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Citations for Bio-Rad :
1801 - 1850 of 3062 citations for Recombinant Human IIntercellular Adhesion Molecule 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the membrane was incubated with 1:10,000 dilution of HRP-conjugated anti-rabbit IgG (BioRad, 1706515) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... at 18 V for 1 h using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were blocked in 5% skimmed milk in tris-buffer saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg DNase-treated total RNA was then reverse transcribed using iScript cDNA synthesis kit (Biorad). The cDNA was subsequently used for quantitative ...
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bioRxiv - Biophysics 2023Quote: ... 20 mM imidazole and 1 mM DTT) and transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... goat anti-rabbit IgG (H + L)-HRP conjugate (170-6515, Bio-Rad, CA, USA; 1:2000), and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were probed with either secondary antibodies goat anti-mouse-HRP (Bio-Rad, 1706516, 1:5000) or goat anti-rabbit-HRP (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The pellet re-suspended in 1 x Tris buffer saline (TBS) with 0.1% SDS (BIO-RAD) was subjected to a brief sonication on ice for shearing the genomic DNA using Diagenode Bioruptor Plus sonicator (Diagenode Inc.) ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... membranes were blocked at room temperature for 1 h with 1X casein blocking solution (Bio-Rad) (#1610782) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cDNA was synthesized from 1 μg of RNA using iScript Reverse Transcription Supermix (Biorad, USA) as per the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.5–1 μg RNAs were reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad). Specific primers ...
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bioRxiv - Neuroscience 2023Quote: ... anti-CD31 (rat monoclonal clone ER-MP12, #MCA2388T, RRID: AB_1101899, BioRad Laboratory; 1:200 for IHC), anti-Nestin (chicken polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... one-step RT-ddPCR (1-Step RT-ddPCR Advanced Kit for Probes, Bio-Rad, Hercules, CA) was used to determine absolute quantification of N and S vRNA copy numbers in culture supernatants and cell lysates using the OC43 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were run on 8% SDS-PAGE gel for 1 hour at 175V (Tetra cell, BioRad). Following migration ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were incubated with horseradish-peroxidase-conjugated anti-rabbit (Bio-Rad, 1721019, 1:2000, RRID: AB_11125143) or anti-mouse (SouthernBiotech ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was obtained from 1 ug RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). CBX7 and GAPDH qPCR was performed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... P-ATG16L1-S278 antibody was diluted at 1:4000 in EveryBlot blocking buffer (Bio-Rad, 12010020) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... at 100 V for 1 h in a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad) kept at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used: goat α-mouse (1:10 000, Bio-Rad, 170-6515), donkey α-goat (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... and secondary antibody goat anti-rabbit IgG (H+L)-HRP-conjugate (1:10,000 dilution, Bio-Rad). Image lab (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used in this study: sheep polyclonal anti-TGN46 (#AHP500G, BioRad, 1:2000), rabbit polyclonal LRBA antibody (#HPA023567 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 1 hr with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) or anti-mouse (GE Healthcare UK Limited NA931V ...
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bioRxiv - Microbiology 2023Quote: ... followed by 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 3% milk in PBS-T and developed as described above.
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated from 1 µg total RNA using iScript cDNA Synthesis Master Mix (Bio-Rad). cDNA was then diluted 1:20 for rRNA intermediates and 1:100 for mature rRNAs ...
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bioRxiv - Molecular Biology 2023Quote: ... A 20 µL ddPCR reaction mixture contained 1 × ddPCR master mix (Bio-Rad, Hercules, CA, USA), 0.9 µM primers ...
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bioRxiv - Physiology 2024Quote: ... on 1:25 diluted cDNA with the CFX96 real-time system (BIORAD, Laboratories GmbH, Vienna, Austria). Results are expressed as 2-ΔΔCt (Livak method ...
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bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 hour at RT with appropriate HRP-conjugated secondary antibodies (Bio-Rad) diluted 1 in 3,000 in TBST with 5% milk ...
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bioRxiv - Neuroscience 2024Quote: ... 1/6 of the total amount was loaded on a 4-20% SDS-PAGE (Bio-Rad), while the remaining sample was used for detecting NMNAT2 ...
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bioRxiv - Biophysics 2024Quote: ... The coverslips were incubated with the primary antibody [anti-tyrosinated tubulin 1:150 (MCA77G, Bio-Rad)] in blocking solution (10% FBS with 0.05% Tween 20 diluted in cytoskeleton buffer overnight at 4ºC ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... and dried for 1 hour at 80 °C in a gel dryer (Model 583, Bio-Rad). The dried gels were exposed to phosphorimaging plates for 72 hours ...
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bioRxiv - Microbiology 2024Quote: ... 10% glycerol) then boiled with 1/3 volume of 4x Laemmli sample buffer (Bio-Rad, 1610747) for 10 mins ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, cat#1708841), then diluted 10-fold and used directly as the template for quantitative real-time RT-PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: ... Detection via secondary antibody was done with goat α-rabbit mAb (1:5000) conjugated to HRP (Biorad). Immunoblots were developed using the SuperSignal West Femto (Thermo ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM pH 8.0 by two cycles of centrifugal gel filtration (Biospin mini columns, 6,000 MWCO, Biorad). The nsp’s were buffer-exchanged into 250-500 mM NH4OAc ...
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bioRxiv - Cell Biology 2020Quote: ... StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500, Bio-Rad, Cat# 12005870, RRID: AB_2884949) and hFAB Rhodamine Anti-Tubulin Antibody (1:2500 ...
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bioRxiv - Microbiology 2020Quote: ... and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). % recovery was calculated as 2 raised to the adjusted input Ct minus IP Ct multiplied by 100 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used for western blot and IF include: goat anti-rabbit HRP (1:10,000, BioRad), goat anti-mouse HRP (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as Plet1 (1:200; Nordic MUbio MUB1512P conjugated to Bio-Rad ReadiLink 633/655 1351005). Single-cell suspensions for ESCs and EPSCs undergoing ES-to-TSC reprogramming as well as control cell lines (ESC/EPSC day 0 samples ...
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bioRxiv - Genetics 2021Quote: ... or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706516). Membranes were incubated in ClarityTM western ECL substrate (Bio-Rad laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 1 µg of RNA was reverse transcribed using iScript reverse transcription supermix (Bio-Rad). Real-time PCR was performed as triplicates on 2 ng of cDNA using SYBR Green master mix (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized from 1 μl/RNA sample using an iScript kit (Bio-Rad, catalog #1708891), and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription using the iScript cDNA synthesis kit (Bio-rad). The gene expression analysis was carried out using the SsoAdvancedTM Universal SYBR Green Supermix (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE and observed by immunoblot using rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000).
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bioRxiv - Molecular Biology 2019Quote: ... and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad) with following parameters ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were assessed using 6% polyacrylamide gel (made with 29:1 Acrylamide/Bis Solution, Bio-Rad) electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Genetics 2019Quote: ... incubated in goat anti-rabbit IgG horseradish peroxidase secondary antibody (1:3000; Bio-Rad, Hercules, CA, USA) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and 1 μg of RNA was transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). Primer pairs MFS17/MFS18 ...
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bioRxiv - Microbiology 2021Quote: ... dried for 1 hour at 70°C on a BioRad Model 583 gel dryer (BioRad, Hercules, CA), and protein visualized with Coomassie staining ...