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Citations for Bio-Rad :
1551 - 1600 of 1856 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Cell Biology 2023Quote: ... Signals from HRP-conjugated secondary antibodies were detected by enhanced chemiluminescence (SuperSignal® Pico or Femto, ThermoScientific) using ChemiDoc (Biorad). Antibodies used in this study are listed in Table S6.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... Blots were rinsed and probed in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000; BioRad, Hercules, CA, USA) for 30 min at RT in 2.5% NFDM or BSA in 1× TBST ...
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bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
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bioRxiv - Cell Biology 2023Quote: ... And then blots were probed with secondary antibodies for 1h at room temperature before ECL development and imaging (Bio-Rad). The primary antibodies used for immunoblotting are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The appropriate HRP-conjugated secondary antibodies were used for the chemiluminescent detection of proteins with Clarity Max Western ECL Substrate (BioRad). Membranes were scanned with a ChemiDoc imaging system (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with horseradish-peroxidase-linked secondary antibodies diluted 1:5000 followed by exposure to Clarity Western ECL Substrate (Cat. No. 170-5060, BioRad) and film development ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody cocktail against CD68 (rat monoclonal; 1:250; catalog no. MCA1957; Bio-rad), GFAP (Chicken polyclonal ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies conjugated with alkaline phosphatase were used with an ImmunStar™-Alkaline Phosphatase (AP) Western Chemiluminescent Kit (Bio-Rad) for signal development ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with an Alkaline Phosphatase-conjugated Anti-Digoxigenin antibody and the chemiluminescent signal was revealed using a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... the membrane was treated with HRP-conjugated secondary antibody and later stained and imaged for chemiluminescence using freshly prepared Clarity ECL substrate (Biorad). All information regarding the reagents has been provided in Table S3.
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary horseradish peroxidase linked antibody was incubated for 1 hour with gentle agitation at room temperature and developed using chemiluminescence blotting substrate (1705061, BioRad or 34095 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed 3X in PBST (10 minutes) and incubated for 1 hour with horseradish peroxidase conjugated anti-rabbit secondary antibody (BioRad 170-6515 ...
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bioRxiv - Biochemistry 2024Quote: ... We utilized colorimetric detection to visualize the apoAI-EfpA band(s) on the membrane after WB transfer: The primary mouse antihistidine tag antibody (BioRad) and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... in a wet tank apparatus, probed with the indicated primary antibodies, and detected with ECL (ECL Select, Cytiva #RPN2235) in a ChemiDoc XR (BioRad). Images were analyzed with ImageLab software (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... blots were washed twice in TBS with 0.05% Tween 20 and then incubated with a goat α-rabbit secondary antibody conjugated to horseradish peroxidase (Biorad) at a 1:5 000 dilution in TBS with 0.05% Tween 20 and 5% milk for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Molecular Biology 2024Quote: ... or HRP-labelled secondary antibodies and scanned in an Odyssey Infra-red Imaging System (Li-COR) or a Chemidoc MP imaging system (BioRad). Images were analyzed and quantified with ImageStudio software (LI-COR).
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with respective peroxidase-conjugated secondary antibodies and signals were detected using chemiluminescence with a ChemiDoc MP Imaging System (Biorad). Antibodies and dilutions are listed in Supplementary Table 2.
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with fluorescent tagged secondary antibodies for 45 minutes at RT followed by imaging on a fluorescence scanner imaging system (Biorad).
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bioRxiv - Biophysics 2019Quote: ... and horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) antibody (catalog no. 1721011, batch no. 64109318) were all purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
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bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... for the rapamycin blot or with the fluorescent StarBright Blue 700 anti-mouse (1:5000) and StarBright Blue 700 anti-rabbit (1:5000) secondary antibodies (Bio-Rad) for the MRT68921 blots in TBS-T containing 5% powder milk ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes incubated with HRP (horseradish peroxidase)–conjugated antibodies (HRP rabbit, 111 035 144; HRP mouse, 115 035 146) were developed using Clarity western ECL substrate solutions (Bio-Rad) with a ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Immunology 2021Quote: ... Macrophages and T cells in aortic root were stained by incubating aortic root cross-sections with the primary antibodies to CD68 (Bio-Rad) and CD3 (Abcam) ...
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bioRxiv - Microbiology 2021Quote: ... the membranes were washed 3 times with 1 x TBS-T and antibody binding was detected in a BioRad Versa Doc System with the Quantity One software (BioRad, Germany) by chemiluminescence using the Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... CldU or IdU were detected by immunofluorescence with either anti-BrdU antibody that recognizes CldU (MCA2060, Bio-Rad Laboratories, Hercules, CA) or anti-BrdU antibody that recognizes IdU (347583 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed thrice with TBS-T before incubation for one hour at room temperature with secondary antibodies (horse radish peroxidase goat anti-mouse or anti-rabbit; Bio-Rad), all at 1:5000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... A gate was established for each factor using negative control samples stained with mouse IgG1 negative control antibodies (BIO-RAD, MCA928) or polyclonal rabbit IgG (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins of interest were detected using an HRP-conjugated secondary antibody and ECL substrates (Amersham) and visualized with the ChemiDoc MP system (Bio-Rad). Rabbit IgG heavy chains of anti-ATG12 antibody overlap with the ATG12-ATG5 conjugate ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were incubated with HRP-conjugated secondary antibodies for both FLAG and GFP stainings (Rabbit anti-goat-HRP conjugate, #1721034, Bio-Rad) diluted at 1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using Clarity™ Western ECL Blotting Substrate (BIO-Rad).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Bioengineering 2019Quote: ... The anti-SSTR2 mAb was purified using our two-step antibody purification protocol by the NGC system (Bio-Rad, Hercules, CA) equipped with Protein A column and ion exchange column.(34 ...
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bioRxiv - Physiology 2019Quote: ... paraffin-embedded sections were incubated at 4°C overnight with antibodies for F4/80 (MCA497G, Bio-Rad Laboratories, Hercules, CA, USA), HILPDA (sc-137518 HIG2 Antibody (C-14) ...
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bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
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bioRxiv - Genetics 2019Quote: ... Global DNA methylation was detected using specific antibodies and was quantified colorimetrically by reading the absorbance at 450 nm in a microplate spectrophotometer (Bio-Rad Microplate Reader ...
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bioRxiv - Physiology 2020Quote: ... Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules, CA). Antibodies against ERα (F-10 mouse – for WB) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred onto PVDF membrane and probed with primary antibody (Table 1) at a dilution of 1:1000 prepared in 5% blotting grade blocker (Bio-Rad) for 12-16 h at 4 °C ...