cytiva) with a Trans-Blot SD blotting apparatus (BioRad) followed by crosslinking at 0.12 J/cm2 in a CX-2000 UV crosslinker (Analytik Jena) ...
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bioRxiv - Biochemistry 2023Quote: ... 2mM DTT and 0.02% n-dodecyl-β-D-maltopyranoside [DDM]) buffer after transferring into Econo columns (Biorad, Cat. no. 7372512). Thrombin at concentration 1unit µL-1 was added to the resin slurry at 1:1 (dry resin:cleavage buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Heads (N = 15 for each genotype) were decapitated and homogenized in 100 µl 2X Laemmli Sample Buffer (BIO-RAD 1610737) containing 5% of 2-Mercaptoethanol (Sigma-Aldrich M3148) ...
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bioRxiv - Immunology 2023Quote: ... Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad, Cat Nos. 12015406 and 12014773), and Bio-Plex Pro Human IgA detection antibody (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... dimethyl sulfoxide (DMSO) was added and the absorbance of each well was measured at 490 nm using an ELISA microplate reader (Bio-Rad, Hercules, CA, USA) (33).
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then sonicated and their protein content was quantified using the Bradford protein assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Each gel was loaded with a Precision Plus Protein™ Dual Color Standards as a protein marker (Bio-Rad, 1610374). Gel electrophoresis was stopped when the Bromophenol blue dye reached the bottom of the gel ...