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Citations for Bio-Rad :
2301 - 2350 of 2598 citations for Anti CD123 Antibody FITC labled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... HRP conjugated antibodies were detected using SuperSignal West Dura Chemiluminescent Substrate (Thermo Scientific and ChemiDoc XRS and Image Lab 5.1 (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Signal detection was done with HyGLO™ quick spray chemiluminescent HRP antibody detection reagent (Denville Scientific) and Chemidoc Touch Imaging System (BioRad) and quantified by ImageJ software.
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bioRxiv - Neuroscience 2021Quote: ... analyzed by immunoblotting with the indicated antibodies and visualized using enhanced chemiluminescence (Pierce) on a Bio-Rad Versadoc 4000 (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and later analyzed using a ChemiDoc™ Imaging System (Bio-Rad). Data is presented as ratio of phosphorylated/total protein normalized internally to each uninfected condition.
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bioRxiv - Cancer Biology 2021Quote: ... Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using the Chemi-Doc XRS+ system (Bio-Rad). Primary antibodies used were anti-BCL-XL (abcam ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Genetics 2022Quote: ... Blots with HRP-linked secondary antibodies were additionally incubated for 1-5 minutes with Clarity or Clarity Max ECL Western Blotting substrates (Bio-Rad). Blots were then imaged on a ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v5.2.1).
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bioRxiv - Cell Biology 2019Quote: ... Protein bands were visualized via enhanced chemiluminescence (Pierce ECL Western blotting substrate) using horseradish peroxidase-conjugated secondary antibodies and imaged using the ChemiDoc system (Bio-Rad). The quantifcation of the band was performed with ImageLab software (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was incubated with secondary HRP-conjugated antibodies and signals were detected using a chemiluminescent method (ChemiDoc XRS, Bio-Rad).
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bioRxiv - Immunology 2019Quote: ... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated with secondary antibodies for one hour at room temperature and developed using Clarity ECL Detection Kit (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were chosen according to the species of origin of the primary antibodies and detected by an enhanced chemiluminescence system (Bio-Rad). Densitometric analysis of the relative expression of the protein of interest versus the corresponding control (β-actin or total STAT3 ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were probed with appropriate secondary antibodies conjugated with HRP and imaged with a ChemiDoc XRS digital imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Primary and secondary antibodies were used as per the manufacturers’ instructions and membranes were imaged on the ChemiDoc MP (Bio-Rad) after incubation in Clarity Western ECL Substrate (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... 7 μm cryosections were methanol fixed for 20 minutes on ice and stained with an antibody specific to CD68 (clone: ED1, Bio-Rad). Animal protocols were approved by the University of Virginia Institutional Animal Care and Use Committee.
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bioRxiv - Biophysics 2019Quote: ... was used and the signal from HRP-conjugated secondary antibodies was detected with an ECL system and an enhanced chemiluminescence system (ChemiDoc Touch System, Bio-Rad). Quantification of the mean band intensity relative to the loading control Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with a secondary antibody and 1 × TBST washes the membrane was coated with chemiluminescent substrate (Clarity Max ECL substrate, Bio-Rad) and imaged on Chemidoc MP (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated for mouse (CST 7076) or rabbit (CST 7074) were used with Clarity-ECL chemiluminescence reagent (Bio-Rad) for detection ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen-primary antibodies complexes were detected with secondary antibodies conjugated to horse radish peroxidase and developed using a chemiluminescent reagent (Clarity ECL, Bio-Rad). Images of the WBs were acquired using a ChemiDoc MP System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... incubated with specific primary antibodies for 1 h and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies by using ClarityTM Western ECL Blotting Substrate (Bio-Rad). For pulldown assays between cell extract and purified Nyx and MAG mutants ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... The blots were probed for 1 hour at room temperature with α-CaHsp9031 diluted 1:10,000 in PBST with 0.1% milk and washed with 1x PBST prior to probing with α-rabbit antibody-HRP conjugate (BioRad #1705046) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The primary antibody was removed the next day and the membrane was incubated with secondary antibody at room temperature for 1 h. Enhanced chemiluminescence substrate (Clarity Western ECL Substrate, Catalog no. 1705060) from Bio-Rad Laboratories (Mississauga ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity-ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Molecular Biology 2020Quote: Western blot detection was performed by enhanced chemiluminescence using HRP-coupled secondary antibodies and the Clarity or Clarity Max substrates (Bio-Rad). The signal was digitized using Amersham 600RGB imager as 16-bit grayscale TIFF files ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).
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bioRxiv - Bioengineering 2022Quote: ... Antibody in the hybridoma supernatant was purified by passing through a column of DEAE Affigel blue (Bio-Rad, Sydney, NSW, Australia), dialysed into a Tris-saline buffer (Tris 10 mM ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies were applied at room temperature for one hour and the blot was visualized using a ChemiDoc MP System (Bio-Rad). RBM20 bands were quantified with Image Lab software and normalized to the γ-tubulin loading control.
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies and detection using Clarity Western enhanced chemiluminescence substrate (Bio-Rad Laboratories).
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with appropriate secondary antibodies before developing with Perkin-Elmer ECL Western Lightning Plus solution and visualized using a Chemidoc imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... then incubated at RT for 1 h with HRP-linked secondary antibodies (Bio-Rad 170-6515 and 172-1011, 1:5000), imaged via chemiluminescence on a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was then incubated with an HRP-conjugated secondary antibody for two hours at room temperature and developed with ClarityTM Westrern ECL substrate (Bio-Rad). Images were acquired using the ChemiDocTM imaging system (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... the membrane was washed with 1X PBS and thereafter incubated with HRP-conjugated secondary antibody for 1 hour at room temperature and detected using ECL reagent (BioRad, USA) under chemiluminescence ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with an HPR-conjugated secondary antibody and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with an HPR-conjugated secondary antibody and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad). Signal intensities were measured in ImageLab Software ...
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bioRxiv - Molecular Biology 2023Quote: ... blots were incubated with secondary antibodies for 1 h and were consequently developed using Clarity Western ECL and Clarity Max Western ECL substrates (Bio-Rad). Protein bands were visualized on the ChemidocTM MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary HRP-linked antibody was incubated for 1 hour at room temperature and developed using Clarity Western ECL blotting substrate (BioRad, 1705061).
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were subsequently probed with a primary antibody against β-ACTIN as a loading control or stained with Bio-Safe Coomassie stain (Biorad), dried ...
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP and the Clarity Western enhanced-chemiluminescence (ECL) substrate as describes by the manufacturer (Bio-Rad). Images were acquired and analyzed with the ImageQuant Las 4000 luminescent image analyzer.
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...