Labshake search
Citations for Bio-Rad :
1701 - 1746 of 1746 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Cell aliquots were thawed on ice for 5 minutes before adding 100 ng of plasmid DNA and electroporated on Bio-rad Micropulser (Bio-Rad, Hercules, CA) using EC2 protocol (0.2 cm cuvette ...
-
bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: ... were incubated together at RT for 5 min in the presence and absence of 5 mM 2-OG prior to loading on the analytical ENrich 650 column (BioRad Laboratories, Inc., Hecules, USA). The column was equilibrated with 50 mM Tris/HCl and 150 mM NaCl and supplemented with 5 mM 2-oxoglutarate when the complexes were preincubated in the presence of 5 mM 2-oxoglutarate and the applied proteins isocratic eluted with the respective buffer with a flow rate of 1 ml min-1 ...
-
bioRxiv - Neuroscience 2019Quote: ... qRT-PCR was carried out using iQ™ SYBR® Green Supermix on an iQ™5 multicolor real-time PCR detection system (Bio-Rad). Expression of PCR transcripts was calculated using the 2-deltaCt with Yhwaz serving as the internal control gene ...
-
bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
-
bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were performed in triplicate in a total volume of 20 μL that included 2 μL of the template or standard plasmid and 250 nM of each primer and TaqMan (5’ hydrolysis) probe (the RT-qPCR reactions also included 0.5 μL of Bio-Rad iScript advanced reverse transcriptase). Thermocycling conditions for qPCR consisted of an initial 50 °C step for 2 min followed by 95 °C for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
-
bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed three times for 5 min with 1x TBST and incubated with either an anti-mouse IgG- HRP-conjugate (1:5000, Bio-Rad Cat#172-1011) or an anti-rabbit IgG-HRP-conjugate (1:5000 ...
-
bioRxiv - Cell Biology 2021Quote: The supernatants of cell patches were collected after culturing under normoxic or hypoxic (5% O2) conditions and analysed using a fluorescence-dyed microsphere-based immune assay (Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
-
bioRxiv - Immunology 2022Quote: ... diluted to 1:5,000 in 1X TBST 5% milk powder for 1 hour before visualisation with Clarity™ Western ECL Substrate (Bio-Rad, Hercules, California, US) using a Syngene G ...
-
bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
-
bioRxiv - Microbiology 2020Quote: ... as per the manufacturer’s running conditions then activated for 5 min under UV to determine total protein (Bio-Rad ChemiDoc XR, Image Lab Software). Proteins were transferred to PVDF membranes (Bio-Rad) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times with 1X PBS for 5 min and incubated with goat anti-rabbit IgG-alkaline phosphatase-conjugate secondary antibody (Bio-Rad, 1:3000 dilution) for 1 h at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... washed in TBST and incubated with a recombinant human monoclonal anti-PAR antibody (1:2,500 in 5% nonfat dry milk-TBST; clones #19 and #21, highly specific for target-bound PAR, custom-designed at Bio-Rad AbD Serotec GmbH) (39 ...
-
bioRxiv - Cell Biology 2022Quote: ... the mixes were boiled at 97°C for 5 min and loaded in a Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hércules, EE.UU) at 12% acrylamide/bis-acrylamide ...
-
bioRxiv - Physiology 2022Quote: ... such that a total of 2 μg protein in 20 μL of buffer was loaded into wells of a Mini-PROTEAN TGX Stain-Free gel (5-14%) (Bio-Rad, Mississauga, ON, Canada). Protein ladder (5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
-
bioRxiv - Neuroscience 2023Quote: ... Gene expression for the 5 marker genes was quantified by qPCR using SYBR Green PCR Master Mix (Bio-Rad Laboratories Inc., Hercules, CA, USA), in three independent samples per treatment ...
