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Citations for Bio-Rad :
1601 - 1650 of 2130 citations for IL 2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse secondary antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and anti-mouse secondary antibody (Biorad; 1:10,000). GroEL was detected with anti-GroEL primary antibody (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... culture supernatants were collected and cytokine levels in the samples were measured using a Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used the multiplex bead array system Bio-Plex Pro Mouse Chemokine Assay Panel 31-Plex (#12009159, Bio-Rad Laboratories GmbH), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from mouse tissues using the Aurum Total RNA Fatty and Fibrous Tissue Extraction kit (Biorad: #732-6870). RNA from cells was extracted using Zymo Research R1055 Quick-RNA MiniPrep Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were washed three times in TBST for 5 minutes each and incubated with an anti-mouse HRP secondary (Bio-Rad) at 1:2000 dilution for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed thrice and incubated with a secondary antibody (Goat anti-Mouse IgG-HRP, 1706516, Bio-Rad Laboratories, 1:10K), also diluted in 2.5% milk in TBST ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibody (Goat Anti-Mouse Starbright Blue 520 and Goat Anti-Rabbit Starbright Blue 700, each at 1:2500, Bio-Rad). Blots were analyzed (ChemiDocTM MP Imaging System ...
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bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Bovine CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using a mouse anti-bovine CD4 antibody (clone CC8, Bio-Rad), anti-mouse IgG MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Genetics 2023Quote: ... primary antibodies – anti-TY1 and rabbit anti-HA (GTX115044) – and fluorescent secondary antibodies – anti-mouse Starbright Blue 520 (Bio-Rad 12005867) and anti-rabbit Starbright Blue 700 (Bio-Rad 12004162 ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) or goat anti-mouse IgG-HRP conjugate (1:3000) (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... were used; and as secondary antibody HRP-conjugated anti-rabbit or anti-mouse (both 1:10 000; anti-rabbit (1706515; Bio-Rad); anti-mouse (170-6516 ...
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bioRxiv - Plant Biology 2023Quote: ... anti-rabbit IgG (H + L) secondary antibody and goat peroxidase-conjugated anti-mouse IgG (H + L) secondary antibody were purchased from Bio-Rad.
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Immunology 2023Quote: ... plates were washed thrice before addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (170-6516; Bio-rad, Hercules, California, USA), anti-mouse IgG1 (ab97240 ...
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bioRxiv - Cell Biology 2023Quote: ... Peroxidase-conjugated goat anti-mouse (170-6516) and peroxidase-conjugated goat anti-rabbit (1706515) secondary antibodies were purchased from Bio-Rad.
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bioRxiv - Bioengineering 2023Quote: ... followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000, 1721011, Bio-Rad, RRID: AB_11125936). Detection of the horseradish peroxidase signal was carried out using Clarity Western enhanced chemiluminescence (ECL ...
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bioRxiv - Physiology 2023Quote: ... The secondary antibodies were goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP conjugate (Bio-Rad, Hercules, CA, USA) for western blotting ...
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bioRxiv - Microbiology 2023Quote: ... mouse cDNA was analyzed in a 10-μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The following day after washes with TBS-T and incubation with secondary antibodies (Goat Anti-Rabbit or Goat Anti-Mouse IgG (BioRad 1706516) 1:5000 dilution in 10% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were pelleted and washed once with 0.75 ml of ice cold 0.5% TCA and resuspended in 2× Laemmli sample buffer (Bio-Rad Laboratories). Western blot was performed as described (Hsu et al. ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Neuroscience 2019Quote: ... samples were prepared on ice (to a final volume of 40 microliters) in a mixture of Laemmli Sample Buffer and 2-Mercaptoethanol (BioRad) and then vortexed and denatured for 10 min at 70°C ...
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Plant Biology 2019Quote: ... 2% of samples were used as templates to perform quantitative PCR (qPCR) using SYBR Green PCR Master Mix (Bio-Rad) with primers recognizing the Ds element or an unrelated intergenic region in soybean chromosome 7 ...
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bioRxiv - Developmental Biology 2019Quote: ... monomeric ATP-bound 2 μM rabbit muscle actin was treated overnight at 4°C with analytical grade anion exchange resin (BioRad), hexokinase (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... Peak fractions from SEC purification were pooled and 2-mercaptoethanol was removed with Econo-Pac 10DG desalting column (Bio-rad) which was pre-equilibrated with buffer C ...
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto 0.2 µm pore nitrocellulose membranes for 10 min at 2.5 V using the Trans-blot turbo transfer system (Bio-Rad). For more sensitive detection of neurexin levels ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... 95°C), and run (loaded in 2 wells, 20 μL per well) on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mg/ml Orange G), denatured (2 min, 80°C) and run on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Plates were imaged together with a 1 cm2 reference area on black background (see example in Supplementary Data 2) using a Gel-Doc XR + gel documentation system (BioRad). Images were analysed in MATLAB using the code in Supplementary Note 2 to determine the growth area (in cm2 ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...