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Citations for Bio-Rad :
1901 - 1950 of 3233 citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... anti-CD31 (rat monoclonal clone ER-MP12, #MCA2388T, RRID: AB_1101899, BioRad Laboratory; 1:200 for IHC), anti-Nestin (chicken polyclonal ...
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bioRxiv - Molecular Biology 2023Quote: ... A 20 µL ddPCR reaction mixture contained 1 × ddPCR master mix (Bio-Rad, Hercules, CA, USA), 0.9 µM primers ...
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bioRxiv - Physiology 2024Quote: ... on 1:25 diluted cDNA with the CFX96 real-time system (BIORAD, Laboratories GmbH, Vienna, Austria). Results are expressed as 2-ΔΔCt (Livak method ...
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bioRxiv - Microbiology 2023Quote: ... one-step RT-ddPCR (1-Step RT-ddPCR Advanced Kit for Probes, Bio-Rad, Hercules, CA) was used to determine absolute quantification of N and S vRNA copy numbers in culture supernatants and cell lysates using the OC43 ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.5–1 μg RNAs were reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad). Specific primers ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 1 hr with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) or anti-mouse (GE Healthcare UK Limited NA931V ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was obtained from 1 ug RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). CBX7 and GAPDH qPCR was performed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated from 1 µg total RNA using iScript cDNA Synthesis Master Mix (Bio-Rad). cDNA was then diluted 1:20 for rRNA intermediates and 1:100 for mature rRNAs ...
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bioRxiv - Microbiology 2023Quote: ... followed by 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 3% milk in PBS-T and developed as described above.
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bioRxiv - Physiology 2024Quote: ... exposed to a primary rat anti-mouse F4/80 monoclonal antibody (1/200) (MCA497G, BioRad, USA). A rabbit anti-rat immunoglobulin (1/200 ...
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bioRxiv - Cell Biology 2024Quote: ... ATG16L1 P-S278 antibody was diluted at 1:4000 in EveryBlot blocking buffer (Bio-Rad, 12010020) and incubated overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg of RNA was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (BioRad). QPCR was performed with cDNA using the SsoAdvanced Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... and applied to a 5 mL prepacked Mini ceramic hydroxyapatite (CHT) type 1 column (Bio-Rad). The flowthrough was collected and purified by SEC using a HiLoad 16/600 Superdex 200 (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of total RNA was reverse-transcribed with iScriptTM cDNA Synthesis Kit (BIO-RAD, 1708891). Quantitative PCR were performed using Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... the membrane was incubated with 1:10,000 dilution of HRP-conjugated anti-rabbit IgG (BioRad, 1706515) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and Goat pAB to RB IgG HRP (Abcam were both used at 1:10000. Subsequently BioRad Clarity Max Western ECL Substrates were applied and the final results were imaged using a GelDoc.
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with tris buffered saline (TBS) with 1% casein blocker (Biorad, Cat. No: 1610782) for one hour ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µg of RNA was used to synthesize cDNA with iScript™ cDNA Synthesis Kit (Biorad), and 100 ng cDNA was used as a template for the reaction ...
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bioRxiv - Biochemistry 2024Quote: ... 1-20 μg of total protein was resolved on a 4-20% polyacrylamide gel (BioRad, 4561096) at 100 V for 60-90 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... followed in each case by anti-mouse anti-IgG secondary antibody (1:5,000 dilution; Bio-Rad). Immunofluorescent microscopy of T ...
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bioRxiv - Microbiology 2024Quote: ... the film was incubated with 1:2500 goat anti rabbit HRP antibody (BIO-RAD cat. #1706515) for an hour before visualization.
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bioRxiv - Physiology 2024Quote: ... the blots were washed and incubated with a 1:3000 dilution of goat anti-rabbit (BioRad) or anti-mouse secondary antibodies (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed with TBST and incubated with HRP conjugated secondary antibodies (Bio-Rad, 1:10,000) at room temperature for 1h for ECL chemiluminescence detection system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was done in a 1 mm cuvette using a Gene Pulser Xcell (BioRad, München, Germany) with a constant time protocol with input parameters 1.5 kV ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, cat#1708841), then diluted 10-fold and used directly as the template for quantitative real-time RT-PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: ... Detection via secondary antibody was done with goat α-rabbit mAb (1:5000) conjugated to HRP (Biorad). Immunoblots were developed using the SuperSignal West Femto (Thermo ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM pH 8.0 by two cycles of centrifugal gel filtration (Biospin mini columns, 6,000 MWCO, Biorad). The nsp’s were buffer-exchanged into 250-500 mM NH4OAc ...
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bioRxiv - Cell Biology 2020Quote: ... StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500, Bio-Rad, Cat# 12005870, RRID: AB_2884949) and hFAB Rhodamine Anti-Tubulin Antibody (1:2500 ...
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bioRxiv - Microbiology 2020Quote: ... and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). % recovery was calculated as 2 raised to the adjusted input Ct minus IP Ct multiplied by 100 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used for western blot and IF include: goat anti-rabbit HRP (1:10,000, BioRad), goat anti-mouse HRP (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as Plet1 (1:200; Nordic MUbio MUB1512P conjugated to Bio-Rad ReadiLink 633/655 1351005). Single-cell suspensions for ESCs and EPSCs undergoing ES-to-TSC reprogramming as well as control cell lines (ESC/EPSC day 0 samples ...
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bioRxiv - Genetics 2021Quote: ... or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706516). Membranes were incubated in ClarityTM western ECL substrate (Bio-Rad laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 1 µg of RNA was reverse transcribed using iScript reverse transcription supermix (Bio-Rad). Real-time PCR was performed as triplicates on 2 ng of cDNA using SYBR Green master mix (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized from 1 μl/RNA sample using an iScript kit (Bio-Rad, catalog #1708891), and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription using the iScript cDNA synthesis kit (Bio-rad). The gene expression analysis was carried out using the SsoAdvancedTM Universal SYBR Green Supermix (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE and observed by immunoblot using rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000).
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bioRxiv - Molecular Biology 2019Quote: ... and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad) with following parameters ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were assessed using 6% polyacrylamide gel (made with 29:1 Acrylamide/Bis Solution, Bio-Rad) electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Genetics 2019Quote: ... incubated in goat anti-rabbit IgG horseradish peroxidase secondary antibody (1:3000; Bio-Rad, Hercules, CA, USA) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and 1 μg of RNA was transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). Primer pairs MFS17/MFS18 ...
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bioRxiv - Microbiology 2021Quote: ... dried for 1 hour at 70°C on a BioRad Model 583 gel dryer (BioRad, Hercules, CA), and protein visualized with Coomassie staining ...
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bioRxiv - Cancer Biology 2020Quote: ... for smaller pellets 50 µl 1% SDS (Sodium Dodecyl Sulfate, Bio-Rad Laboratories Inc., Hercules, CA, USA) was used.
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of RNA was reverse transcribed into cDNA using the Reverse Transcription Kit (Bio-Rad, USA). qRT-PCR was carried out using SYBR Green (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 min and then imaged for 300 s using the ChemiDoc imaging system (Bio-Rad # 17001401). Dialyzed laminin was adsorbed directly to wells to generate a standard reference curve for quantitation ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 min and then imaged for 120 s using the ChemiDoc imaging system (Bio-Rad # 17001401). Blots were analyzed using ImageLab software (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a one hour incubation in the appropriate secondary antibodies coupled to peroxidase (1:1000, Biorad). Proteins were detected by enhanced chemiluminescence detection (ECL ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes for 1 hour using the Trans-Blot turbo (BioRad) system using standard conditions ...