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Citations for Bio-Rad :
1501 - 1550 of 1723 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The oligonucleotide substrate products were visualized using the ChemiDoc MP Imaging System to detect Cy 5 fluorescence (Bio-Rad, 1708280).
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bioRxiv - Bioengineering 2023Quote: ... the blots were washed as before and incubated with Supersignal west pico chemiluminescence substrate (Pierce) and developed using ChemiDoc XRS+ (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... The signal was detected by enhanced chemiluminescence reagents (Immobilon Crescendo Western HRP substrate, Merk) was imaged with a ChemiDoc MP Imaging System (Biorad). Ponceau S staining (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed and blocked in 1x TBST with 5% bovine serum albumin before consecutive incubation with primary antibody (see Suppl. Table 3) and horseradish peroxidase-coupled protein A (Cymed) and detection using Clarity Western ECL Substrate (BioRad).
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bioRxiv - Plant Biology 2023Quote: ... The chemiluminescence signal was detected with the PierceTM ECL Plus Western Blotting Substrate on a GelDoc Go Imaging (BIO-RAD).
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bioRxiv - Cell Biology 2023Quote: ... The appropriate HRP-conjugated secondary antibodies were used for the chemiluminescent detection of proteins with Clarity Max Western ECL Substrate (BioRad). Membranes were scanned with a ChemiDoc imaging system (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with horseradish-peroxidase-linked secondary antibodies diluted 1:5000 followed by exposure to Clarity Western ECL Substrate (Cat. No. 170-5060, BioRad) and film development ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed in 4 x 5 minutes in 0.2% TBS tween before incubating in Clarity™ Western ECL Substrate (BioRad) for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... in blocking solution for 1 hour at room temperature, then visualized by addition of ECL substrate (Amersham, GE) and imaged using a ChemiDoc (BioRad). Western blot quantification was performed in ImageLab (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... The blots were finally washed thrice with TBST with 5 minute incubations before adding the ECL substrate solution (1705061, Biorad). Chemiluminescence images were acquired on a Chemidoc™ imaging system (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed again with TBS-T 3 times and the membrane was visualized with Vilbert Lourmat imaging system following an incubation with enhanced chemiluminescence (ECL) substrate (Biorad).
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary horseradish peroxidase linked antibody was incubated for 1 hour with gentle agitation at room temperature and developed using chemiluminescence blotting substrate (1705061, BioRad or 34095 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein bands were visualized via chemiluminescence (ECL Enhanced Chemo Luminescence; Pierce ECL Western Blotting Substrate) using the ChemiDoc MP system (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... strips were developed with SuperSignal West Dura Extended Duration Substrate and imaged on a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Developmental Biology 2023Quote: ... the membranes were washed three more times with 1X - TBST and treated with Clarity™ Western ECL substrate (Bio-Rad) for protein visualization.
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bioRxiv - Biophysics 2023Quote: ... the membrane was treated with HRP-conjugated secondary antibody and later stained and imaged for chemiluminescence using freshly prepared Clarity ECL substrate (Biorad). All information regarding the reagents has been provided in Table S3.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were treated with SuperSignal West Pico Plus chemiluminescent substrate (ThermoScientific, 34577) and imaged with a ChemiDoc imaging system (BioRad). Antibodies used ...
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bioRxiv - Neuroscience 2023Quote: ... HRP is detected by a chemiluminescence assay using the kit Clarity Western ECL Substrate (Bio-Rad, Marnes-la-Coquette, France), whose light emission is captured by the camera of the device ChemiDoc Touch (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... Digital ECL substrate solution (Kindle Biosciences) was added to the membrane before detection with a ChemiDoc MP Imaging System (BioRad). The dot blot intensity was quantified using ImageJ v1.51g 87.
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed in TBST and imaged using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo) in a Chemidoc (Bio-Rad) and analyzed using ImageJ (www.imagej.nih.gov) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed three times in TBS/0.05% TritonX-100 and developed using Clarity Western ECL Substrate (Bio-Rad, 1705061). Blots were imaged on an Amersham Imager 680 ...
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bioRxiv - Cancer Biology 2024Quote: ... The HRP-coupled secondary antibodies were used at a 1:10,000 dilution followed by development using ECL substrate from Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... the signal was developed using Clarity Western ECL Substrate and visualized on the ChemiDoc MP Imaging System (both Bio-Rad).
