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Citations for Bio-Rad :
1501 - 1550 of 3363 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 4995 µL of cell lysis buffer and 20 µL of PMSF 500 mM (Bio-Plex® Cell Lysis Kit, Biorad, #171304011). The lysate was frozen at −80°C for 2 minutes and then ...
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bioRxiv - Immunology 2023Quote: ... Sorted live CD14+CCR2+ and CD14+CCR2- cells were counted in triplicates on a TC20 Automated Cell Counter (BioRad, Hercules, California), washed ...
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bioRxiv - Genomics 2023Quote: Pooled blood cells from 9 adults were counted and their viability was assessed using an automatic cell counter (Bio-Rad TC20) with Trypan Blue staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Total cell counts from each condition were collected using a Bio-Rad TC20 automated cell counter (Bio-Rad, Hercules, CA, USA). Second ...
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bioRxiv - Microbiology 2023Quote: ... Digested tissue was then forced through a 70 μm filter and single cells were counted using a C20 Automated Cell Counter (Bio-Rad). For staining 3×106 cells were plated in round bottom 96 well plates.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in FACS buffer for flow cytometric analysis on a ZE5 cell analyzer (Bio-Rad, Hercules, CA) or a Novocyte Quanteon (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stained with Trypan blue and live cells were counted in two technical replicates with a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... PBMCs isolated from two donors were infected with GFP-EBV for 28 days in 6-well plates and subsequent B-cells blasts were photographed using using a ZOE Fluorescent Cell Imager (BIO-RAD). Using similar strategy ...
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bioRxiv - Immunology 2023Quote: ... Samples were concentrated afterwards by centrifugation at 300 × g for 10 min at 4°C and resuspended in 1 × PBS with 0,05% BSA for further assessment of cell numbers and viability with the TC20™ Automated Cell Counter (Bio-Rad). Samples balanced by responders and nonresponders for each treatment were mixed in pools of 8 patients at a 50:50 ratio and concentrated by centrifugation in an appropriate volume of 1 × PBS-0.05% BSA to obtain a final cell concentration > 4,000 cells/µL ...
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bioRxiv - Cell Biology 2024Quote: The cells in suspension were counted and examined for viability with trypan blue staining using a TC-20 cell counter (Bio-Rad). Approximately 16,500 live cells at 1×106 cells/ml concentration with 65% or above viability were loaded into the Single Cell Chip to generate single cell emulsion in Chromium Controller with Chromium Single Cell 3’ Library & Gel Bead Kit v3.1 (10x Genomics ...
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bioRxiv - Biophysics 2024Quote: ... Growth media was exchanged after 24 hours and cells were checked for protein expression using a fluorescent cell imager (Bio-Rad). Cells were harvested after another 24 hours in cold PBS before purification ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification of the relative protein expression in ΔPr-A9 cells compared to the WT cells was determined in Image Lab software (Bio-Rad). The average signal of duplicate spots subtracting background value from negative control spots represented the levels of each protein ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated using appropriate HRP secondary antibodies (donkey anti-rabbit 711-035-152, donkey anti-mouse 715-035-150, Jackson Immunoresearch Laboratory and Biorad ChemiDoc XRS System) or fluorescent secondary antibodies (IRDye 800 CW ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Then ran the assay in accordance with the specification (Bio-Plex Pro Mouse Cytokine Grp, Catlog NO. #M60009RDPD, LOT. 64212941, Bio-Rad Laboratories, Inc., USA). Read the plate at Bio-Plex MAGPIX System (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... while insulin levels were assayed using the quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plate was washed 3 times prior to adding 50 μl of 1:1000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Bio-Rad, Cat# 172-1011) to the plate and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed three times for 5 min with 1x TBST and incubated with either an anti-mouse IgG- HRP-conjugate (1:5000, Bio-Rad Cat#172-1011) or an anti-rabbit IgG-HRP-conjugate (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 h with either anti-mouse or anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Bio-Rad Laboratories, Hercules, CA). Bands were visualized and subjected to densitometric analysis using an Odyssey Fc system (LI-COR Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at 70°C. The following primary antibodies were used (see also Suppl. Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-DYKDDDDK tag primary antibodies (Wako, 1E6, 1:5,000 dilution) combined with anti-mouse-HRP secondary antibodies (Bio-Rad, #1706516, 1:5,000 dilution). SigA proteins were detected using anti-SigA85 (1:10,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP (anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase, Bio-Rad, 1:5,000 dilution) and detected using the Clarity Western enhanced-chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Molecular Biology 2019Quote: Cell cycle duration of hTERT-RPE cells was estimated by passaging the cells and counting the number of seeded and harvested cells over a period of 24 and 48 hours (Bio-Rad, TC20).
