Labshake search
Citations for Bio-Rad :
1751 - 1800 of 6878 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... We visualized the transcription products by electrophoresis in a denaturant gel (23% polyacrylamide, 7 M urea) run at 800V for 2 h (Bio-rad, Hercules, CA, USA).
-
bioRxiv - Cancer Biology 2020Quote: ... Approximately 50 ng cDNA from each sample was mixed with gene-specific primers (Supplementary Table 7) and SsoAdvanced™ universal SYBR® Green supermix (Bio-Rad Laboratories) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with KOD SYBR qPCR Mix (TOYOBO) and primers (Supp Table 7) using the CFX Connect Real-Time PCR Detection System (Bio-Rad; Hercules, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Reaction products were analyzed using denaturing PAGE in a 18% acrylamide gel with 7 M urea and quantified by the ImageLab software (Bio-Rad, Hercules, CA, USA).
-
bioRxiv - Cell Biology 2020Quote: ... The RNA was resuspended in 10 mM Tris pH 8.0 and resolved on a 15% acrylamide Urea-TBE gel (Bio-Rad). A region containing fragments between 17-34 nt (the same size as those isolated for ribosome profiling 32 ...
-
bioRxiv - Molecular Biology 2020Quote: Whole-cell lysates were run on 10 or 15% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (1620097, Bio-Rad) by standard methods ...
-
bioRxiv - Biochemistry 2021Quote: ... 15 μg of proteins were loaded on a 10% Tris–glycine precast SDS-PAGE gel (Mini-PRO-TEAN TGX, BioRad). After a short migration (0.5 cm ...
-
bioRxiv - Genetics 2020Quote: ... The reaction was incubated at 37°C for 15 min and stopped by the addition of 10 μl of TBE-UREA-sample buffer (BioRad). The samples were analysed on a 15% denaturing polyacrylamide gel in 1 × TBE buffer.
-
bioRxiv - Biochemistry 2021Quote: ... All binding reactions were mixed well and incubated at room temperature for at least an hour before mixed with 6x native RNA loading dye (15% Ficoll, 0.02% xylene cyanol, 1xPBS) and loaded onto custom-poured mini gels (BIO-RAD). All gels were run at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... Proteins from bacterial extracts were separated by electrophoresis on 15% polyacrylamide gels and transferred onto nitrocellulose membranes using a wet blotting apparatus (BioRad). Membranes were blocked with 5% milk in PBST (Phosphate buffer saline ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated for 15 min at 30 °C and then applied to a Bio-Gel A-1.5m (Bio-Rad) resin packed in a 0.7×15cm Flex-Column (Kimble) ...
-
bioRxiv - Plant Biology 2020Quote: ... The gel blot was done as described before (Loizeau et al., 2014): 10 μg total RNA was separated on 15% polyacrylamide TBE urea gels (Biorad) and transferred in a wet-blot setup with 0.5 TBE buffer to a Hybond-N membrane (GE-Healthcare) ...
-
bioRxiv - Systems Biology 2021Quote: ... followed by 15 s at 95 °C and 60 s at 58 °C for 40 cycles using Biorad CFX384 thermocycler (Biorad). The mRNA levels of genes encoding cytokine expression were normalized relative to the mean levels of the housekeeping gene and compared using the 2−ΔΔCt method as described previously2 ...
-
bioRxiv - Cell Biology 2020Quote: ... samples were loaded onto Mini-PROTEAN TGX Pre-cast Stain-Free gels (5-15%, Bio-Rad Laboratories, Hercules, CA, USA) and total protein was visualised post-transfer to PVDF membranes on ChemiDoc Touch Imaging System ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated via SDS-PAGE in 15% acrylamide gels and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo Transfer System (BioRad) and PVDF transfer packs (BioRad) ...
-
bioRxiv - Physiology 2021Quote: ... Proteins (50 μg) were loaded onto 12–15% SDS-PAGE gels using a Mini-Protean apparatus (Bio-Rad, CA, USA) and transferred onto nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Physiology 2020Quote: ... 10-25 µg of total protein was loaded in a 15-well pre-casted gradient gel (Bio-rad, 456-8086). After electrophoresis ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Then proteins were transferred from the gel to polyvinylidene fluoride (PVDF) membrane for 52 minutes at 15 V in Trans Blot Semi Dry Transfer Cell (BioRad). The membrane was cut horizontally along the 25 kDa marker band to allow for probing with two different antibodies ...
-
bioRxiv - Molecular Biology 2020Quote: ... gels were submerged into 0.5 µg/ml ethidium bromide in water for 15 minutes and imaged with Gel Doc XR+ system (Bio-Rad). Banding pattern of Mlh1+/− samples were compared to the controls to identify the allele with and without Mlh1 promoter methylation.
-
bioRxiv - Biochemistry 2021Quote: ... The gel was stained 15 minutes in 1x SYBR gold in TBE with gentle shaking and visualized using a Gel Doc EZ Imager (Biorad). Fragments between 26-34 nucleotides were excised and purified by Small-RNA PAGE recovery kit (Zymo Research ...
-
bioRxiv - Cell Biology 2021Quote: ... ECM and RPE cell lysate (15-20 μg protein) were run separately on precast Mini-Protean TGX gels (Bio-Rad) and transferred onto PVDF membrane (Immobilon) ...
-
bioRxiv - Cancer Biology 2021Quote: ... thawed and centrifuged at full speed in an Eppendorf microcentrifuge for 15 minutes and protein content was assessed by Bradford assay (Biorad). Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2020Quote: ... proteins in loading buffer were boiled for 5 min before loading on to 12-15% SDS-PAGE gels using the Mini-Protean III gel system (BioRad). A protein transfer apparatus (BioRad ...
