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Citations for Bio-Rad :
2201 - 2250 of 2612 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The amplified products were analyzed on a 2% agarose gel stained with SYBR safe and imaged using the Gel-documentation system (Bio-Rad, Hercules, CA).
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... Ten μg (final volume 2 μl for all samples) of serum proteins were loaded onto a nitrocellulose membrane (Bio-rad Laboratories, CA, USA). Then ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of Dicer-Like 2 (DCL2) (Glyma.09g025300) and Argonaute 1 (AGO1) (Glyma.09g167100) was assessed using iTaq Universal SYBR Green Supermix (BioRad, Hercules, CA, USA). To quantify F ...
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNA was amplified by qPCR with specific primers (Supplementary Table 2) using the C1000™ thermal cycler with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories). The relative expression of genes was analyzed quantitatively by the ΔΔCt method ...
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bioRxiv - Microbiology 2024Quote: ... Target bands were visualized by 2% agarose gel electrophoresis with GelRed staining and recorded by a gel imager (GelDoc; Bio-Rad, California, U.S.).
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bioRxiv - Biophysics 2024Quote: ... and washed with TBST (2 × 1 min) and TBS (1 × 1 min) before imaging using Clarity western enhanced chemiluminescence substrate (Bio-Rad, cat. # 1705061). Membranes were always stained first with H3 (C-term ...
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Biochemistry 2021Quote: ... A goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) antibody with HRP conjugate (1706515 and 1706516, respectively, Bio-Rad, Hercules, CA) was used as a secondary detection antibody ...
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bioRxiv - Plant Biology 2021Quote: ... the gel was rinsed with acetic acid/methanol (Destain, Bio-Rad). Each lane was cut into 4 bands ...
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bioRxiv - Microbiology 2020Quote: ... 10% acetic acid v/v and brilliant Coomassie blue (BioRad G250). For Western blotting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10% glacial acetic acid and imaged on a GelDoc (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the bicinchoninic acid assay (Bio-Rad), and the purified proteins were stored at −80°C until used.
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bioRxiv - Cancer Biology 2023Quote: ... Ten micrograms of total protein extract underwent electrophoresis in stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, Hercules, CA) and transferred onto PVDF membranes (Hybond-C Extra ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-rabbit IgG (H + L)-HRP (1:5000, BioRad,1706516) and anti-mouse IgG (H + L)-HRP (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... goat anti-rabbit IgG (H+L)-HRP conjugate (both Biorad) or Veriblot IP detection reagent (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... goat anti-mouse IgG (H+L)-HRP conjugate (Biorad 1706516), AffiniPure goat anti-rabbit IgG (H+L)-HRP conjugate (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... and Goat Anti-Mouse IgG (H+L)∼HRP Conjugate (BioRad). Membranes were developed with SuperSignal® West Pico Luminol/Enhancer Solution (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse IgG (H/L):HRP (AAC10P, Bio-Rad), anti-FLAG-Tag (M2 ...
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bioRxiv - Molecular Biology 2024Quote: ... goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad; 1706515); and goat anti-mouse IgG (H+L)-HRP conjugate (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) was applied in 1:10,000 dilution.
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase-conjugated Goat anti-Mouse IgG (H+L) (BioRad) (1:2000 ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was performed using 3.5 μl of diluted cDNA and 5.5 μM primers listed in Table 2 (C.-M. Lee and Thomashow, 2012; Wu et al., 2008)using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, cat# 1725121) with the CFX 384 TouchTM Real-Time PCR Detection System (Bio-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... This mix was transferred to a pre-chilled 2 mm electroporation cuvette and electroporation was performed (2.0 kV, 25 μF, 200 Ω) using a Gene Pulser Xcell (Bio-Rad Laboratories, Hercules, CA) electroporation sys-tem ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated together at RT for 5 min in the presence and absence of 5 mM 2-OG prior to loading on the analytical ENrich 650 column (BioRad Laboratories, Inc., Hecules, USA). The column was equilibrated with 50 mM Tris/HCl and 150 mM NaCl and supplemented with 5 mM 2-oxoglutarate when the complexes were preincubated in the presence of 5 mM 2-oxoglutarate and the applied proteins isocratic eluted with the respective buffer with a flow rate of 1 ml min-1 ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Genetics 2022Quote: ... of each mating type of the strains of interest were placed in 2 mL tubes containing 500 μL H2O and ground using a FastPrep (TeSeE - Biorad, Hercules, California, United States) (20s ...
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bioRxiv - Synthetic Biology 2020Quote: The constructs were transformed into Agrobacterium tumefaciens strain pGV3850 by electroporation (2 mm cuvette, 2.5 kV, 400 Ω, and 25 μF) in a Bio-Rad GenePulser (Bio-Rad, Hercules, CA, USA). Cells were incubated at 28°C for 2 h after 200 μl LB media was added ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Neuroscience 2022Quote: ... Solid phase specificity was confirmed with dot blots of serial antigen dilutions (2 μL) applied to nitrocellulose membrane (Bio-Rad 0.45 µm; Fig. 1b). Membranes were allowed to air dry (1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were performed in total 24ul volume using 12ul of 2 x ddPCR Supermix for Probes (No dUTP) (Bio-Rad Laboratories, catalog no. 1863024), 900nM of target primer pairs ...
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bioRxiv - Biochemistry 2022Quote: ... We mixed 10 μL of each fraction with 2 μL of denaturing RNA loading dye and loaded it into a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Urea-PAGE (7.5% ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) and 400 nM of forward and reverse primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of total RNA was reverse-transcribed to single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad Hercules, CA, USA). RT-qPCR was run on Lightcycler 480 (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... under reducing conditions using 2-ME and transferred onto polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo system (Bio-Rad, Hercules, CA, USA). After blocking with 10% skim milk (Becton Dickinson ...
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bioRxiv - Genetics 2024Quote: ... 2-fold serial dilutions of tick DNA from 8ng/μl to 0.125ng/μl using IQ™ SYBR® Green Supermix (Bio-Rad, C#64313832) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... the plate was held at 4 °C until the lid temperature cooled to 37 ºC and could be stored at 2 °C to 8 °C overnight before analysis on the QX600 Droplet Reader (Bio-Rad Laboratories, California, USA).
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bioRxiv - Molecular Biology 2020Quote: ... and the gel was stained with Coomassie Brilliant Blue R-250 (Bio-Rad) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Gel was de-stained with Coomassie blue R-250 destaining solution (Bio-Rad) for 2 hours with 3 times buffer changes ...
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bioRxiv - Immunology 2023Quote: ... Transmembrane was performed using a Trans-Blot○R Turbo Transfer System (Bio-rad). The membranes were blocked for 1hr at room temperature with 5% non-fat milk (Research Products International ...
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bioRxiv - Pathology 2023Quote: ... Gels were then stained with Coomassie Brilliant Blue R-25 solution from BioRad for 30 min ...