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Citations for Bio-Rad :
151 - 200 of 236 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2020Quote: 50 μL of PCR mastermix solution in Nuclease-free ultrapure water contained 60 mM Tris-HCl Buffer pH 8.8 (Bio-Rad), 200 μM dNTPs ...
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bioRxiv - Immunology 2021Quote: ... 200 µL of bead mix solution were added to all wells and washed using a Bio-Plex Pro Wash Station (BIORAD). 25 µL of Universal Assay Buffer were added to all wells ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 μL of PCR1 reaction (2 ng) were combined for the qPCR reaction using CFX 96 Touch Biorad qPCR thermocycler (Biorad). Δct was calculated by subtracting the mean Cq of each sgRNA to a non-targeting control ...
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed three times before the addition of HRP-conjugated anti-mouse IgG (H+L; Bio-Rad; 170-6516) at 1:3,000 and anti-mouse IgG2b (ab97250 ...
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bioRxiv - Bioengineering 2024Quote: ... Fully expanded leaves from in vitro cultured Nicotiana tabacum plants (L. cv. Petite Havana) were bombarded with 50 μg of 0.6-μm gold particles (Bio-Rad) coated with 10 μg of plasmid DNA using 1,100 psi rupture discs (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Molecular Biology 2024Quote: 5 μL of the final extraction elutions or the resuspended pellet were blotted onto a nitrocellulose membrane (0.2 μm; Bio-Rad). The transthyretin from the extractions was observed by using anti-transthyretin antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μl of these lysates (phospholipid concentration = 6.4 μM) was adsorbed to a nitrocellulose membrane using a Bio-Dot (Bio-Rad). After blocking with TBS-T containing 3% BSA for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µl of the ligation product was added to 50 µl of NEB 10-beta competent bacteria and transferred to an ice-cold 0.2 cm Gene Pulser cuvette (Bio-Rad) for electroporation (25 µF ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl of mixed primers containing 5 μM of each primer and 5 μl of iTaq Universal SYBR Green Supermix (BioRad), as previously described (Xavier et al. ...
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bioRxiv - Immunology 2024Quote: ... and dialyzed overnight at 4 °C into 4 L of Experimental Buffer (25 mM Tris, 100 mM NaCl, pH 7.4) with 2 g/L Chelex 100 resin (Bio-Rad) to chelate residual transition metal ions ...
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bioRxiv - Microbiology 2024Quote: ... 10.5 µL of total genomic DNA was combined with 12.5 µL of 2xEVA-Green master mix for unlabeled probes (Bio-Rad), 1 µL of HindIII-HF (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Fragmentation was stopped by addition of 80 μL 1 M Tris pH 6.8 and cleaned up twice with Micro Bio-Spin P-30 Gel Columns (BioRad, 7326223) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Immunology 2021Quote: ... 1/10 dilutions were used in triplicates with 0.2 μM primer and 10 μL LightCycler 480 SYBR Green I Master in a 20 μL reaction and qPCR executed in a 98- well block on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Absolute transcript levels for TDP-43 ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was subjected to affinity chromatography at 4°C composed of 75 μL of amylose resin bound to 200 pmol of MS2-MBP protein in a Bio- Spin disposable chromatography columns (Bio-Rad). After washing ...
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bioRxiv - Bioengineering 2022Quote: ... Approximately 20 μL of the denatured phage was loaded in a 4-20 % gradient gel (Mini-PROTEAN® TGX Stain-Free gel, Biorad) and separated by electrophoresis at 120 V ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed thrice before the addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (Bio-rad, 170-6516) at 1:3,000 ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... A chemiluminescent signal visualized by Chemi-Lumi One L (07880-70, Nacalai Tesque) was detected with a ChemiDoc Touch imaging system (Bio-Rad) and analyzed with Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... the dynamics of PEN reactions were recorded in 20 μL solutions inside 150 μL qPCR tubes using a Qiagen Rotor-Gene qPCR machine or a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). For biological relevance ...
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bioRxiv - Microbiology 2020Quote: ... Icecold freshly prepared competent cells (25 μl) were mixed with 4.5 μg of linear DNA and transferred to 0.1 cm-gap electroporation cuvettes (Bio-Rad, CA, USA). A pulse of 1.5 kV was applied to the cuvette with a MicroPulser electroporation apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... equipped with a diode array detector (L-7450) and separated on an AMINEX HPX-87H column (7.8 x 300 mm; BioRad, Munich, Germany) with a cation H guard pre-column ...
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bioRxiv - Neuroscience 2022Quote: ... at room temperature [goat anti-mouse 170-6516 or goat anti-rabbit 170-6515 IgG (H+L) HRP conjugate (Bio-Rad)] were all used at 1:2000 in 3 % BSA in 0.1 % Tween 20 (PBS/T) ...
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bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: LTA extracts (10 μl) were run on 15 % polyacrylamide gels and transferred to PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). After blocking with 5 % milk and 1 % BSA in TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... 20 µl of each reaction mix and 70 µl of oil were converted to droplets with the QX200 droplet generator (Bio-Rad). Droplet-partitioned samples were then transferred to a 96-well plate ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was then passed over 20 ml of poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was set up in a 20-μl volume with 100 - 200 ng of RNA and was conducted using the CFX Connect Real-Time PCR System (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... 1x DreamTaq buffer and 1 μL sample DNA in a total reaction volume of 10 μL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then passed over 20 ml of Poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000, 1721011, Bio-Rad, RRID: AB_11125936). Detection of the horseradish peroxidase signal was carried out using Clarity Western enhanced chemiluminescence (ECL ...
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bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then passed over 20 ml of Poly-L-proline conjugated beads in a disposable column (Bio-Rad, USA). The beads were first washed at room temperature in wash buffer (10 mM Tris pH 8 ...
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bioRxiv - Microbiology 2024Quote: ... and 1.5 µl H2O2 and applied to the membrane before the ECL signal was detected with a ChemiDoc XRS imaging system (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 60 μL of secondary goat antibody anti-rabbit IgG coupled with horseradish peroxidase (Bio-rad Goat Anti-Rabbit IgG (H+L) HRP conjugate 170-6515 ...