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Citations for Bio-Rad :
1751 - 1800 of 8683 citations for Recombinant Sortase Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were run on 4-20% precast polyacrylamide gel (4-20% Mini-PROTEAN TGX Precast Protein Gels, BioRad) to separate them and transferred onto PVDF membranes using semi-dry transfer ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Molecular Biology 2022Quote: ... and a calibration curve of protein content was created from the BSA protein standard kit (Bio-Rad Laboratories, CA). An equal amount of protein (50 ng ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were passed through a 26G syringe and protein was quantified by DC Protein Assay (Bio-Rad, cat #5000112). Lysates were diluted to equal volumes and 30 ug protein was loaded per well using the Novex WedgeWell Tris-Glycine Welcome Pack (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 10 min and the protein concentration was determined using a Lowry assay (DC Protein Assay, BioRAD). Nucleic acid coated beads were incubated with 2 mg protein of cell lysates for 60 min and then washed three times with TAP buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Elution of biotinylated protein was performed by heating the beads in 30LμL of 2X protein loading buffer (1610737; BioRad) supplemented with 2LmM biotin + 20LmM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford colorimetric assay (Quick Start™ Bradford Protein Assay Kit; Bio-Rad; 5000201).
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT) until no protein was eluted as monitored by the Bio-Rad protein assay (Bio-Rad, Catalog #5000006). Proteins were eluted with 10 ml elution buffer (50mM Tris-HCl (PH=8),150mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were separated by SDS-PAGE and fluorescent proteins were visualized using the ChemiDocTM MP Imaging system (Bio-Rad). The protein gel was then stained in Coomassie blue solution.
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations were determined by Bradford Assay with the Bio-Rad Protein Assay Dye reagent concentrate (Bio-Rad) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... protein was separated by SDS-PAGE on a 4-15% Criterion TGX pre-cast midi protein gel (Bio-Rad), and transferred to nitrocellulose membrane using a standard tank electrotransfer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Identical amounts of proteins (20-40μg) were electrophoresed on 4–15% Mini-PROTEAN TGX precast protein gels (Bio-Rad). For transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein concentrations of sample supernatants were measured with the Bio-Rad protein assay dye reagent (Bio-Rad, 5000006). Equivalent amounts of protein samples were mixed with Laemmli buffer (100 mM Tris-HCl pH 6.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Identical amounts of proteins (20-40μg) were electrophoresed on 4–15% Mini-PROTEAN TGX precast protein gels (Bio-Rad). For transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... The total protein concentration was determined using Bradford protein assay according to the instructions from the manufacturer (Bio-Rad). POSTN protein expression from the ELISA assay was normalized with the total protein levels ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (30–50 μg) were loaded onto 4%–20% Mini-PROTEAN TGX Precast Protein Gels (BioRad). Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Supernatant was recover and protein concentration was measured using the DC™ Protein Assay Kit II (Bio-Rad, 5000112). Lysates were boiled in the NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Cell Biology 2023Quote: ... 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad). Proteins were transferred to the PVDF membrane using a Trans-blotter (Biorad) ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration das measured of each eluate sample in a 1:20 dilution by Bradford (BioRad protein assay). Per sample ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µg total protein was loaded on AnykD™ Criterion™ TGX™ Precast Midi Protein Gel (Biorad, #5671125) and 60 kDa region was excised from the Coomassie stained gel ...
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bioRxiv - Physiology 2024Quote: ... The total protein content was determined using the colorimetric Bio-Rad Protein Assay Kit II (Bio-Rad Laboratories, CA). Seven dilutions of a protein standard containing 0 to 30 µg/mL of the total protein content were used ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the samples was determined by the BioRad Protein Assay method (BioRad Laboratories, Hercules, CA, USA). For our gels ...
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bioRxiv - Molecular Biology 2024Quote: ... 10µg of total protein was loaded per lane onto a 4-20% Criterion Tris-HCl Protein gel (Bio-Rad) and separated by electrophoresis at 150 V for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each lysate was analyzed using Bio-Rad protein assay reagent according to the manufacturer’s instructions (Biorad). Luciferase units obtained were normalized to the protein content of each well ...