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bioRxiv - Bioengineering 2019Quote: ... and the electrophoresis was carried out for 30 min at 50 mA in a horizontal gel electrophoresis apparatus (Sub-Cell GT Cell, BioRad, Cressier, Switzerland) using acetate buffer (as above ...
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bioRxiv - Immunology 2021Quote: ... The cells were centrifuged at 300G for 7 minutes at 4°C and the cell pellet was resuspended then counted with the BioRad cell counter (Bio-Rad, TC20).
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Immunology 2022Quote: ... The number of the total cells isolated from the spinal cord was counted by TC20 Automated Cell Counter (Bio-Rad, Hercules, CA), and the number of spinal cord-infiltrating TCR Vα3.2+ 2D2 CD4+ T cells and TCR Vα3.2- non-2D2 CD4+ T cells were calculated based on the percentage obtained by flow cytometry.
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bioRxiv - Genetics 2022Quote: ... resuspended to 10 million cells/ml and sorted on a two-laser (466 nm and 561 nm) cell sorter (Bio-Rad S3) as previously described (55) ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected in a 1.5 mL microtube and 10 µL of the suspension was used for live cell counting using the trypan blue exclusion assay with a TC20™ automated cell counter (Bio-Rad). BSA (0.05 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: 5 μg DNA/106 cells or 100 nM siRNA /106 cells were transfected by electroporation (200 mV, 950 μF and 480 Ω; Bio-Rad). Expression was measured 24-48 h post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... the diameter of live cells was counted using trypan blue staining and a TC20 automated cell counter (Bio-Rad, Hercules, CA, USA). Cell sizes were analyzed using the ImageJ software (National Institutes of Health ...
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bioRxiv - Genomics 2023Quote: ... Before sequencing the cells were counted and tested again for viability using a TC20 automated cell counter (Bio-Rad, Hercules, California, USA).
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bioRxiv - Cancer Biology 2023Quote: To ensure the treatments used in this work did not interfere with cell viability cells were stained with Trypan Blue (BioRad, cat.#1450011) 48 h after transfections with siRNA or after 24 h of treatment with MEL23 or vehicle ...
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bioRxiv - Microbiology 2024Quote: ... 100-200 ng of DNA was added to 50 uL of concentrated cells and pulsed using the Gene Pulser 541 X cell electroporation system (Bio-Rad #1652660) at 2500 V ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were isolated from cell homogenate by centrifugation and counted on an automated cell counter (TC20 BioRad, range 4-6 um). Transposition of 100k (weeks 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... staining using the TC-10 Automated Cell Counter (BioRad). Comparisons were made by paired t-test ...
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bioRxiv - Microbiology 2021Quote: ... using TransBlot semi dry transfer cell (Bio-Rad, Germany). After saturation of non-specific protein binding using 5% low-fat milk diluted in TBS-T at rt for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... 2 million N2A cells were transfected using TransFectin (Biorad) with either CFP-ctrl. ...
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bioRxiv - Molecular Biology 2020Quote: ... using Mini Trans-Blot cells (Bio-Rad Laboratories, 1703930) at 80 V for 2.2h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... using a Gel Transfer Cell (BioRad catalogue number 1703930). Primary antibodies were used at 1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were transferred to the electroporation cuvette (1652088, BioRad), and transfected using a Gene Pulser Xcell electroporation system (1652661 ...
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bioRxiv - Physiology 2019Quote: ... by wet electroblotting (Mini Trans-Blot® Cell, Biorad) at 120 V for 2,5 h ...
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bioRxiv - Bioengineering 2019Quote: ... The ZOE™ Fluorescent Cell Imager (Bio-Rad Laboratories) was used to observe growth behavior with multiple time intervals.
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bioRxiv - Immunology 2021Quote: ... counted using a TC20 automated cell counter (Bio-Rad) and cell number across samples was equalized ...
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bioRxiv - Cancer Biology 2020Quote: ... Data were collected on the ZE5 cell analyzer (Biorad) and analyzed with FlowJo™ Software v10.
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bioRxiv - Cancer Biology 2020Quote: ... Data were collected on the ZE5 cell analyzer (Biorad) or the LSRFortessa™ (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Data were collected on the ZE5 cell analyzer (Biorad) and analyzed with FlowJo™ Software v10.
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bioRxiv - Cell Biology 2021Quote: ... counted with a T20 automated cell counter (Bio-Rad), and resuspended in the complete medium ...
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bioRxiv - Microbiology 2020Quote: ... cells collected and boiled in Laemmli sample buffer (BioRad) containing 1% 2-Mercaptoethanol (Sigma Aldrich) ...