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Citations for Bio-Rad :
151 - 191 of 191 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Vector purity was assessed by 4–12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and fluorescent staining (Oriole, Bio-Rad, Hercules, CA, USA). A transfer plasmid (pAAV-CMV-eNpHR3.0-EYFP-WPRE ...
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bioRxiv - Cell Biology 2020Quote: ... The purity and integrity of the proteins was evaluated for each batch by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-15% gradient gels (Mini-PROTEAN® TGX® Precast Protein Gels, Bio-Rad) with a Precision Plus Protein® Kaleidoscope® (Bio-Rad ...
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bioRxiv - Pathology 2021Quote: ... Hippocampal samples with equal amounts of protein (40 μg) were separated by electrophoresis in 10% sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Bio-Rad Labs Inc.). Nonspecific binding sites on the membrane were blocked by incubation with 5% milk in Tris-buffered saline solution containing 0.1% Tween 20 at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... were mixed with 4× Bio-Rad sample buffer and loaded onto 4%–15% Trisglycine sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA). Proteins were transferred to supported nitrocellulose membrane and probed using Syn101 antibodies against aSyn (BD labs ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty micrograms of proteins for each well were separated on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel with pre-stained molecular weight markers (cat. num. 161-0374; Bio-Rad, Hercules, CA) and transferred to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed on 10% gels using the BioRad Mini-Protean Tetra Electrophoresis System (Bio-Rad, CA, USA). The gel was stained with Coomassie brilliant blue R-250 and was scanned on a gel imager (Bio-Rad Laboratories (Shanghai ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted proteins were analysed by 4—15% sodium dodecyl sulfate polyacrylamide (SDS-PAGE) gel electrophoresis (Mini-PROTEAN® TGX™ Precast Gels by Bio-rad) using broad range molecular weight markers (PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Neuroscience 2021Quote: ... total and surface labeled proteins were separated using a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (Bio-Rad, Feldkirchen, Germany). The membrane was blocked for 1 h in PBS-T supplemented with 5% skim milk and afterward incubated with 1:600 primary c-Myc-tag Polyclonal antibody (sc-789 ...
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bioRxiv - Biophysics 2020Quote: ... the samples were solubilized with standard Laemmli sample buffer and separated by electrophoresis using sodium dodecyl sulfate polyacrylamide gel prepared using Any kD TGX precast protein gels (Bio-Rad, Hercules, California). Separated proteins in gel were electroblotted onto a polyvinylidene difluoride membrane using Trans-Blot Turbo transfer system according to the manufacturer’s instruction (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... 20µg samples were boiled in gel loading buffer containing β-mercaptoethanol and sodium lauryl sulfate as a reducing agent and loaded onto AnyKD precast polyacrylamide gels (Bio-Rad 4569033) for protein separation ...
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bioRxiv - Microbiology 2023Quote: Proteins within tailocins were separated using a 4-20% sodium dodecyl sulfate-polyacrylamide gradient gel (Mini-PROTEAN TGX Stain-Free Precast, Bio-Rad Hercules, USA) electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... of cells lysates was subjected to electrophoresis on a sodium dodecyl sulfate (SDS) 7.5% or 4-20% polyacrylamide gel (4561024 or 4561095, respectively, Bio-Rad, California, United States). Proteins were semi-dry transferred to PVDF membranes (1704159SP5 ...
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bioRxiv - Bioengineering 2024Quote: ... were separated on a 4–10% gradient sodium dodecyl sulfate-polyacrylamide gel at 120 V for 90 min using a PowerPac Basic setup (Bio-Rad Laboratories, Inc.). Next ...
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bioRxiv - Immunology 2024Quote: Protein samples were prepared in Laemmli buffer and heated at 95°C for 5 min prior to resolution by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred onto nitrocellulose membranes (Biorad Trans blot turbo) ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... Equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to NC membranes (Bio-Rad, no. 1704158). 4× Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... in the HPT and HPC were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using TGX Stain-Free 12% gels (gels were visualized using ChemiDoc Imaging Systems, Bio-rad, Hercules, California, USA) and transferred to nitrocellulose membranes using Trans-Blot Turbo Transfer System (Bio-rad ...
