Labshake search
Citations for Bio-Rad :
1601 - 1650 of 5665 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a PVDF membrane using a Mini Trans-Blot® Cell (Bio-Rad Laboratories) at 100V for 2h ...
-
bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transfer to a PVDF membrane using Trans-Blot Turbo Transfer system (Bio-Rad), blocked in 3% milk PBST and incubated overnight at 4°C with primary antibodies ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The proteins were electro-transferred into nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and blocked overnight at 4°C in blocking buffer (5% milk powder (w/v) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The samples were transferred onto a Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Pack (BIO-RAD) and probed using Anti-YmoA or anti-Yops/YopE polyclonal antibodies ...
-
bioRxiv - Neuroscience 2024Quote: ... The separated proteins were transferred onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). To block nonspecific binding ...
-
bioRxiv - Cell Biology 2024Quote: ... in MOPS running buffer and transferred to nitrocellulose membranes using the Mini Trans-Blot Cell system (Biorad). The membrane was blocked with 10% milk in Tris-buffered saline-Tween (TBS-T ...
-
bioRxiv - Biochemistry 2024Quote: ... Afterwards proteins were transferred onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (BioRad, 1704270, 1704159). Nitrocellulose membranes were blocked at RT in blocking solution containing 5% milk powder in TBST (20mM Tris pH 7.5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-rad; Hercules, CA). Membranes were incubated overnight in primary antibody ...
-
bioRxiv - Cell Biology 2024Quote: ... The separated proteins were transferred to a nitrocellulose membrane using a Mini Trans-blot Module (Bio-Rad) at constant 100V for 60-90 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad, Cat#1704158/1704159) and membranes blocked with 5% non-fat milk for 1 h ...
-
bioRxiv - Cell Biology 2024Quote: ... TGX gels were activated via UV light application using the Trans-Blot Turbo transfer system (Bio-Rad). While blotting ...
-
bioRxiv - Biophysics 2023Quote: ... Protein was transferred to a PDVF membrane using Trans-Blot Turbo Transfer System (Bio-Rad; Hercules, CA) at 7 V ...
-
bioRxiv - Microbiology 2022Quote: ... Wako Chemicals) in Tris-MOPS running buffer (Wako Chemicals guidebook) for 2h at 100V using a Mini-PROTEAN® Tetra system (Bio-Rad Laboratories). After migration ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein extract (4 ug per lane) was separated on a 10% TGX Stain-Free polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Systems Biology 2022Quote: Cell extracts (15 ug) were resolved by SDS-PAGE using 4-15% TGX precast gels (Bio-Rad #4561086) with tris/glycine/SDS buffer and transferred onto PVDF membranes using the Bio-Rad Mini-PROTEAN Electrophoresis System ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 50 ug of protein was loaded onto 4-15% Mini-Protean TGX (4561084, Bio-Rad, Hercules, CA) gels ...
-
bioRxiv - Microbiology 2020Quote: ... and cDNA was synthesized from 0.1 ug of aphid RNA using the iScript cDNA Synthesis Kit (Bio-Rad). PLRV titer in undiluted cDNA was quantified by digital drop PCR using EvaGreen Supermix and the QX100 droplet digital PCR system as previously described (51).
-
bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
-
bioRxiv - Genetics 2023Quote: ... 8.5 ug of each protein sample was loaded onto a 4–20% Stain-free Protein Gel (Biorad #5678093) with a dual-fluorescent ladder (Biorad #1610374) ...
-
bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
-
bioRxiv - Biophysics 2022Quote: ... Nanodisc reconstitution was induced by removing detergent with 0.8 mg ml-1 pre-washed Biobeads SM-2 (Bio-Rad) for 2 hours with gentle agitation at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the appropriate resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively with buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates prepared at a concentration of 1 mg/ml and supplemented with 4x Laemmli Sample Buffer (Bio-Rad: 1610747) supplemented with fresh 2-mercaptoethanol (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the Amylose resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively ...