-
bioRxiv - Genomics 2021Quote: ... Antibodies were affinity purified using either the peptides linked to SulfoLink beads (Pierce) or the Ascaris proteins (without GST) linked to a mix of Affigel-10/15 (BioRad).
-
bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 15 minutes in 1x TBST solution and incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... diluted in PBS for 15 mins and then permeabilized with 0.1% Triton X-100 (PBST, BioRad Laboratories, Hatfield, PA, USA) in PBS for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... were resolved on SDS 8-15% polyacrylamide gels and transferred onto nitrocellulose membranes using the Trans-Blot turbo transfer system (BioRad). Membranes were blotted with the primary antibodies against ADMA (Epicypher ...
-
bioRxiv - Synthetic Biology 2022Quote: ... the hybrid RNA/DNA origami was run on 1.5% agarose gel in TAE buffer (75 mL were poured into a 15 cm x 10 cm gel tray, Biorad) and analysed by in-gel imaging using the specific dye DFHBI-1T as described above with few modifications due to the different gel composition (agarose instead of polyacrylamide ...
-
Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
-
bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 35 °C to 79°C at a rate of 0.3 °C per 15 s in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Fluorescence intensity was determined using excitation (λem=525/20 nm ...
-
bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 sec at 95°C and 30 sec at 60°C in a CFX96 cycler (Biorad). Data were normalized to the transcript encoding PP2A subunit A3 (At1g13320 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10µl of dyed samples were loaded onto a prerun (200V for 60 min) 15% TBE-Urea PAGE gel (BioRad 4566055) and resolved at 200V for 35 min ...
-
bioRxiv - Plant Biology 2024Quote: ... using 45 cycles of 15 s at 95°C and 30 s at 60°C in a CFX96 cycler (Biorad). Primers are listed in Additional file 1.
-
bioRxiv - Neuroscience 2023Quote: ... and the samples were boiled in SDS loading buffer and loaded onto 15% Tris-HCl pre-cast gels (Bio-Rad) for Western blotting analysis.
-
bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 50°C and 5 minutes at 85°C in T100 Thermal Cycler (1861096, Bio-Rad). Real-time qPCR reactions were performed using SsoAdvanced Universal SYBR® Green Supermix (1725274 ...
-
bioRxiv - Neuroscience 2023Quote: ... The lysates were then centrifuged at 13,000 rpm for 15 min and protein amounts in the supernatants were measured by DC Protein assay (Bio-Rad). Proteins (10-20 μg ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were separated by dual Tris-glycine SDS-PAGE (lower quarter 15%, upper ¾ 10% acrylamide gel and transferred to a P-type PVDF membrane using the BioRad wet transfer system ...
-
bioRxiv - Biochemistry 2022Quote: ... The other two lanes were incubated with 1xTris/glycine/SDS buffer for 15 min and then incubated with 2 x Laemmli sample buffer (BioRad) for 15 min ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x with 1X TBST for 15 minutes and developed using Clarity Western ECL Substrate (Bio-Rad, #1705060) and Molecular Imager Gel Doc (Bio Rad) ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515 ...
-
bioRxiv - Cell Biology 2022Quote: ... Purified GST-KIN-EC-ter fusion protein from pooled gel filtration fractions was cross-linked to Affi-gel 15 beads (BioRad) in MOPS ...
-
bioRxiv - Neuroscience 2023Quote: ... Heads (N = 15 for each genotype) were decapitated and homogenized in 100 µl 2X Laemmli Sample Buffer (BIO-RAD 1610737) containing 5% of 2-Mercaptoethanol (Sigma-Aldrich M3148) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... at 100 C and spun down on a microcentrifuge (eppendorf Centrifuge 5414) for 10 seconds prior to being loaded into 15-well 1mm 10% gels (BIORAD) with a total volume of 10-15 uL per lane and a total protein level of 15ug per lane ...
-
bioRxiv - Cell Biology 2023Quote: ... in pGEX-4T3 vector) was expressed and purified as described (Benz et al., 2008) and covalently coupled to Affigel 15 (Biorad). 800 ng in vitro phosphorylated or non-phoshorylated VASP was pulled-down with 5 µg GST-Zyxin in a buffer containing 40 mM Hepes-NaOH pH 7.4 ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated monomer was buffer exchanged and concentrated using 10 kDa MWCO Amicon Ultra-15 centrifugal filters prior to purification using the Superdex 200 HR 10/300 NGC Chromatography System (BioRad). The purified monomer was concentrated and stored at 0.5 mg/mL with 2 mg/mL leupeptin ...
-
bioRxiv - Neuroscience 2024Quote: ... onto 4-15% precast criterion sodium dodecyl sulfate polyacrylamide gel electrophoresis gradient gels and transferred to PVDF per manufacturer’s protocol (Bio-Rad). The protein sizing ladder used was Precision Plus Protein Standards (Bio-Rad) ...
-
bioRxiv - Biochemistry 2024Quote: ... and nsp9 were raised in rabbits (Cocalico Biologicals) and were affinity-purified against antigen covalently immobilized on beads (Affi-Gel 10 or Affi-Gel 15, BioRad). Rabbit polyclonal antibodies against mCherry [27] and the mouse monoclonal antibody against human protein C (HPC ...
-
bioRxiv - Microbiology 2024Quote: ... with transfer buffer (14.4 g/l glycine, 3 g/l Tris base and 15 % methanol) using mini-wet electroblotting system (Bio-Rad) at 55 V for 130 min ...