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Citations for Bio-Rad :
151 - 200 of 5601 citations for 7 3 2 3 5 dihydroxyphenyl 2 hydroxyethyl amino propyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione monohydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... R primer: 5′-GTACCACCATGTACCCAGGC-3’) cDNA were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Cat.:# 170882) in a qPCR detection system (LightCycler LC480 ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... build 7 (BioRad). Primary antibodies were as follows ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (0.2 µm pore size) using the Trans-Blot® Turbo™ semi-dry transfer system (7 min mixed MW MIDI program) (Bio-Rad). After blocking in a 2.5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-Tubulin (rat, YL1/2, MCA77G, Bio-Rad, 1:2000 (Figure 5) or 1:10000 (other figures) ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected with 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad). The CA levels from cells were normalized relative to GAPDH levels ...
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bioRxiv - Neuroscience 2019Quote: ... then 3 washes in 1% milk were performed prior to imaging the blot with ECL (BioRad Clarity #1705060). Membrane was stripped using the GM Biosciences One Minute Advance Stripping buffer #GM6031 ...
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... and the sample was heated to 90°C for 5 minutes before loading of 3 pmol onto a 4-15% Mini-PROTEAN TGX Stain-Free Precast Gel (Bio-Rad), alongside PageRuler Prestained Protein Ladder (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in TBS-T/milk for 45 min and washed 3 × 5 min with TBS-T before detecting using a Chemidoc imager (Bio-Rad). NearIR-conjugated secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were washed in 1X PBS with 0.1% Tween 3 times for 5 minutes each before scanning using the ChemiDoc™ MP Imaging System (Bio-Rad). Note ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times for 5 min with TBS and were subsequently incubated with Clarity Western ECL substrate working solution (Bio-Rad) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
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bioRxiv - Neuroscience 2019Quote: ... capacitance of 3 μF and a resistance of 400 Ω (Gene Pulser II (BioRad)) ...
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bioRxiv - Genomics 2020Quote: Lysates were separated by SDS-PAGE on 3-8% Tris-acetate gels (BioRad #3450129) for 2.5 hours at 150V ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 µl was used for qPCR with iTaq Universal SYBR Green Supermix (BioRad). Relative levels of RNA were calculated by the ΔΔCt method using GAPDH as an internal control ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose membrane was soaked (blocked) in 3% milk (dry milk from Bio-Rad) solution in 1x PBS following by incubation with appropriate primary antibody solution (1:1000 dilution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... washed 3 times and developed with ECL reagent (Bio-Rad Laboratories, Hercules, CA, USA). Invertase blots were prepared similarly with an anti-invertase antibody and anti-goat IgG HRP ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
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bioRxiv - Evolutionary Biology 2023Quote: ... kinase-dead extracts were incubated 3 consecutive times with protein A-beads (Bio-Rad) previously coated with the anti-B55δ antibody (1 hour incubation at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Blotting membrane was primed for 3 min prior in cold transfer buffer (BIORAD 10026938), according to manufacturer specifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Following separation via SDS-PAGE (12%, Mini PROTEAN 3 System, Bio-Rad, Hercules, CA), proteins were transferred to PVDF membrane for 1hr using a Genie electroblot chamber (Idea Scientific ...
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bioRxiv - Microbiology 2023Quote: ... samples were run through Criterion XT Tris-acetate precast gels (3-8% gradient) (Biorad) in a XT tricine buffer (Biorad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were loaded onto a 3% agarose gel and visualized on a Chemidoc (BioRad). Bands were densitometry quantified by ImageJ and the percentage of methylated DNA was calculated over the total intensity of all the quantified bands.
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 μg of the protein samples were prepared with 4X Laemmli loading buffer (Biorad) and denatured at 95°C for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... 3) live cell populations using live-dead exclusion dye (Zombie Aqua, #423101, Bio-Rad); 4 ...
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bioRxiv - Immunology 2024Quote: ... Samples were diluted in Laemmli sample loading buffer supplemented with |3-mercaptoethanol (Bio-Rad), heated for 5 min at 95°C ...
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bioRxiv - Bioengineering 2020Quote: ... anti-YL1/2 (1:1000, BioRad) primary antibodies ...
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bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... and supplemented with 5% 2-mercaptoethanol (Bio-Rad Laboratories) for 8 minutes at 100 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Laemmli sample buffer (Bio-Rad #1610747, 5% 2-mercaptoethanol) was added to 20 µg protein and samples were boiled at 95°C for 5 minutes before being loaded onto a 10% SDS-polyacrylamide gel (Bio-Rad #4568034) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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bioRxiv - Cell Biology 2021Quote: ... for 7 minutes (BioRad mixed molecular weight pre-set programme) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mM TEMED (BIORAD) and run from 10X stock of cathode buffer (1 M Tris pH 8.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Electrofocusing was performed in glass capillaries with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (#163-1112, #163-1192, Bio-Rad, USA). The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1 ...
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bioRxiv - Biochemistry 2023Quote: ... pre-run for 1 hour at 4 ° C and run for 1 hour at 120 volts on Mini-Protean 3 gels (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... This step was repeated for 3 times and the samples were concentration-matched at OD500nm = 10 and mixed 1:1 with 2x Laemmli buffer (Bio-Rad), containing SDS and 2-mercaptoethanol ...
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bioRxiv - Immunology 2019Quote: Samples were diluted 1 in 3 in HBSPE and run through Bio-Gel® P-30 (Bio-Rad, UK) polyacrylamide gel spin columns to minimise non-specific binding ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-Rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...