-
bioRxiv - Cell Biology 2023Quote: ... They were then washed thrice with TBST (5 minutes/wash) at room temperature and signal developed for one minute using Clarity™ Western ECL substrate (Bio-Rad, Cat #: 1705060) or Clarity Max Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were then washed thrice with TBST (5 minutes/wash) at room temperature and then incubated with HRP-conjugated goat anti-rabbit IgG (H + L) (Bio-Rad, Cat #: 170-6515) or goat anti-mouse IgG (H + L ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were boiled at 97°C for 5 min and run on a 12% Mini-PROTEAN® TGX™ Precast Gel (Biorad, Hercules, USA) for 1 hour at 150V ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
-
bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
-
bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...
-
bioRxiv - Molecular Biology 2019Quote: ... RLUC was detected with a primary anti-RLUC (MBL Life Sciences PM047) 1:2000 in TBST 5% BSA and a secondary anti-Rabbit IgG-HRP (Bio-Rad 170-6515; 1:10000) antibody in TBST 5% milk ...
-
bioRxiv - Cell Biology 2021Quote: ... A 5-μL aliquot of the pre-amplified reaction was analyzed by qPCR using SsoFast EvaGreen Supermix (Bio-Rad Life Science, Inc., Hercules, CA, USA) and an Applied Biosystems 7900HT qPCR instrument (ThermoFisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... exposed to enhanced chemiluminescence plus west pico for 5 min and autoradiographed in a BioRad Gel-Doc System (Bio-Rad Laboratories Inc., Hercules, CA, USA). The loading consistency was verified utilizing the house-keeping proteins (Rab11 and β-Actin ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were dissolved in 25 μL of double distilled water (boiled at 95 °C for 5 minutes) containing loading buffer (4X; Bio-Rad Laboratories, Mississauga, ON, Canada) and reducing agent (20X ...
-
bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out by mixing 45 µl of competent cells with 100-200 ng of plasmid and subjecting the cells to a short 5 ms electric pulse under a 12500 V/cm electric field in a Gene Pulser II® (Bio-Rad, Hercules, CA, USA) (2500 v ...
-
bioRxiv - Biophysics 2024Quote: ... the solution was heated and slowly cooled from 80°C to 4°C (ramp rate of -1°C per 5 s) in a standard thermocycler (Bio-Rad CFX96 Real-Time System) prior to each experiment.
-
bioRxiv - Neuroscience 2021Quote: ... Samples were clarified by a brief centrifugation (10,000 g for 5 min at 4°C) and the supernatants were collected and diluted with 2× reducing Laemmeli buffer (Bio-Rad, Hercules, CA, USA, cat. #1610737). For preparation of cell culture lysates ...
-
bioRxiv - Plant Biology 2021Quote: GV3101 electro-competent Agrobacterium tumefaciens strain was incubated on ice with ~100 ng plasmid for 5 min and electroporated using a MicroPulser (Bio-Rad Laboratories; 2.2 kV, 5.9 ms). Immediately after electroporation ...
-
bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
-
bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed using iQ™ SYBR®Green Supermix on an iQ™5 multicolor real-time PCR detection system (Bio-Rad, CA, USA). All primers were obtained from Sigma-Aldrich and described in Suppl ...
-
bioRxiv - Plant Biology 2024Quote: ... Six μL cDNA (diluted 1:5 with distilled water) was used per qRT-PCR reaction in a 15-μL reaction volume using SYBR Green (BIO-RAD, iTaq Universal SYBR Green Supermix) and a BIO-RAD CFX96 real time PCR instrument ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
-
bioRxiv - Biochemistry 2021Quote: ... All final samples were boiled in 30 µL NuPAGE LDS sample buffer and sample reducing agent for 5 min and loaded on Mini-PROTEAN® TGX Stain-Free™ Gels (Bio-Rad laboratories, Hercules, CA, USA). Electrophoresis was performed at 300 V for 3 min using the BIO-RAD Mini-PROTEAN® Tetra System ...
-
bioRxiv - Microbiology 2023Quote: ... Blocking of the membrane was carried out by incubating in blocking solution for 1 h at room temperature (5% Bio-Rad Blotting-Grade Blocker in TSB-T buffer). Membrane was then incubated with anti-PcdA polyclonal antibody diluted 1:15,000 in blocking solution for 2 h at room temperature.