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Citations for Bio-Rad :
1601 - 1650 of 2612 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... After staining with R-250 Coomassie Blue (Biorad), proteins were digested in-gel using trypsin (modified ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR (qPCR) was performed with primer sets corresponding to primer list (Table 3) and using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 172-5125) on an Applied Biosystems 7500 Fast Real-Time PCR system or ThermoFisher Scientific QuantStudio 3 or 5 Real-Time PCR system ...
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bioRxiv - Biochemistry 2022Quote: ... The plate was centrifuged at 2000rpm (3 minutes at room temperature) and placed in the CFX Touch™ Real Time PCR Detection System (Bio-Rad; Hercules, California, USA) set for an initial denaturation (95°C ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Then, 3 mL of each culture were pelleted (11,000 rpm, 1 min) to perform total RNA extraction (using BioRad’s Aurum™ Total RNA Mini Kit). RNA concentration and purity were assessed using a nanodrop and all samples shown to have A260/A280 and A260/A230 ratios ≥ 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were denatured and proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on Mini-Protean 10% tris-glycine gels (BioRad). Precision plus protein Kaleidoscope molecular weight (MW ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were prepared for mass spectrometry by disrupting total or immunopurified ribosomes in 1X Laemmli buffer and running on a 4-20% Mini-PROTEAN TGX Precast Protein Gel in 1X Tris/Glycine/SDS buffer (BioRad) for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Physiology 2022Quote: ... were run on ice at 90 V for 30 min and then at 120 V until the dye front migrated to the opposite edge of the gel on ice in 1X Tris / Glycine / SDS electrophoresis buffer (BioRad). Samples were wet-transferred to nitrocellulose (0.2 µm ...
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bioRxiv - Biochemistry 2021Quote: ... 15 μg of proteins were loaded on a 10% Tris–glycine precast SDS-PAGE gel (Mini-PRO-TEAN TGX, BioRad). After a short migration (0.5 cm ...
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bioRxiv - Bioengineering 2021Quote: Protein samples were mixed with Laemmli buffer and the proteins were separated by SDS-PAGE using either a 7.5 % or a 12 % polyacrylamide gel and a Tris-glycine running buffer (Bio-Rad). The Precision Plus Protein™ Unstained Standard (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then transferred onto low-fluorescence 0.45 μm PVDF membranes with transfer buffer (10× Tris/glycine with 10% methanol) (Bio-Rad) for 10 min using the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad). Proteins were then transferred onto low-fluorescence 0.45 μm PVDF membranes with transfer buffer (10× Tris/glycine with 10% methanol ...
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bioRxiv - Molecular Biology 2020Quote: ... Running buffer was prepared with tris-glycine 1X and SDS 0.1% using a homemade gel (7-12% bis-acrylamide, Bio-Rad), which ran at 100 V for approximately 2:30 h ...
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bioRxiv - Cell Biology 2021Quote: ... 20-30 μg of total proteins from each sample were separated by SDS/polyacrylamide gel electrophoresis in running buffer (25 mM Tris pH 8.3, 192 mM Glycine, 0.1% SDS) and transferred onto nitrocellulose (NC) membranes (Bio-Rad, 1620094) in transfer buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein sample was incubated at 98°C for 5 min and ran on Bio-Rad TGX Stain-Free Precast Gel (4-15%) in Tris/Glycine/SDS running buffer (BioRad). Then the gel was transferred onto a 0.45 μm PVDF membrane (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: ... The whole cell lysate and crude cytoskeletons were resolved on 4-20% Mini-PROTEAN tris-glycine polyacrylamide gels (Bio-Rad), and Western blots were stained with 1:5000 diluted anti-beta-actin mouse monoclonal antibody (Cell Signaling Technology ...
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bioRxiv - Physiology 2022Quote: ... Total protein (30 μg) per lane was separated by SDS-PAGE using 4–15% Tris-glycine gels (Bio-Rad Laboratories) and transferred to PVDF membrane (Millipore Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were normalized for protein concentration and subjected to SDS-PAGE separation on stain-free tris-glycine gels (Bio-Rad), cross-linked and imaged with the Chemidoc system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Gels were run at room temperature for 90 minutes at 90 volts in 1X Tris/Glycine/SDS running buffer (BioRad). Blots were transferred to nitrocellulose membranes using the Mini Trans-Blot® system (BioRad ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... 40 μg of cell lysates was separated by 4-20% Tris-glycine MINI-PROTEAN TGX gels (Bio-Rad, 456-1094) and transferred to PVD membranes using Trans-Blot Turbo Transfer Pack (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared in Licor protein sample loading buffer and run on TGX 7.5% and 4-20% Tris-glycine gels (Bio-Rad) and blotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2023Quote: ... Samples containing 20 µg of protein were separated on 4-20% Tris-Glycine SDS-PAGE gel (Bio-Rad, CA, USA) and transferred to PVDF membrane using Trans-Blot Turbo transfer system and transfer packs (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... we transferred the material to a PVDF blotting membrane using a transfer buffer (10x Tris/Glycine Buffer for Western Blots, BioRad). We performed a wet transfer at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... were loaded on a 12% SDS-PAGE gel and run for 1h at 150V in Tris-glycine SDS buffer and transferred onto a nitrocellulose membrane using semi-dry transfer (Transblot turbo, Biorad). Immunoblot detection was performed using antibodies raised against FlvB (1:1,000)(Chaux et al. ...
