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Citations for Bio-Rad :
1801 - 1850 of 3083 citations for 7 Chloro trans 2 hepenoic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The high molecular weight protein GCN1 was transferred using the Trans-Blot Turbo Transfer System (Bio-Rad, Cat# 1704150 and Cat# 1704158). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... 25-40μg of protein was run on 7.5-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred using the Trans-Blot Turbo system (Biorad, Hercules, CA) to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... The gel was subject to 100 V for 3.5 h and the gel underwent semi- dry transfer by a Trans-Blot® Turbo™ Transfer System (Bio-Rad) onto a 0.2 µm polyvinylidene fluoride membrane at 1.3 A for 11 min ...
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bioRxiv - Pathology 2023Quote: ... and the other one was subjected to the western blot assay using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (Bio-Rad Laboratories, CA). After transferring the total proteins to the PVDF membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then separated on 10-12% SDS-PAGE gels and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans Blot Turbo™ transfer system (Bio-Rad). Membranes were blocked and primary antibodies were diluted in 2% BSA in Tris-buffered saline (TBS) ...
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bioRxiv - Neuroscience 2023Quote: ... and transferred to polyvinylidene difluoride membranes using a semi-dry Bio-Rad transfer machine (Trans-Blot™ Turbo TM Transfer System) with 1X Transfer Buffer (Bio-Rad) at 25V for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... the immunoprecipitated and input proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes via a Trans-Blot Turbo Transfer System (Bio-Rad, USA). After blocking the membranes with a solution of 5% skim milk prepared in PBST with 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was resolved using SDS-PAGE and transferred to the nitrocellulose membrane using a transfer system (Trans-Blot Turbo; Bio-Rad Laboratories). The membrane was blocked using 5% skimmed milk (American Bioanalytical ...
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bioRxiv - Neuroscience 2023Quote: ... We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins in the gel were transferred to a nitrocellulose membrane by Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories) at 25 V ...
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bioRxiv - Molecular Biology 2023Quote: ... and the proteins were transferred onto nitrocellulose membranes (0.2 µm pore size) by blotting performed using the Trans-Blot Turbo Transfer System (Bio-Rad; Hercules, USA) essentially according to the manufacturer (25V/1,3 mA ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then blotted onto a PVDF membrane (pore size 0.2 µm) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) and the “Mixed MW” protocol (7 min blotting time) ...
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bioRxiv - Immunology 2024Quote: ... Lysates were separated by NuPAGE 4- 12 % Bis-Tris Gels (Thermo Fischer Scientific) and transferred to nitrocellulose membranes (Trans-Blot Turbo Transfer Systems (Bio-Rad Laboratories). Membranes were blocked with Odyssey Blocking Buffer (Li-Cor ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Amersham, Cytiva, Marlborough, USA) using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) for 1.5 h at 1.5 mA/cm2 ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Molecular Biology 2024Quote: ... in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific. Proteins were transferred to a 0.2 µm nitrocellulose membrane by Trans-Blot Turbo™ Transfer system (Bio-Rad). Membranes were washed with TBS (50mM Tris pH 7.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% Acrylamide:Bis Acrylamide) for 1 hour and transferred to a nitrocellulose membrane (0.45µm) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF transfer membrane (0.45 μm, ThermoScientific #88518) by using a Trans-Blot®Turbo™ transfer system (Bio-Rad) at a constant current of 2.5A for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... using the Bio-Rad Trans-Blot Turbo Transfer System with 20% Methanol-containing transblot turbo transfer buffer (Bio-Rad; catalog no. 10026938) for 10-15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 M 2-Mercaptoethanol (Bio-Rad), and boiled for 3 min ...
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bioRxiv - Genomics 2019Quote: ... transferred to PVDF membranes using the TurboTransfer System for 7 min at 25 V (BioRad) and blocked for 1 hr with TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... bromophenol blue) was applied onto IPG strip (7 cm, pH 3-10, Bio-Rad, 1632000) for 24 h ...
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bioRxiv - Biophysics 2023Quote: GAGs were purified with a column of 7 mm diameter and 10 cm length (BioRad) packed with Sephadex G25 resin (Sigma-Aldrich # G25150) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated with SDS-PAGE and then later transferred to PVDF membranes by electrophoresis using a Bio-Rad Trans-Blot device (Bio-Rad, Richmond, CA). Blots were blocked for 1 h at room temperature or overnight at 4 °C with 5 % skim milk in Tris-buffer saline with Tween-20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... protein samples were resolved via SDS-PAGE and then transferred onto nitrocellulose membranes by the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Membranes were blocked in 5% non-fat dry milk and hybridized with antibodies in TBST buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Physiology 2019Quote: Cell or tissue protein lysates were separated by electrophoresis on pre-cast 4-15% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (all purchased from Bio-Rad Laboratories), blocked in non-fat milk and incubated overnight at 4°C with primary antibody for HILPDA (Santa-Cruz Biotechnology) ...
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bioRxiv - Biophysics 2019Quote: ... a “sandwich” was prepared with the following successive layers: i) three thicknesses of Mini Trans-Blot ®filter paper (BIO-RAD Cat. #1703932) 7.5 x 10 cm ...
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bioRxiv - Cancer Biology 2019Quote: Proteins were separated by SDS gel electrophoresis and transferred onto a PVDF membrane (Carl Roth, Karlsruhe, Germany) using the semi-dry Trans Blot Turbo (Bio-Rad, Düsseldorf, Germany). Membranes were blocked in 5% non-fat milk powder (Applichem ...
