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Citations for Bio-Rad :
2051 - 2100 of 2446 citations for Anti CD11b Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... were used as secondary antibody and detected using the Clarity Western ECL Substrate as described by the manufacturer (Bio-Rad). Images were acquired and analyzed with the ImageQuant LAS 4000 luminescent image analyzer.
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bioRxiv - Biochemistry 2021Quote: ... The same samples were also detected by HRP conjugated StrepTactin antibody at 1:10000 dilution (Bio-Rad catalog number 161038). To allow detection of endogenous selenok ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
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bioRxiv - Immunology 2022Quote: ... Primary antibodies (for further specification see key resource table, table 1) were diluted in blocking solution (F4/80 [Bio-Rad] ...
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bioRxiv - Microbiology 2019Quote: ... Blots were probed for FlgR using FlgR antisera raised in rabbit and developed using HRP-conjugated goat-antirabbit secondary antibody with a ChemidocMP imager (BioRad)
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bioRxiv - Pathology 2020Quote: ... Antibody binding was detected with electrochemiluminescence substrate (#12757P; CST) and chemiluminescence visualized with ChemiDoc™MP Gel Imaging System (BioRad).
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bioRxiv - Physiology 2021Quote: ... Membranes were incubated with HRP-conjugated secondary antibodies and visualized by enhanced chemiluminescence on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose strips were blocked in PBS with casein for 30 mins at room temperature and then probed for 1 hour with monoclonal antibodies (mAb) recognizing H3 HA (clone 36/2 (26)) or IAV M1 matrix protein (clone MCA401, BioRad). Strips were washed and incubated for 1 hour with a horse radish peroxidase (HRP)-conjugated secondary immunoglobulin (DAKO) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with secondary antibody for 1 h and proteins were revealed by ECL Prime (Amersham) using ChemiDoc Touch imager (Biorad).
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Pathology 2019Quote: The target protein bands were detected with a horseradish peroxidase (HRP)–conjugated antibody (170-6515, 172-1011, 1720-1011, 1:1,000; Bio-Rad), which was visualized by chemiluminescence with Clarity Western ECL substrate (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... Signal was detected with corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies and Clarity Western ECL substrate (170-5061, Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... The blots were then washed 3x with TBS-T for 10 min each and incubated with the horseradish peroxidase (HRP) conjugated specific secondary antibodies (1:20,000, Bio-Rad) for 1 h at RT ...
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bioRxiv - Physiology 2021Quote: ... membranes were incubated with peroxidase-conjugated secondary antibodies and visualized by enhanced chemiluminescence using Clarity Western ECL substrate (Bio-Rad). Target protein levels were normalized against total protein (as determined by Amido Black staining).
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bioRxiv - Neuroscience 2021Quote: ... the membranes were probed with appropriate secondary antibodies conjugated with Alexa488 fluorophore and imaged using the ChemiDoc system (Bio-Rad). The dot intensities were quantified with the Image Studio Lite software (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were scanned the following day after 1 hour incubation with secondary antibodies using Odyssey CLx Imaging System or ImageQuant system (BioRad, HRP anti-rabbit or anti-mouse (CST ...
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bioRxiv - Microbiology 2021Quote: hCMP-mRBD protein kinetic binding studies to ACE2-hFc and CR3022 antibody were performed on a ProteOn XPR36 Protein Interaction Array V.3.1 (Bio-Rad). The GLM sensor chip was activated with sulfo-NHS and EDC (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Corresponding mouse or rabbit horseradish peroxidase-conjugated secondary antibodies (diluted 1:10,000 in 1x TBST) were added for 1 hr at room temperature (Bio-Rad). Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then transferred to fresh low- protein binding tubes and antibody-protein complexes were eluted by incubating in 60 μL 1x Laemmli Sample Buffer (Biorad, cat ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature prior to development with clarity ECL (BioRad) developing reagents with a ChemiDoc Touch (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... After 3x 10 min washes in TBST appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature and developed with a ChemiDoc Touch (BioRad) as described above.