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bioRxiv - Microbiology 2024Quote: ... and the cleared lysate was loaded onto 4-20% Tris-glycine gels (MINI-Protean TGX Pre-cast, Bio-Rad, 4561095) and electrophoresed at 200 V for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and heat denatured at 95°C for 10 min before loading onto a 4-15% Tris-glycine gel (Bio-Rad) and electrophoresed at 125 V ...
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bioRxiv - Neuroscience 2024Quote: ... The adapted protein quantity per lane was loaded into 4-20% 1.5 mm Tris-glycine 15 well mini gels (Bio-Rad) with 1X Laemmli sample buffer ...
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bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
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bioRxiv - Plant Biology 2024Quote: ... Ten microliters of isolated total protein were separated on a 4-20% Tris-glycine stain free polyacrylamide gel (Bio-Rad) at 180 V for one hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and 15 µg of each sample was loaded into a Tris-Glycine 4-20% Stain-Free polyacrylamide gel (Bio-Rad). After electrophoresis ...
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bioRxiv - Genetics 2024Quote: ... Samples were transferred from the Tris-Glycine gel to the PVDF membrane using a Mini Trans-Blot Electrophoretic Transfer Cell (Biorad) at 0.03A for 18-20 hours ...
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bioRxiv - Biochemistry 2024Quote: ... 20ug of input cell lysates and 10ul of immunoprecipitated elutes were run on 4-20% Tris glycine gradient gels (Biorad). For detecting protein biotinylation ...
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bioRxiv - Immunology 2023Quote: ... samples were run on either 4 to 15% or 4 to 20% Mini-PROTEAN TGX™ Tris-Glycine gels (BioRad) for 40-60 min at 160 V ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were sonicated prior to being separated by SDS PAGE using a 4-20% Tris-glycine polyacrylamide pre-cast gel (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2022Quote: ... The same quantity of samples used for the competition assay was run on a 4%–20% gradient Tris-Glycine Gel (BioRad) and transferred to nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were boiled with SDS-PAGE sample loading buffer (6X SDS) and 30 ug of protein was loaded to each well of a 10% Tris-glycine SDS-PAGE gel (BioRad). Proteins were transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... 20% DMSO and 0.3M Glycine for 1h followed by a blocking step using an aqueous solution of 0.2% Triton X100 (Bio-Rad Laboratories), 0.2M Tris pH8 ...
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bioRxiv - Microbiology 2022Quote: ... and 15 μl of each was loaded on to a 4 - 15% polyacrylamide Tris/Glycine minigel (Bio-Rad, Hercules, CA) and separated at 100 V ...
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bioRxiv - Immunology 2022Quote: ... before being resolved by tris-glycine SDS-PAGE and transferred onto nitrocellulose or PVDF membranes at 25V using a semidry Trans-Blot Turbo (BioRad). Membranes were blocked for 1 hour with milk (5% w/v ...
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bioRxiv - Molecular Biology 2023Quote: Equivalent amounts of 5-15 μg of protein for gel loading (cell lysates, mitochondrial/cytoplasmic fractions) were separated by SDS/PAGE on 4-20% Tris/glycine precast gels (BioRad) and transferred to PVDF membranes (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... and SDS-PAGE was performed on 20 µg of cell lysate per sample on Tris-glycine-SDS polyacrylamide gels (4561095, Biorad) then transferred to PVDF (1620177 ...
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bioRxiv - Immunology 2023Quote: ... SDS-PAGE was performed at 100 V on 4-15% gradient acrylamide Stain-free gels in Tris-Glycine-SDS buffer (BioRad), with 5-10 µg of protein ...
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bioRxiv - Neuroscience 2023Quote: ... 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel, #567-1085, Bio-Rad) under denaturing conditions ...
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bioRxiv - Microbiology 2024Quote: ... Protein lysates were resolved on SDS-PAGE in a 4-15% TRIS-glycine gel (BIO-RAD Mini -PROTEAN® TGX )TM together with a molecular weight marker ...
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bioRxiv - Neuroscience 2024Quote: ... which was run in 1x running buffer diluted from a 10x Tris/Glycine/SDS stock (cat. # 1610772; Bio-Rad Laboratories) at 160 V for 40 min ...