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Citations for Bio-Rad :
151 - 200 of 8961 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the isolates using the InstaGeneTM Matrix comprising 6% (w/v) Chelex resin for PCR-ready DNA purification (Bio-Rad #7326030; California, United States). DNA quality was checked by measuring the A260/280 ratio using NanoDrop ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using the SsoAdvanced universal SYBR green supermix kit (Bio-Rad, Bio-Rad CFX connect RT-PCR machine). Custom primers were used for gene amplification (Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... and relative transcript levels were measured for each sample in triplicate by quantitative qRT-PCR using SYBR Green PCR Kit (Biorad), on a MyiQ™ Real time PCR system (Biorad) ...
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bioRxiv - Microbiology 2020Quote: cDNA synthesis and quantitative reverse transcription PCR (qRT-PCR) were performed using the iTaq Universal SYBR Green One-Step Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using iScriptTM cDNA synthesis kit and qRT-PCR was performed using a CFX96 Touch Real-Time PCR Detection System (BioRad). Data were analyzed using the following equations ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using iScript Select cDNA Synthesis Kit (Bio-Rad). qPCR was performed on a CFX Connect system (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... using the iProof High-Fidelity PCR Kit (Bio-Rad Laboratories, Oslo, Norway). The following cycling protocol was used for all three genes ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-PCR was performed using iScript Select cDNA synthesis Kit (BIO-RAD) and high-fidelity Phusion DNA polymerase (New England Biolabs) ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Quantitative RT-PCR was run using the SsoFast EvaGreen Supermix kit (Biorad) on a Quantstudio 3 thermocycler (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcriptional PCR (iScript cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) and SYBR-Green-based qPCR (SYBR Green Supermix ...
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bioRxiv - Neuroscience 2019Quote: ... unconjugated dye was separated and removed from the solution using a purification column packed with BioGel P-30 fine size exclusion purification resin (BioRad), which is designed to separate free dye from proteins with a molecular weight of greater than 40,000 kDa (MW ovalbumin = 45,000 kDa) ...
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bioRxiv - Microbiology 2023Quote: ... Products were purified using the ssDNA clean and concentrate kit and loaded on 10% TBE-Urea gels (BioRad #4566033) with sample buffer (Thomas Scientific #C995V39 ...
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bioRxiv - Biochemistry 2021Quote: ... and a Profinia protein purification system (Bio-Rad) using wash (buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad). GAPDH was used as the invariant housekeeping gene internal control.
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bioRxiv - Molecular Biology 2019Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified using Qbit dsDNA HS Assay kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified with a dsDNA HS Assay kit on a Qubit fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... using the iScript one-step RT–PCR kit for probes (Bio-Rad Laboratories). Samples were run in 384-well formats in triplicates as multiplexed reactions with a normalizing internal control (36B4) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... using the iScript one-step RT–PCR kit for probes (Bio-Rad Laboratories). Samples were run in 384-well formats in triplicates as multiplexed reactions with a normalizing internal control (36B4) ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR (qPCR) was performed using iQ SYBR Green Supermix Kit (Bio-Rad) and a QuantStudio5 instrument (Applied Biosystems ...
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bioRxiv - Systems Biology 2023Quote: ... Quantitative real-time PCR was performed with the SsoFast EvaGreen Supermix Kit (BioRad) on a AriaMx Real-time PCR System (Agilent ...
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bioRxiv - Immunology 2019Quote: ... Quantification of gene expression was performed by using the KAPA SYBR-FAST One-Step qRT-PCR kit and the CFX-Connect Real-time PCR detection system (Bio-Rad Laboratories) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and ampR was measured by quantitative Reverse Transcriptase PCR (qRT-PCR) using the iTaq Universal SYBR Green One-Step kit (Biorad, Hercules, CA) and a CFX96 Touch Real-Time PCR detection system (Bio- Rad ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Genomics 2019Quote: Mammalian cells were embedded in gel plugs and High Molecular Weight DNA was purified as described in a commercial large DNA purification kit (BioRad #170-3592). Plugs were incubated with lysis buffer and proteinase K for four hours at 50°C ...
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed by using a HiScript II one-step qRT-PCR SYBR green kit (Vazyme) on a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Inc.). The following primers were used ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was analyzed by using quantitative real-time PCR according to the Biorad iScript One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers (F/R) ...
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bioRxiv - Systems Biology 2023Quote: ... Japan) and quantitative PCR using a KOD SYBR qPCR kit (TOYOBO) with CFX96 Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The digestion products were analyzed by 12-15% SDS-PAGE and stained with Coomassie blue or silver (Silver stain plus kit, Bio-rad). Images were taken using a Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced - Universal SYBR Green Supermix ...
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bioRxiv - Microbiology 2022Quote: Semi-quantitative PCR was performed using iProof High-Fidelity kit polymerase (BioRad, Temse, Belgium) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR was performed using the SYBR™ GREEN PCR Master Mix kit (Bio-Rad). Relative expressions of total RNA were normalized to endogenous control Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Microbiology 2021Quote: ... The iScript one-step RT-PCR Kit with SYBR Green (Bio-Rad, Hercules, CA) was used for RT-qPCR following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Iscript AdV cDNA kit for RT-PCR (cat# 1725038) was purchased from BIO-RAD. RNeasy Mini Kit (cat#74104 ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR reactions were performed using SsoAdvanced Universal Sybr Green Supermix kit (Bio-Rad), and analyzed using CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... T7-tagged templates were generated with the iProof High-Fidelity PCR Kit (Bio-Rad) using sequences cloned into plasmids pIB (LacZ) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reactions were assembled using the ddPCR Supermix for Probes (No dUTP) kit (BioRad). Each ddPCR reaction contained 1 µl cDNA and either Dbh and Tbp or Th and Tbp probes (250 nM each ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR was carried out using iScript RT Supermix (BioRad cDNA kit, cart#1708841) and iTaq Universal SYBY green Supermix kit (BioRad cat# 1725121 ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed using the iScript cDNA Reverse Transcription Supermix Kit (Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were imaged and quantitated (BioRad product info), using the H3 band as an independent load control to normalize the band intensities of H2B-Ub ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using a SYBR Green PCR Master Mix kit on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The reaction system (10 μL ...
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bioRxiv - Biophysics 2024Quote: ... Each purification step was analysed by SDS-PAGE (Bio-Rad) with Coomassie Blue stain to assess the protein purity ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced™ - Universal SYBR® Green Supermix ...
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bioRxiv - Genetics 2021Quote: ... followed by purification with RNeasy minicolumns (Qiagen, μg total RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, Des Plaines, IL). Assay of RNA via quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed by using Sso-Advanced Universal SYBR Green Supermix Kit (Bio-Rad) on CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Integration sites were amplified by nested PCR using the iProof High-Fidelity kit polymerase (BioRad). Two sets of primers were used in the PCR (sequences are listed in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... genomic DNA contamination and PCR reaction performance were included from the array kit (Bio-Rad). The reference genes ...