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Citations for Bio-Rad :
1801 - 1850 of 7803 citations for Rat Indoleamine 2 3 Dioxygenase 1 IDO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL of the diluted samples were used as input in a ddPCR supermix (Bio-Rad, 1863023). Primers and probes ...
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bioRxiv - Microbiology 2023Quote: ... The relative gene expression was analysed by the comparative 2-ΔΔCt method using CFX 96 software (BioRad). Data were normalized with the endogenous control ...
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bioRxiv - Biochemistry 2022Quote: ... with integration time set to 2 s and imaged using a Gel Doc XR system (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Lysis was performed by directly adding 2x Laemmli sample buffer (supplemented by 2-mercaptoethanol, Bio-Rad Laboratories) at a ratio of 1 µL per 0.1 mg of cell pellets and pipetting up and down ...
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bioRxiv - Biophysics 2022Quote: ... DDM was removed by addition of a 100-fold excess (w/w) of biobeads SM-2 (Biorad) compared to protein for 4 h at 4 °C under gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed in sample buffer (2× Laemmli sample buffer, No. 1610737, Bio-Rad, Hercules, California, USA). Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: ... Captured complexes were dissociated from the beads in the ×2 Laemmli buffer (Bio-Rad Laboratories, Hercules, CA) and analyzed by SDS-PAGE/western blotting using chemiluminescent substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining was done in 2% milk-TBST (+ 0.01% sodium azide) and detected using ChemiDoc (Biorad).
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bioRxiv - Biochemistry 2024Quote: ... Detergent removal was carried out at 4°C via incubation with Bio-Beads SM-2 (Bio-Rad) at a concentration of 200 mg ml-1 for three times with two hours for each time ...
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bioRxiv - Systems Biology 2024Quote: ... containing 2 μl of cDNA template was prepared and analyzed using CFX Connect Real-Time instrument (BioRad) with the following thermocycling conditions ...
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bioRxiv - Plant Biology 2024Quote: ... centrifuged for 2 minutes before running through 4-20% mini Protean TGX Stain Free Gel (BioRad, USA). The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4-15% Tris-Glycine gel (Bio-Rad Cat#5678084) using Tris-Glycine running buffer (0.2M Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 or 2 % agarose gel solutions (50 mL total volumes) were prepared with agarose powder (Bio-Rad) using 0.5× TBE buffer (45 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... A hydroxyapatite column was prepared by resuspending 2 g Bio-Gel HTP Hydroxyapatite (Bio-Rad, 130-0420) in 12 ml CDC6 HTP-wash buffer ...
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bioRxiv - Neuroscience 2024Quote: ... or at 100 V for 2 h at 4 °C using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell 170-3930) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was washed three times with T-TBS for 3 x 5 min and incubated for 1 h with 1/1000 solution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (1:1000; Biorad) and washed 3 times with T-TBS and 1 time with TBS ...
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bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres (1000/well) were incubated with serially diluted samples (1:100, 1:1000, 1:10,000) in replicates in Bioplex plates (Bio-Rad) at 4°C for 16hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to 1:1 by adding appropriate amount of ECL (Bio-Rad) color solution in the dark conditions and photographed using an EZ-ECL chemiluminometer Detection Kit for HRP (Biological Industries ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was diluted 1:1 with 2X Laemmli sample buffer (BioRad, 1610737) under reducing conditions and boiled for 5 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:1000 mouse anti-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360), or 1:10,000 mouse anti-actin (C4 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein sample were diluted 1:1 with 2X SDS Laemmli buffer (Biorad) and incubated for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... estrogen receptor (Santa Cruz sc-542, 1:20; BioRad MCA1799T, 1:25), Ltf (Bioss bs-5810 ...
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bioRxiv - Physiology 2021Quote: ... Samples were mixed 1:1 with Laemmli’s sample buffer (BioRad 161-0737) containing 10% 2-Mercaptoethanol (Sigma M3148 ...
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bioRxiv - Genomics 2019Quote: ... RECA-1 (BioRad, MCA970GA), synaptopodin (Progen ...
