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Citations for Bio-Rad :
151 - 200 of 216 citations for 7 Aminodesacetoxycephalosporanic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Isolated RNA was used to synthesize complementary deoxyribonucleic acid (cDNA) with the iScripttm cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... in 1% (v/v) acetic acid] for 1 min and photographed using Gel Doc XR system (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Supernatant was collected and the protein sample concentration was calculated using a bicinchoninic acid (BCA) assay (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Approximately 50 ng cDNA from each sample was mixed with gene-specific primers (Supplementary Table 7) and SsoAdvanced™ universal SYBR® Green supermix (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with KOD SYBR qPCR Mix (TOYOBO) and primers (Supp Table 7) using the CFX Connect Real-Time PCR Detection System (Bio-Rad; Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... total proteins were quantified in paw extracts using the Bicinchoninic acid technique (BCA) and 75-100µg of proteins were loaded on 12% polyacrylamide gels (Biorad). The migration step was followed by a liquid transfer on PVDF membranes (100V 40min ...
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bioRxiv - Biochemistry 2020Quote: ... and the quantity of radiolabel included into the acid insoluble fraction was analysed using Quantity One software (Bio-Rad). The quantitative data were analysed using MS Excel 2010 and presented on histograms as the Mean ± SD.
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bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
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bioRxiv - Microbiology 2019Quote: ... The gels were then fixed in 12% trichloroacetic acid for 1h and stained with QC Colloidal Coomassie stain (BioRad). Molecular weights were estimated using a calibration curve (Log10 MW vs Rf ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: LiRecT protein was buffer exchanged into 100 mM ammonium acetate pH 7 (unadjusted) using Micro BioSpin P6 spin columns (Bio-Rad Laboratories, Hercules, CA, USA). All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... before transfer onto a nitrocellulose membrane (Transblot Turbo Transfer Pack Midi 0.2 μm) for 7 min using the Transblot Turbo Transfer System (BioRad, program: Quickblot Mixed MW, Midi Gel). Membranes were incubated for 1 hour in blocking buffer (10% BSA ...
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bioRxiv - Physiology 2022Quote: ... Proteins were then either semi-dry transferred onto a polyvinylidene difluoride membrane at 1.3 A and 25 V for 7 min (Trans-Blot® Turbo™ Transfer System, Bio-Rad, Hercules, California, USA) or wet transferred onto nitrocellulose membrane at 100 V for 90 min (i.e. ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMarkTM Microplate Absorbance Reader (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
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bioRxiv - Microbiology 2019Quote: Aliquots of extracted nucleic acid from plasma were processed in triplicate (1.5ul per replicate) following the recommended workflow (WX200 ddPCR system, Bio-Rad). Custom PrimeTime (IDT ...
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bioRxiv - Physiology 2020Quote: ... Samples and standards were incubated with bicinchonic acid for 30 minutes prior to measuring absorbance as 540 nm using an iMark microplate absorbance reader (BioRad).
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bioRxiv - Microbiology 2022Quote: ... A Bio-Rad Aminex HPX-87H HPLC column for organic acid analysis (300 × 7.8 mm; Bio-Rad Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were Coomassie stained and destained with 10% acetic acid prior to imaging on a ChemiDoc MP imaging system (BioRad).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped using glacial acetic acid and the N-glycans desalted sequentially using strong cation exchange (AG 50W X8, Bio-Rad), C18 and porous graphitised carbon (PGC ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Microbiology 2022Quote: ... Reaction was developed using a freshly prepared TMB substrate solution and stopped with 1M Sulfuric acid before determining the OD450 using a microtiter plate reader (iMark mic, Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then stained with Coomassie Blue solution, followed by de-staining (Methanol, Acetic Acid solution) and visualised by using Gel Doc™ XR+ system (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
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bioRxiv - Biophysics 2022Quote: ... Gels were fixed for 10min (50% methanol, 10% acetic acid) and stained with Coomassie Blue for imaging on a gel doc (Bio-Rad). Band intensities were quantified using Image Lab (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped by 10% acetic acid and the gel was washed with water for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMark™ Microplate Absorbance Reader (Bio-Rad).
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bioRxiv - Evolutionary Biology 2021Quote: ... and these values were converted to metabolite concentrations (in mM) using organic acid and sugar standards (from BioRad or made in-house). Citrate ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were run with 10 mM sulfuric acid at 0.6 mL/min flow rate on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3-HB and n-butanol were separated with 5 mM sulfuric acid as the mobile phase and one of two column conditions: (1) an Aminex HPX-87H or Fast Acids anion exchange columns (Bio-Rad Laboratories) at 35 or 55 °C and a flow rate of 0.6 ml min-1 or (2 ...
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bioRxiv - Immunology 2021Quote: ... the enzyme reaction was stopped with 50 μL of 1 M sulfuric acid per well and the absorbance was measured in Bio-Rad Model 550 microplate reader (Bio-Rad Laboratories). Sera were assayed in duplicates and antibody titer represents the last reciprocal serum dilution above blank.
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bioRxiv - Molecular Biology 2023Quote: The interaction between nucleic acids (ligands) and the proteins (analytes) were measured by the surface plasmon resonance (SPR) technique using a ProteOn XPR36TM (Bio-Rad Laboratories). NLC Sensor chips precoated with NeutrAvidin (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% (v/v) acetic acid) and imaged using the Molecular Imager Gel Doc XR System and the Quantity One Software Package (Bio-Rad, Germany), or they were used for immunodetection ...
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bioRxiv - Microbiology 2021Quote: ... The bound dye in the wells was eluted with 33% acetic acid and the absorbance of the solution was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). To assess the individual bacterial population during growth ...