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Citations for Bio-Rad :
1851 - 1900 of 1949 citations for 6 Chloro 3 2 chloroethyl 1H pyrrolo 2 3 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Captured elution fraction from the Strep-Tactin-affinity purification were loaded onto a anion exchange column (UNO Q-6, Biorad) with a flowrate of 4 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Biochemistry 2022Quote: ... the enzymes were buffer exchanged into their respective reaction buffers above using a Micro Bio-Spin 6 size exclusion spin column (Biorad) to remove glycerol and other ion suppressing constituents ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
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bioRxiv - Developmental Biology 2019Quote: ... Alcian Blue was extracted by 6 M guanidine hydrochloride solution and measured by optical density at 600 nm (O.D. 600) using spectrophotometer (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Neuroscience 2019Quote: ... Serum was isolated and IL-6 levels were measured using a Bio-Rad Luminex system (Bio-Rad, Laboratories, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE buffer (pH 8.0) and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Plant Biology 2023Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE (pH 8.0) buffer and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Biochemistry 2023Quote: ... The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad). Molecular weight of standard was as follows ...
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bioRxiv - Biochemistry 2023Quote: ... CsgA and CsgAslowgo samples were buffer-exchanged into MS-compatible buffer (150 mM Ethylene diamine diacetate pH 6.3, 10mM TMAO) using Micro Biospin 6 desalting columns (Bio-Rad) to a final protein concentration of 3-5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Biochemistry 2024Quote: AcrAS was buffer exchanged into a volatile solution (100 mM ammonium acetate, pH 6.0) using a centrifugal exchange device (Micro Bio-Spin 6, Bio-Rad) according to manufacturer’s instructions native MS experiments were performed on a Synapt G2-Si mass spectrometer (Waters) ...
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bioRxiv - Cancer Biology 2024Quote: As previously described,(6) we used the Bio-Plex Pro Human Chemokine 40-plex Panel (Bio-Rad Laboratories, Hercules, California) on a Luminex 200 System to measure plasma cytokines ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were run at 6 W/gel and followed by Cy3 visualization using the Molecular Imager PharosFX system (BioRad).
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bioRxiv - Biochemistry 2024Quote: Purified membrane proteins were buffer exchanged into 200 mM ammonium acetate (pH 7.4 at room temperature adjusted with ammonium hydroxide) supplemented with 2x CMC of the desired detergent using a centrifugal desalting column (Micro Bio-Spin 6 columns, BioRad). For the AmtB-GlnK complex ...
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Bioengineering 2019Quote: Equal volumes of (GT)6-Cy5-SWCNT and FAM-tagged protein at 2X working concentration were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Biophysics 2021Quote: ... beads were washed with wash buffer 6-8 times in poly-prep chromatography columns (cat. no. 7311550, BIO-RAD laboratories Inc). Protein was eluted using Ni-NTA elution buffer (50□mM NaPi pH 7.6 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (20 µg/lane) were electrophoresed on a 6–12% SDS polyacrylamide gel in a Mini-PROTEAN Tetra Cell (Bio-Rad) and subsequently transferred to a nitrocellulose membrane (Sartorius AG ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Cell Biology 2021Quote: ... inside an anaerobic glove-box and the excess DTT was completely removed via a Bio-Gel P-6 DG gel-desalting column (Bio-Rad) in thoroughly de-oxygenated MOPS buffer (20 mM ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 4 µl of the diluted DNA sample was added to 6 µl of a mixture containing SYBR Green Supermix (Bio-Rad) and gene-specific primers (0·4 µM total ...
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bioRxiv - Biophysics 2019Quote: ... 30 μl of purified protein in 0.02% DDM at ∼1 mg/ml was buffer exchanged into 200 mM ammonium acetate pH 7.5 and 0.02% DDM using Biospin 6 gel filtration spin columns (Bio-Rad, Hercules, CA). 2 μl of buffer exchanged ELIC was loaded into a borosilicate capillary emitter (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: A549 or DF-1 cells in 6-well plates were infected with the indicated IAVs and cells were lysed with 2X Laemmli sample buffer (Bio-rad) plus 2% beta-mercaptoethanol at 8 hpi ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and 50 µl of a 10−4 dilution was loaded into selective ura− agar in a 6 cm petri plate and subjected to UVC radiation in a UVP gel Imaging and Documentation device (Bio-Rad) for 15 and 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were dried out at RT and then exposed to a phosphoscreen cassette (GE) for 6 days for further scanning in a phosphoimager analyzer (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... chromosomal DNA plugs were prepared and separated on 1% agarose gel at 6 V/cm for 48 hrs (initial time = 20s, final time = 30s) by using the CHEF DRII apparatus (Bio-Rad), followed by Southern blotting and hybridization with a TRP1 sequence as a probe.
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: Samples were prepared by boiling at 95°C for 6 minutes in lysis buffer supplemented with 4x Laemmli Sample Buffer (Bio-Rad), with 355 mM 2-mercaptoethanol freshly added according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... or immunoprecipitated samples were resolved by SDS-PAGE (6% to 10%) and transferred onto methanol-treated immun-blot PVDF membrane (Bio-Rad). The membranes were incubated overnight at 4°C with corresponding primary antibodies (antibody information ...
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bioRxiv - Immunology 2024Quote: ... The gel was imaged under the Silver Stain setting on Image Lab 6 on a Gel Doc EZ Imager (Bio-Rad). ImageJ was used to quantify band intensities and determine peptide loading efficiencies.