-
bioRxiv - Microbiology 2022Quote: ... A 15% SDS-Tricine gel was made by mixing: 2.5 mL 30% acrylamide/Bis solution 37.5:1 (BioRad #1610158), 1.25 mL gel buffer (1.5 M Tris-HCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
-
bioRxiv - Genetics 2023Quote: ... followed by purification and size selection on 1% agarose gel with .6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
-
bioRxiv - Microbiology 2020Quote: ... lipid vesicles (100 μg/ml) were immobilized to a neutravidin-coated NLC chip (Bio-Rad, USA) at a flow rate of 30 μl/min to a coupling level of about 1000 resonance units (RU) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 0.17 mg/ml Zymolyase 100 T (Nacalai) and poured into a plug mold (Bio-Rad). Agarose was allowed to solidify at 4°C for >30 min ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...
-
bioRxiv - Microbiology 2019Quote: ... The membrane was washed three times with T-TBS for 3 x 5 min and incubated for 1 h with 1/1000 solution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (1:1000; Biorad) and washed 3 times with T-TBS and 1 time with TBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
-
bioRxiv - Immunology 2019Quote: Mouse patellofemoral joints and hearts were homogenized using an electric homogenizer with toothed blades in tissue lysis buffer (Machery-Nagel, 740962) containing beta-mercaptoethanol (1:100) (Bio-Rad, 161-0710) and Triton-X-100 (1:100 ...
-
bioRxiv - Cell Biology 2019Quote: ... cells from a 10 cm dish were pelleted and lysed with 30 µl lysis buffer (20 nM Tris-HCl, 150 mM NaCl, 1% Triton X-100 (Bio-Rad, Hercules, CA), 20 mM beta-glycerol phosphate and protease inhibitors cocktail ...
-
ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to 1:1 by adding appropriate amount of ECL (Bio-Rad) color solution in the dark conditions and photographed using an EZ-ECL chemiluminometer Detection Kit for HRP (Biological Industries ...
-
bioRxiv - Cell Biology 2022Quote: ... Supernatant was diluted 1:1 with 2X Laemmli sample buffer (BioRad, 1610737) under reducing conditions and boiled for 5 minutes ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1:1000 mouse anti-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360), or 1:10,000 mouse anti-actin (C4 ...
-
bioRxiv - Neuroscience 2019Quote: ... Protein sample were diluted 1:1 with 2X SDS Laemmli buffer (Biorad) and incubated for 30 mins at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: ... estrogen receptor (Santa Cruz sc-542, 1:20; BioRad MCA1799T, 1:25), Ltf (Bioss bs-5810 ...
-
bioRxiv - Physiology 2021Quote: ... Samples were mixed 1:1 with Laemmli’s sample buffer (BioRad 161-0737) containing 10% 2-Mercaptoethanol (Sigma M3148 ...
-
bioRxiv - Immunology 2022Quote: ... samples were mixed 1:1 with 2 x native sample buffer (BioRad) and loaded on a 4-20% precast protein gel (BioRad ...
-
bioRxiv - Cell Biology 2024Quote: ... Rat anti-F4/80 (Bio-Rad, clone CI:A3-1, #MCA497GA, 1:50), Rabbit anti-CD45 (Abcam ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein purity was analyzed using 12% stain-free TGX gels detected via the ChemiDoc MP Imaging System (BioRad). The protein was dialyzed against “storage buffer” (10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein purity was evaluated by SDS/PAGE in a 4-20% precast polyacrylamide gradient gel (BioRad, Copenhagen, Denmark). Protein concentrations were estimated according to Bradford ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein purity was analyzed via SDS-PAGE and protein concentration was measured using Bradford (Bio-Rad protein assay). GST-Rap2a Lysine mutants (K2R ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant protein purities were assessed by SDS-PAGE using 4-20% Mini-Protean TGX gels (Bio-Rad, #4561094) and quantified using the BCA Protein Estimation kit (ThermoFisher Scientific ...