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bioRxiv - Biophysics 2024Quote: ... Total protein concentration in purified microsomes and RyR1 fractions was measured with Quick Start Bradford Protein Assay (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration was determined by Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 20µg of protein was loaded into a 4-15% gradient mini-PROTEAN TGX precast protein gel (4561086, Bio-Rad). Gels were run at 150V in 1x Tris/Glycine/SDS buffer (1610772 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then sonicated and their protein content was quantified using the Bradford protein assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysate and myelin fractions were determined using the DC Protein Assay Kit (Bio-Rad, Munich, Germany) following the manufactureŕs instruction and measured using the EonTM High Performance Microplate Spectrophotometer (BioTek ...
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bioRxiv - Cell Biology 2022Quote: ... 30 µg of total protein per sample was loaded on NuPage 4-12% Bis-Tris Protein gels (Thermo Fischer Scientific) or 4–15% Mini-PROTEAN TGX StainFree Protein gels (Biorad) and run at 200 V as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Each gel was loaded with a Precision Plus Protein™ Dual Color Standards as a protein marker (Bio-Rad, 1610374). Gel electrophoresis was stopped when the Bromophenol blue dye reached the bottom of the gel ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was determined in triplicate with a commercial colorimetric assay (Protein Assay kit-II; Bio-Rad, Gladesville, NSW, Australia), against bovine serum albumin standards (BSA ...
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bioRxiv - Genomics 2019Quote: ... media were collected for insulin determination and rat islet cells were lysed with acid-ethanol (0.2 mM HCl in 75% ethanol) to extract total insulin content or with protein lysis buffer to measure total protein content (Bradford, BioRad). The amount of insulin released in the medium and remaining in the cells was measured by insulin Elisa kit (Mercodia) ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant representing the nuclear fraction was collected and protein concentration of both fractions was determined using the DC protein assay (Biorad). Cytosolic and nuclear fractions were further diluted to the desired protein concentration using the corresponding lysis buffers ...
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bioRxiv - Cancer Biology 2019Quote: ... The volume of the target protein bands and total protein volume of each lane were normalized and quantified using ImageLab software (Biorad). For all figures shown ...
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bioRxiv - Plant Biology 2019Quote: ... The amount of protein recovered after lysis was determined using the Quick Start™ Bradford Protein Assay Kit (Bio-Rad), with bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Trehalase activity was standardized to total protein content using the DC protein quantification technique according to the manufacturer’s recommendations (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... The quantification of protein content in exosome suspensions and whole-cell lysates was undertaken by a modified Lowry assay (DC Protein Assay, BIORAD). For immunoblotting ...
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bioRxiv - Physiology 2021Quote: Equal amounts of skeletal muscle protein extract (45µg) were separated on Mini-Protean TGX precast protein gels (Biorad, CA, USA) and transferred to a PVDF membrane using a Trans-Blot semi-dry transfer system (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Equal protein content for each condition (targeting 10 μg) was run on 10% Mini-PROTEAN TGX precast protein gels (Biorad). After electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... AS and EVs were lysed in RIPA buffer and protein concentration was measured with DC Protein Assay (Biorad, 500-0116), using BSA as standard ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatants were collected and protein concentration was determined using the Bradford-based Bio-Rad protein assay (Bio-Rad). Samples were then denatured and separated using SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted in SDS page loading buffer and separated on 4-15% Mini-PROTEAN TGXTM Precast Protein Gels (BioRad). Samples were processed for immunoblotting or fixed and stained for mass spectrometry using Imperial protein stain (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... The amounts of isolated mitochondria were expressed as the protein concentration using a Bio-Rad protein assay kit (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
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bioRxiv - Genetics 2022Quote: ... Normalization among different technical replicates and conditions was carried out by normalizing the relative luminescence units (RLUs) against total protein content (calculated with the Bradford protein assay, BioRad). Each condition was measured in quadruplicates and each experiment was performed at least twice.