Labshake search
Citations for Bio-Rad :
1601 - 1650 of 4878 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... 3 μg of total RNA was used in the reverse transcription reaction using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR program was run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (#1725121, Bio-Rad, Hercules, CA,) using human Il6 and mouse B2m ...
-
bioRxiv - Immunology 2020Quote: ... IL-6 and IL-17 with housekeeping gene β-actin mRNA by qPCR using the iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). Forward and reverse primers and PCR conditions for RT-PCR are listed in Table 1 ...
-
bioRxiv - Cell Biology 2019Quote: ... and the beads were washed with buffer #2 in Poly-Prep columns (Bio-Rad). Complexes were eluted in two fractions with buffer #2 supplemented with 2.5mM D-biotin ...
-
bioRxiv - Cell Biology 2020Quote: ... and sets of gene-specific forward and reverse primers (ABCA1, CCL-2) (Bio-Rad) and subjected to real-time PCR quantification using the CFX384 Real Time PCR ...
-
bioRxiv - Neuroscience 2021Quote: ... for 2 hours at room temperature and developed with ECL (Biorad 40-720-71KIT). Blots were quantified using FIJI software.
-
bioRxiv - Biochemistry 2021Quote: ... followed by detergent removal using 0.8 mg pre-washed Biobeads SM-2 (Bio-Rad) per ml of reaction mix for 2 hours at RT while slowly rolling ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse-transcribed (2 ug) into cDNA (iScript kit, BioRad Laboratories, Hercules, CA) and analyzed by qRT-PCR ...
-
bioRxiv - Biochemistry 2020Quote: ... The detergent/lipid/enzyme solution was mixed with SM-2 bio-beads (Bio-Rad) for 2 h at 4 0C to remove the detergent ...
-
bioRxiv - Biophysics 2021Quote: ... Detergent was removed with four rounds of incubation with SM-2 beads (Bio-Rad) at 80mg beads per 1 mL of liposome suspension (2 hrs at 23°C twice ...
-
bioRxiv - Biophysics 2020Quote: ... Triton X-100 was then removed by adding BioBeads SM-2 absorbent beads (BioRad) at a Bio-Beads/Triton X-100 ratio of 10 (wt/wt ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 to 15 µl of samples were dotted onto a nitrocellulose membrane (Bio-RAD). We used a concentration of 6E10 antibody of 1:2000 (Biolegend® ...
-
bioRxiv - Bioengineering 2022Quote: ... using the primers listed in Supplementary Table 2 using SsoFast EvaGreen Supermix (Bio-Rad). The expression of the respective gene of interest was normalized to the samples’ respective GAPDH expression using the ΔCt or ΔΔCt method as indicated in the figures ...
-
bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and loaded onto a 2 ml CHT2-I hydroxyapatite column (Bio-Rad) equilibrated in 20 mM HEPES ...
-
bioRxiv - Microbiology 2021Quote: ... cell lysates and immunoprecipitates on beads were resuspended in 2× Laemmli buffer (Bio-Rad) and subjected to SDS-PAGE and western blot analysis.
-
bioRxiv - Bioengineering 2021Quote: ... 2% milk solution was prepared by dissolving nonfat dry milk powder (Bio-rad, UK) in distilled water and this solution was mixed with equal volume of 2% ...
-
bioRxiv - Immunology 2022Quote: ... for 2 hours at 4 °C using Mini-trans blot wet tank transfer (BioRad). After transfer ...
-
bioRxiv - Neuroscience 2019Quote: ... Bound proteins were eluted in 2 × Laemmli sample buffer (Bio-Rad, Cat# 161-0737) containing 5% 2-mercaptoethanol by heating at 95 °C for 5 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... sections were blocked with 2% milk powder (blotting grade; Bio-Rad Laboratories, Hercules, USA) in PBS for 30 minutes ...
-
bioRxiv - Genomics 2021Quote: These reactions were undertaken on a DNA Engine Tetrad 2 Thermal Cycler (Bio-Rad), with MyTaq HS DNA polymerase ...
-
bioRxiv - Plant Biology 2022Quote: ... Extracted proteins were further purified using the BioRad ReadyPrep 2-D Cleanup Kit (BioRad), approximately 60-70 μg of protein was loaded onto 7 cm pH 3-10 NL and pH 4-7 IPG strips (BioRad ...
-
bioRxiv - Plant Biology 2022Quote: ... Luminescence intensities mm-2 were evaluated using the Image Lab software (BioRad, Feldkirchen, Germany).
-
bioRxiv - Microbiology 2022Quote: ... The cells:DNA mix was transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082) and electroporated at 2500 kV ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 2 h on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
-
bioRxiv - Immunology 2022Quote: ... and then blotted for 2 hours onto 0.45 μm supported nitrocellulose membranes (Bio-Rad). Membranes were blocked for one hour using 5% Bovine Serum Albumin (BSA ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated for 2 minutes with Clarity Western ECL Blotting Substrate (Bio-Rad) and proteins visualized on ChemiDoc (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... then 2 ul of normalized RNA were mixed with iTaq Universal SYBR (Bio-Rad) and primers for c-Myc (F ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46; Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: ... and re-suspended in 2x Laemmli sample buffer containing 2% SDS (Biorad, catalog #: 1610737). Protein concentration was measured by MicroBCA assay (Thermo Scientific ...
-
bioRxiv - Biophysics 2023Quote: ... Afterwards the samples were incubated with Bio-Beads SM-2 sorbent (Bio-Rad Laboratories) overnight on the rolling bank at 4°C to remove the detergent (Rigaud et al ...
-
bioRxiv - Biochemistry 2023Quote: ... detergent was removed by overnight incubation with activated Bio-Beads SM-2 (Bio-Rad). For purification of PhuR-containing nanodiscs ...
-
bioRxiv - Plant Biology 2023Quote: ... Each reaction contained 5 μL of 2 x iQ SYBR Green supermix (Bio-Rad), 2 μL of diluted cDNA (10 times dilution for shoots and roots ...
-
bioRxiv - Microbiology 2024Quote: ... the beads and total lysate were resuspended in 2× Laemmli sample buffer (Bio-Rad) supplemented with 5% 2-mercaptoethanol (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2024Quote: ... This mixture was transferred to a 2 mm ice cold electroporation cuvette (Bio-Rad). Transformation of WT P ...
-
bioRxiv - Microbiology 2024Quote: ... The assays were incubated for 2 hours at 37 °C in a thermocycler (Biorad). Samples were then filtered through 3 kDa filter plates and derivatized with 4,5-dimethoxy-1,2-phenylenediamine hydrochloride (DMB) ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins bound to Dynabeads were eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis ...
-
bioRxiv - Microbiology 2023Quote: ... Xrn-1 digest samples were diluted 1:1 in 2X RNA loading dye (BioRad) and RNA was denatured at 80°C for 90 seconds ...
-
bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
-
bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
-
bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
-
bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
-
bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
-
bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
-
bioRxiv - Bioengineering 2019Quote: Proliferation of MSCs was evaluated by determining the total cell count per well (n=3 wells/treatment/time-point) using a TC20 Automated Cell Counter (Bio-Rad).
-
bioRxiv - Cell Biology 2019Quote: Home-made gels: The home-made Laemmli resolving gel (10 ml) contains 3 ml of 40% Acrylamide/Bis (cat#: 1610148, Bio-Rad), 2.5 ml of 1.5M Tris-HCl pH 8.8 (cat# ...