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bioRxiv - Biochemistry 2020Quote: Cell or tissue protein lysates were separated by SDS-PAGE on pre-cast 8-16% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (all purchased from Bio-Rad Laboratories), blocked in non-fat milk and incubated overnight at 4°C with primary antibody for HILPDA (1:750 ...
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bioRxiv - Plant Biology 2020Quote: Proteins were transferred from an SDS-polyacrylamide gel to a polyvinylidene difluoride (PVDF) membrane by electroblotting using a Trans-Blot SD transfer cell (Bio-Rad, www.bio-rad.com) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... size-separated by sodium dodecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE) in 4-20% Criterion TGX Precast gels and transferred to Trans-Blot Turbo Midi PVDF Transfer Packs (Bio-Rad, Hercules, USA). Membranes were blocked in 5% BSA (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... SDS-PAGE and electrically transferred to a PVDF membrane (Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs, Bio-Rad, Hercules, CA, USA) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated on 12% SDS-PAGE gels and transferred to polyvinylidene difluoride membranes (Cytiva Amersham™ Hybond™, USA) with Trans-Blot Turbo Transfer System (BioRad, USA). The membranes were blocked in 5% fat-free milk in phosphate-buffered saline containing 0.2% of Tween-20 (PBST ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred from a 10% SDS-PAGE gel onto a nitrocellulose membrane for a Western blot assay using the Trans-Blot turbo transfer system (Bio-Rad, Hercules CA). Phosphorylated SakA (P- SakA ...
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bioRxiv - Immunology 2022Quote: ... Protein samples were resolved by SDS polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA), followed by blocking for 1 h with 5% nonfat milk in Tris-buffered saline-Tween 20 buffer and probing with antibodies against Strep Tag (SAB2702215 ...
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bioRxiv - Microbiology 2019Quote: Proteins were separated on 4-20% gradient SERVA gels and transferred onto a nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (Bio-Rad). SIGMAFAST™ BCIP®/NBT or SignalFire™ Elite ECL Reagent were used to visualize proteins on C-DiGit Chemiluminescent Blot Scanner (LI-COR Biosciences) ...
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bioRxiv - Pathology 2020Quote: ... LC3-II and p-mTOR were separated by electrophoresis and then transferred onto the nitrocellulose membranes (Bio-Rad; Trans-Blot Turbo Transfer System). After several washes with TBST buffer ...
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bioRxiv - Microbiology 2020Quote: Equal amounts of protein from cell lysates were separated by SDS PAGE and transferred onto a nitrocellulose membrane by a semi-dry transfer method (Trans Blot® SD Semi-Dry Transfer cell, Bio-Rad, USA). Membranes were blocked with 5 % milk solution before incubation with primary antibodies at 1:1000 dilution unless otherwise stated ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were fractionated via 8 ∼ 14% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using a Trans-blot apparatus (Bio-Rad, Hercules, CA). The PVDF membranes were then sequentially subject to blocking ...
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bioRxiv - Biophysics 2021Quote: ... 100 μg of total-proteins was then subjected to electrophoresis in 4-12 % Bis-Tris Plus Gels (Novex®, NW00125BOX) and transferred onto PVDF membrane using a Trans-Blot® Turbo™ Transfer System (BIORAD). After blocking with 5 % non-fat milk ...
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bioRxiv - Immunology 2021Quote: ... Samples were run on SDS-polyacrylamide gels and transferred at 25 V onto nitrocellulose or PVDF membranes using a Trans-Blot® Turbo Transfer™ System (Bio-Rad). The membranes were blocked in either 5% w/v milk or 2.5% BSA (Sigma ...
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bioRxiv - Genetics 2022Quote: ... Aliquots were resolved in 10-12% SDS-polycrylamide gels and transferred to nitrocellulose membranes with a Trans-Blot Turbo Transfer System (Bio-Rad, Madrid, Spain) for blotting ...
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bioRxiv - Physiology 2022Quote: ... per tissue were separated by 4% to 20% sodium dodecyl sulfate– polyacrylamide gel electrophoresis gradient gels electrophoresis (SDS-PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane by semi-dry electrotranfer method (Trans-Blot Turbo Blotting System, Bio-Rad, Hercules, CA). After the transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad, Cat. #1704275). Gels were activated by UV excitation using the Bio-Rad GelDoc XR+ System prior to transfer ...
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bioRxiv - Genetics 2022Quote: ... and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc., CA, USA). Membranes were blocked with 5% non-fat dry milk in PBST for 2h ...
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bioRxiv - Genetics 2022Quote: ... were electrophoresed on a 12% polyacrylamide gel and then transferred on PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated with primary and secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad, Cat #: 1704158EDU) on a Trans-Blot Turbo TM Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred to a 0.2 μm polyvinylidene fluoride (PVDF) membrane using the Trans-Blot® Turbo™ Transfer System (Biorad, Hercules, USA) following the mixed molecular weight (MW ...
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bioRxiv - Neuroscience 2023Quote: ... The resolved proteins were then transferred to polyvinylidene difluoride (PVDF) membranes using semi-dry method on Trans-Blot Turbo Transfer System (1704150, Bio-Rad, CA, USA). Any potential non-specific bindings were blocked by 5% non-fat milk for half hour before incubation with primary antibody ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... Protein samples were run via sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane using BioRad Trans-Blot® Turbo™ Transfer system (BioRad #1704150) and Trans-Blot® Turbo Mini PVDF transfer packs (BioRad #1704156) ...