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bioRxiv - Plant Biology 2020Quote: ... or self-made gel and subjected to a sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) using a Mini-PROTEAN® Electrophoresis cell (Biorad, Cat No 1658002EDU). Gels were run at 130 V for approx ...
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bioRxiv - Microbiology 2022Quote: ... Resulting lipopolysaccharides were separated using gradient 4–20% sodium dodecyl sulfate□polyacrylamide gel (4–20% Mini□PROTEAN® TGX™ Precast Protein Gel, BioRad, Hercules, USA) electrophoresis (SDS□PAGE ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of cell lysate or concentrated conditioned medium were resolved by sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membrane was blocked for 60min with 5% non-fat dry milk in Tris-buffered saline and Tween 20 (1:1000) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular proteins were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis before being electroblotted onto polyvinylidene fluoride membranes (#1704156, Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with the following primary antibodies for 60 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were resolved by sodium dodecyl sulfate-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA, USA). Blots were probed with the indicated primary antibodies listed in Extended Data Table 4 ...
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Equal protein amounts of the different samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose or polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories, Feldkirchen, Germany) by semidry blotting ...
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bioRxiv - Genetics 2023Quote: ... The purity of viral preparations was determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis on a 4– 15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Hercules, CA, USA) in Tris-glycine- SDS buffer (Figure S3).
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of each lysate sample were separated though sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Criterion precast gels (Bio-Rad Cat #456-1095). Proteins were blotted onto Polyvinylidene difluoride (PVDF ...
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The intersection of endocrine signaling and neuroimmune communication regulates neonatal nociceptionbioRxiv - Neuroscience 2024Quote: ... 20 µl of the mixtures were resolved along with Chameleon® Duo Pre-stained Protein Ladder (Lot #928-60001) in 10% precast Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE: Bio-Rad, cat# 456-1030). Samples were separated at 50 V for 30 minutes followed by 100 V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... equal protein concentrations were loaded onto and separated in 12% (wt/vol) sodium dodecyl sulfate polyacrylamide gel (40% acrylamide/bisacrylamide solution; Bio-Rad, Hercules, CA; 161 0148). Proteins were transferred from the gel to 0.45 m pore size μ nitrocellulose membrane (Maine Manufacturing ...
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bioRxiv - Immunology 2020Quote: ... Protein profile was analyzed using 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as described by Laemmli [11] and stained with Coomassie Brilliant Blue (Bio-Rad, Rockville Centre, NY 11571) [12] ...
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bioRxiv - Physiology 2024Quote: ... Equal amounts of protein samples (35 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Physiology 2024Quote: ... Equal amounts of protein samples (40 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electro-transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked with 5% non-fat milk dissolved in Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated in 10% or 12% sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad Life Science, Mississauga, ON). Blots were incubated overnight at 4°C in blocking buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Equal amounts of protein were subjected to 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (cat. no. 1620112; Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked using Intercept® (TBS ...
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bioRxiv - Plant Biology 2021Quote: ... Leaf disks were ground to a fine powder evenly with a grinding tool and 120 μl extraction buffer added (30 μl Laemmeli sample buffer 4x [Bio-Rad], 24 μl DTT 1 M, 66 μl dH2O). Tubes were vigorously vortexed for 90 seconds ...
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bioRxiv - Cancer Biology 2020Quote: ... an equal amount of proteins was electrophoresed on 8 to 15% sodium dodecyl sulfate-polyacrylamide gels prepared in-house and transferred to polyvinylidene difluoride (PVDF) (Bio-Rad, Hercules, CA, USA; Cat# 1620177) membrane at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins (20-30 μg) of the tissue protein samples were subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad mini-PROTEAN TGX Gels, # 4561044) and transferred to polyvinylidene fluoride (PVDF ...