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bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated with HRP or fluorophore-conjugated secondary antibodies and revealed by chemiluminescence (Clarity Max Bio-Rad, 1705062) or fluorescence (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... the sample was separated in SDS-PAGE after addition of Laemmli SDS loading buffer and transferred onto the PVDF membrane followed by probing with specific antibodies for visualization under Chemidoc (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated 1 h with horseradish peroxidase-conjugated secondary antibodies (1:5,000) and the bands were visualized by chemiluminescence using the Clarity Western ECL substrate (Bio-Rad) and a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... HA-tagged proteins were detected using primary antibody anti-HA (1/10 000) followed by incubation with the HRP-conjugated secondary antibody and finally revealed by chemiluminescence (Clarity Western ECL, Biorad) on a Fusion-FX imager (Vilber).
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bioRxiv - Cell Biology 2023Quote: ... The signals from antibodies were detected using enhanced chemiluminescence via a Bio-Rad ChemiDoc MP System (Bio-Rad, Hercules, CA). Following detection of HA ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... After three washing steps membranes were incubated with respective peroxidase-conjugated secondary antibodies and signals were detected using chemiluminescence with a ChemiDoc MP Imaging System (Biorad). Antibodies and dilutions are listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Cell Biology 2023Quote: ... Signals from HRP-conjugated secondary antibodies were detected by enhanced chemiluminescence (SuperSignal® Pico or Femto, ThermoScientific) using ChemiDoc (Biorad). Antibodies used in this study are listed in Table S6.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... Blots were rinsed and probed in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000; BioRad, Hercules, CA, USA) for 30 min at RT in 2.5% NFDM or BSA in 1× TBST ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Cell Biology 2023Quote: ... And then blots were probed with secondary antibodies for 1h at room temperature before ECL development and imaging (Bio-Rad). The primary antibodies used for immunoblotting are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The appropriate HRP-conjugated secondary antibodies were used for the chemiluminescent detection of proteins with Clarity Max Western ECL Substrate (BioRad). Membranes were scanned with a ChemiDoc imaging system (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with horseradish-peroxidase-linked secondary antibodies diluted 1:5000 followed by exposure to Clarity Western ECL Substrate (Cat. No. 170-5060, BioRad) and film development ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed in 1x TBST with mild agitation before incubation with horseradish peroxidase-conjugated secondary antibodies diluted in blocking buffer (α- mouse 1:10,000 (170-6516; BioRad), α-rabbit 1:10,000 (170-6515 ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were rinsed and probed in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000; BioRad, Hercules, CA, USA) for 30 min at RT in 2.5% NFDM or BSA in 1× TBST ...
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bioRxiv - Microbiology 2023Quote: ... blots were washed twice in TBS with 0.05% Tween 20 and then incubated with a goat α-rabbit secondary antibody conjugated to horseradish peroxidase (Biorad) at a 1:5 000 dilution in TBS with 0.05% Tween 20 and 5% milk for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody cocktail against CD68 (rat monoclonal; 1:250; catalog no. MCA1957; Bio-rad), GFAP (Chicken polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary horseradish peroxidase linked antibody was incubated for 1 hour with gentle agitation at room temperature and developed using chemiluminescence blotting substrate (1705061, BioRad or 34095 ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Biophysics 2023Quote: ... the membrane was treated with HRP-conjugated secondary antibody and later stained and imaged for chemiluminescence using freshly prepared Clarity ECL substrate (Biorad). All information regarding the reagents has been provided in Table S3.
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bioRxiv - Biochemistry 2023Quote: ... in a wet tank apparatus, probed with the indicated primary antibodies, and detected with ECL (ECL Select, Cytiva #RPN2235) in a ChemiDoc XR (BioRad). Images were analyzed with ImageLab software (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... We utilized colorimetric detection to visualize the apoAI-EfpA band(s) on the membrane after WB transfer: The primary mouse antihistidine tag antibody (BioRad) and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...