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bioRxiv - Plant Biology 2024Quote: ... Blots were washed four times with PBS-T for 5 minutes and chemiluminescent signals were detected using Clarity Western ECL Substrate (BioRad,), and visualised using the ImageQuant® LAS-4000 imager (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... HRP conjugated secondary antibodies against appropriate species were used and signal developed by using Clarity Western ECL substrate (Biorad, USA). For co-immunoprecipitation ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were finally washed another three times in 1X TBS-T and developed with Clarity Max ECL Substrate (BioRad, 1705062). Image acquisition and quantification were conducted with BioRad ChemiDocTM MP Imaging System and software (Image Lab 6.0.1) ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibodies were used at 1:5000 dilutions and chemiluminescence signal was produced using the Clarity western ECL substrate (BioRad) and detected using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min and visualized using the chemiluminescent setting on a ChemiDoc XRS+ (Biorad).
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bioRxiv - Microbiology 2024Quote: ... Membranes were revealed with Bio-Rad Clarity Max Western ECL substrate and imaged using the ChemiDoc MP System (Bio-Rad).
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bioRxiv - Immunology 2024Quote: ... and incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min before visualization using the chemiluminescent setting on the ChemiDoc XRS+ (Biorad).
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bioRxiv - Cancer Biology 2021Quote: ... An anti-mouse IgG secondary antibody (diluted 1:5000) conjugated with horseradish peroxidase (HRP) was used to reveal the immunoblot bands by chemiluminescence (Clarity Western ECL substrate, Bio-Rad) following the supplier’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes incubated with HRP (horseradish peroxidase)–conjugated antibodies (HRP rabbit, 111 035 144; HRP mouse, 115 035 146) were developed using Clarity western ECL substrate solutions (Bio-Rad) with a ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... The amount of protein bound at each capture spot was detected using chemiluminescent substrate and the signal was captured using a ChemiDoc imaging system (Bio-Rad). Intensity of the capture spots were quantified using ImageJ.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins of interest were detected using an HRP-conjugated secondary antibody and ECL substrates (Amersham) and visualized with the ChemiDoc MP system (Bio-Rad). Rabbit IgG heavy chains of anti-ATG12 antibody overlap with the ATG12-ATG5 conjugate ...
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bioRxiv - Microbiology 2020Quote: ... anti-GFP antibody was used and bound primary antibody was detected using anti-rabbit IgG-HRP secondary antibody following visualization with the Clarity™ Western ECL Substrate (BioRad) and subsequent detection in a ECL Chemocam Imager (Intas Pharmaceuticals Limited) ...
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bioRxiv - Cancer Biology 2019Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using Clarity™ Western ECL Blotting Substrate (BIO-Rad).
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bioRxiv - Genetics 2019Quote: ... membranes were washed three times for five minutes each in TBST and protein detected using Clarity western ECL substrate (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The chemiluminescence of the membranes was developed using Super Signal Femto Substrate Kit (Pierce) and developed using ChemiDoc Touch Imager (Bio-Rad). Primary antibodies and conditions used are as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK). Spots were enumerated using an AID ELISpot reader and software (AID).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Bioengineering 2021Quote: ... Both secondary antibodies are HRP-conjugated and were visualized using West Pico Plus HRP substrate (Thermo Fischer) and measured with a ChemidoC XRS image analyzer (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed three times with PBS-T (15 min, RT) and chemiluminescence was detected after 10 min incubation with freshly prepared Clarity Western ECL Substrate (Bio-Rad) with a LAS-4000 gel scanning station (Fujitsu Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... The chemiluminescent developing reagents were Amersham ECL− (Cytiva Inc., for U2AF2 or GAPDH) or Clarity blotting substrate (Bio-Rad, for H2B), detected on a Chemidoc− Touch Imaging System (Bio-Rad Lab ...
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bioRxiv - Neuroscience 2021Quote: ... the membrane was washed and submerged in chemiluminescent HRP substrate while the fluorescent signals of the blots were captured by gel imaging analysis system (Bio-rad). The membrane was incubated with primary antibody of β-actin (1:1000 ...