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Citations for Bio-Rad :
1701 - 1750 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1:400) to identify αβT cells and mouse anti-pig SWC5 (clone b37c10, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of γδT cell subpopulation ...
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bioRxiv - Biochemistry 2020Quote: ... and pellet dissolved in the original sample volume of 1:1 PBS: SDS loading buffer were then analyzed by SDS-PAGE (Bio-Rad) at 160 V for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions) in a ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: Proteins were reduced with 1 mM TCEP for 1 h then passed through a 10DG desalting column (Bio-Rad, Hercules, CA) to remove the TCEP ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk and incubated with anti-GFP (1:1000) followed by Amersham ECL goat anti-rabbit horseradish peroxidase conjugate 1:5000 (Bio-Rad). Blots were developed using chemiluminescence (Super Signal West Femto ...
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bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... was added to each well and the mixture was heated from 25 to 95°C in 1°C steps of 1 min each in a CFX96 Touch™ Real-Time PCR Detection System (BioRad). Excitation and emission filters of 492 and 516 nm respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) or goat anti-mouse IgG-HRP conjugate (1:3000) (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... pre-run for 1 hour at 4 ° C and run for 1 hour at 120 volts on Mini-Protean 3 gels (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... This step was repeated for 3 times and the samples were concentration-matched at OD500nm = 10 and mixed 1:1 with 2x Laemmli buffer (Bio-Rad), containing SDS and 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... at 1-4 °C and 100 volts for 1 h using Criterion™ blotter with wire electrodes (BioRad, Cat#1704071, USA). Blots were rinsed with phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2024Quote: ... for 1 hour RT followed by 1:2000 IRDye800 conjugated goat-anti-rabbit antibody (Li-COR Biosciences) or 1:1000 goat-anti-rabbit HRPO (Bio-Rad) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... and blocked in Odyssey PBS blocking buffer 1:3 in 1x PBS (Li-Cor, 927-40000) or EveryBlot blocking buffer 1:3 in 1x PBS (Biorad, 12010020) for 30 min (Odyssey ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were then exposed to HRP-conjugated secondary antibody for 1 hour (1:10 000 Goat anti-rabbit IgG HRP conjugate; Bio-Rad). Antibody-protein complexes were visualized using enhanced chemiluminescence detection reagents (Clarity Western ECL Substrate ...
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bioRxiv - Cancer Biology 2024Quote: Pellets of 1×106 treated cells were incorporated in 1% agarose at 50°C and cast into molds (Biorad, Grabels, France) avoiding air bubbles ...
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bioRxiv - Plant Biology 2024Quote: ... IgG (H+L)-HRP (1:3000) and goat anti-rabbit IgG (H+L)- HRP (1:3000) conjugated secondary antibodies (Bio-Rad) were used.
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription reactions were performed using 4 µL of iScript at room temperature with 1 µg-1 pg of RNA template (Bio-Rad) in a final volume of 20 μL ...
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bioRxiv - Genomics 2024Quote: ... cDNA was diluted 1:1 with dH2O and prepared in a 20ul volume (10ul of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), 8ul of dH2O ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP-1 (BMK-13, MCA5751 Bio-Rad) and rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated on ice for 15 minutes in flow cytometry buffer (PBS, 1% BSA, 0.25% NaN3) containing 1% Mouse SeroBlock FcR (Bio-rad, BUF041A). A 647-conjugated anti-TREM2 antibody (R&D ...
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bioRxiv - Plant Biology 2023Quote: ... and CCD1 (5’-GGGAAGAGGGTGATGAAGTTGT-3’ and 5’-TGATATCCATTCACCTTGTCCAAA-3’) and 5 µl of SsoAdvanced Universal SYBR Green Supermix (172-5270; BioRad, Hercules, CA, USA). All samples were analyzed in triplicate using the CFX connect Real-Time PCR Detection System (1855201 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Genomics 2021Quote: ... at 4°C in 1×TAE buffer (40 mM Tris-acetate, 1 mM EDTA) using 0.55% Certified Megabase agarose gel (Bio-Rad, 161-3109). Short-range PFGE ran at 4.2 or 4.5 V/cm with a pulse time from 40 to 70 s for 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated for 1 h at RT with goat anti-Lipid A LPS antibodies (diluted 1:1,000 in blocking buffer; Bio-Rad; Cat # OBT1844). Blots were washed three times with TBST (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed for 1 hour at room temperature with 1:500 anti-XLF(New England Peptides, details in Methods under Immunodepletion) or 1:1000 anti-His (Bio-Rad MCA1396A) in PBST with 2.5% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% dry non-fat milk in PBST and probed using PRP8a antibody (1:500) and an HRP coupled anti-Rabbit secondary antibody (Biorad, 1:10000). All antibodies were diluted in 1% dry nonfat milk in PBST ...
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bioRxiv - Bioengineering 2020Quote: ... and annealed from 90°C to 20°C at 1 °C min-1 in a T100™ Thermal Cycler (Bio-Rad, USA). Following construction ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (28) ...
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bioRxiv - Biochemistry 2023Quote: ... different amounts of TTR1st in the range 0.1 1−1.10 μg were loaded in a native 4-15% PAGE gel (Mini-PROTEAN® TGX™ Precast Gel, Bio-Rad). The gel contained colored molecular weight standards (Precision Plus Protein Dual Color Standards ...
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bioRxiv - Immunology 2023Quote: ... The samples and a protein ladder (a 1:1 mix of All Blue Prestained Protein Standards: Unstained Protein Standards; Precision Plus Protein™, Bio-Rad) were loaded to 4-15% mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... A 1:1 Langmuir binding model was used to fit the data measured on a ProteOn™ XPR36 instrument (Bio-Rad Laboratories) using ProteOn™ Manager Software (Version 3.1.0.6 ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Neuroscience 2024Quote: ... The immunoprecipitated protein was solubilized with the elution buffer (1:1 mixture of NET buffer with 4X Laemmli buffer (Bio-Rad #1610747), and 10% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated with primary antibodies 1:1000 rabbit anti-Iba1 (FujiFilm, 019-19741) and 1:300 rat anti-Cd68 (Bio-Rad, MCA1975) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... molten 1% certified low melt agarose (Bio-Rad, 1613112) in PBS was equilibrated to 45°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by instillation of 1% low melting agarose (BioRad) through the trachea to prevent leakage of dispase ...
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bioRxiv - Cell Biology 2020Quote: ... Sheep anti-TGN46 from Biorad (AHP500, Immunofluorescence 1:500), Hoechst 33342 from Invitrogen Molecular Probes (H3570).
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bioRxiv - Immunology 2021Quote: ... CD68 (1:200; Bio-Rad, cat no. MCA1957, monoclonal), and C1q (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-AMH (1:20, MCA2246, Bio-Rad Laboratories), rabbit anti-PAX8 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Rabbit α-Mouse-HRP (Bio-Rad, STAR13B, 1:2000), and Rec-G-Protein-HRP (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and CD11b (1:500, #MCA275R, Bio-Rad, CA, USA). Samples were washed with 1X PBS and incubated with Alexa fluorophore-conjugated secondary antibodies (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and StarBright Blue 700 anti-rabbit (1:5.000, BioRad) in TBS-T containing 5% milk powder ...
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bioRxiv - Molecular Biology 2020Quote: ... rat polyclonal anti-α-tubulin (1:5000, Bio-rad), mouse monoclonal anti-Flag M2 (1:2000 ...
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bioRxiv - Genomics 2022Quote: ... gel was prepared in 1× TAE buffer (BIO-RAD). DNA samples were loaded together with Quick-Load 1 kb Extend DNA Ladder (NEB ...