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bioRxiv - Molecular Biology 2019Quote: ... V5 (BioRad, 1:5,000); α-Tubulin (Upstate Biotech ...
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bioRxiv - Microbiology 2019Quote: ... (1:2000, Biorad, 1706516) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... 1% SDS (Bio-Rad), 0.005% Bromophenol Blue (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT (BioRad), 20 mM 12.5% glycerol (Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT (BioRad), 15 mM EGTA (Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... aromatase (1:1000, BioRad), or loading control GAPDH (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... 1:2000 (#MCA4740, BioRad), mouse anti-mCherry ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... from ART or ART + GYY4137 treated groups were harvested to isolate total RNA using Qiagen RNAeasy isolation kit and 200 ng of total RNA was reverse transcribed (iScript™cDNA synthesis kit, Bio-Rad). Reverse transcribed cDNA was diluted 10-fold and amplified using primers against HIV LTRs and seminested – PCR was performed using primers and probe listed in Table S6 ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Physiology 2020Quote: ... RNA of treated cells was isolated using EZ-RNA isolation kit (Biological Industries Beit Haemek, Israel) and cDNA was synthesised using iScript cDNA synthesis kit (Bio-Rad, UK). Real-time qPCR analyses were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... we purified total RNA using the Qiagen RNeasy Mini kit (Cat#74106) and made cDNA using iscript cDNA synthesis kit from Biorad (Cat#1725036), followed by a Taqman assay to detect AHR transcription (Hs00169233-m1 for AHR expression and Hs0442063-g1 for GAPDH ...
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bioRxiv - Biochemistry 2021Quote: RT-qPCR was performed using the 2019-nCoV RUO Kit (Integrated DNA Technologies, #10006713) in combination with the iTaq™ Universal SYBR® Green One-Step Kit (BioRad). Each 20 μl reaction contained 10 μl 2X iTaq universal SYBR® Green reaction mix ...
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bioRxiv - Evolutionary Biology 2021Quote: RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Cat# 74104) and cDNA was prepared using the iScript cDNA Synthesis Kit (Biorad, Cat# 1708890). Assays were done in triplicate using Express SYBR GreenER master mix (Invitrogen ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Whole-larvae cDNA was generated by isolating total RNA from 3–5 dpf zebrafish larvae using the E.Z.N.A Total RNA kit (Omega, Bio-Tek INC, Cat# R6834-01) and reverse transcribing using the iScript cDNA synthesis kit (BioRad, Cat# 1708891). Fragments of coding sequences of these genes were amplified by PCR using gene-specific primers as follows:
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR (qPCR) was performed with primer sets corresponding to primer list (Table 3) and using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 172-5125) on an Applied Biosystems 7500 Fast Real-Time PCR system or ThermoFisher Scientific QuantStudio 3 or 5 Real-Time PCR system ...
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bioRxiv - Biochemistry 2022Quote: ... The plate was centrifuged at 2000rpm (3 minutes at room temperature) and placed in the CFX Touch™ Real Time PCR Detection System (Bio-Rad; Hercules, California, USA) set for an initial denaturation (95°C ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1:100 or BD Pharmingen 564766, 1:500, CD31: abcam ab28364, 1:100, vimentin: abcam ab92547, 1:100, CD68 [FA-11]: Bio-Rad MCA1957 ...
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bioRxiv - Cancer Biology 2021Quote: ... a DNA extraction kit (InstaGene Matrix-Bio-Rad – USA) is utilize zed for this purpose ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized using iScript cDNA Synthesis Kit (BioRad). qPCR was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... before reverse transcription using iScript cDNA Synthesis kit (BioRad). Real-time PCR was performed with diluted cDNAs in a 15 μL reaction volume using Kapa SYBR Fast qPCR mix in duplicates and analyzed using the CFX96 Touch Real-Time PCR Detection System and computing ΔΔCq using the CFX96 software ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were developed with an ECL reagent kit (BioRad). Western blots were quantified using Fiji software (“Gels” command).
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bioRxiv - Genomics 2021Quote: ... whereas a KRAS mutation screening kit (Biorad, Hercules, CA) was applied to patient M10 ...