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Citations for Bio-Rad :
1501 - 1550 of 1949 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... the cells were divided into 100 μl aliquots and heated individually at different temperatures for 3 minutes (Thermal cycler, BIO-RAD) followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Each gradient was then separated into several fractions (i.e., bottom-to-top) of equal volumes (about 100 μl) using a fraction collector (BioRad, Hercules, USA). RNA from all fractions was prepared following standard TRI Reagent® protocols (Molecular Research Center Inc. ...
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bioRxiv - Immunology 2019Quote: ... to nitrocellulose membranes using a constant voltage of 100 V for 2 h using the BioRad Midi PROTEAN Western blotting module according to the manufacturer’s instructions (BioRad, CA, USA). The membranes were blocked with blocking solution [Tris-buffered saline (TBS ...
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bioRxiv - Genetics 2021Quote: ... according to the instructions of the manufacturer or extracted using Chelex® 100 resin (Bio-Rad Laboratories Inc., Hercules, CA USA). Two final elutions of DNA were made from the kit extractions with the first being used for construction of genomic libraries for a related study and the second being used for screening of Vssc mutations after being diluted 1:5 with water ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then analyzed using flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories, Inc.). Standard curves were generated by Bio-plex Manager software to determine unknown sample concentration ...
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bioRxiv - Neuroscience 2020Quote: ... The bead sets were analyzed using a flow-based Luminex™ 100 suspension array system (Bio-Plex 200; Bio-Rad Laboratories). A five-parameter model was used to calculate final concentrations ...
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bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
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bioRxiv - Immunology 2020Quote: ... 1:400) to identify αβT cells and mouse anti-pig SWC5 (clone b37c10, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of γδT cell subpopulation ...
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Immunology 2022Quote: ... 10 mM sodium bicarbonate in iron-free HEPES-buffered saline (HEPES 20 mM, NaCl 150 mM, treated with Chelex-100 chelating resin [Bio-Rad, Hercules ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Plant Biology 2022Quote: ... Infiltrated leaves were infiltrated with 100 µM D-luciferin and LUC activity was quantified after 48 h using a low-light CCD imaging apparatus (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Microbiology 2023Quote: ... qPCRs were performed on 1 μl diluted cDNAs in 10 μl scale in technical duplicates amplifying regions of 100 – 150 bp with the iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions using primers indicated in Table 8 ...
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bioRxiv - Microbiology 2023Quote: ... The qRT-PCR reaction was set up in a 20-μl volume with 100 - 200 ng of RNA and was conducted using the CFX Connect Real-Time PCR System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... an equal number of cells were lysed by resuspension in an equal volume (100 μL per 1x106 cells) of hot Laemmli buffer (Bio-Rad). If cells could not be counted upon collection ...
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bioRxiv - Microbiology 2023Quote: ... Lactose and salts were removed by gel filtration chromatography over a BioRad P2 column (100 cm x 316 mm, Bio-Rad) using a semi-automated fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2022Quote: ... 5% sorbitol synchronized ring stage Pf NF54 parasites at 5-8% parasitemia were electroporated with 100 µg of pFC-LINUP plasmid at 0.31 kV and 950 µF using a BioRad Gene Pulser (BioRad, Hercules, CA). Positive selection with 8 nM WR99210 (WR ...
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Physiology 2023Quote: ... and the cells were resuspended in permeabilization buffer consisting of intracellular-like medium (120mM KCl; 10mM NaCl; 1mM KH2PO4; 20mM HEPES; pH 7.2) that had been cleared with Chelex 100 (Bio-Rad # 1422822) to remove trace Ca2+ ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Physiology 2023Quote: ... and the pellet resuspended in 37.5µL of Na+-free intracellular-like medium (120mM KCl; 1mM KH2PO4; 20mM HEPES; pH 7.2) that had been cleared with Chelex 100 (Bio-Rad # 1422822) to remove trace Ca2+ ...
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bioRxiv - Physiology 2023Quote: ... and the pelleted cells resuspended in permeabilization buffer consisting of intracellular-like medium (120mM KCl; 10mM NaCl; 1mM KH2PO4; 20mM HEPES; pH 7.2) that had been cleared with Chelex 100 (Bio-Rad # 1422822) to remove trace Ca2+ ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was amplified using KAPA HiFi Hotstart ReadyMix PCR kit (Nippon Genetics) with T-100 Thermal Cycler (Biorad, Hercules, CA). The temperature profile of the reaction was 95 °C for 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... 80µl of ice-cold bacterial cells were mixed with 1µl plasmid (pBAD28 == pEMPTY or pHND10 == pAPY, 100-200ng, key resources table) in chilled 0.1cm cuvettes (Bio-Rad, cat# 1652089) and immediately electroporated (Voltage = 1.8kV ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Systems Biology 2020Quote: ... equipped with a Hitrap™ column of 5 mL (Bio-Rad, Hercules, CA, USA) was used to purify produced proteins ...
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bioRxiv - Immunology 2021Quote: ... Protein 1ug/ml were immobilized onto NC membranes (0.22-m pore size; Bio-Rad). The membrane kept for drying for 5-10 min ...
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bioRxiv - Biochemistry 2019Quote: ... the beads were transferred to a gravity-flow Econo column (49 ml volume; BioRad), washed with five column volumes (CV ...
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bioRxiv - Microbiology 2019Quote: ... washed with column buffer and then transferred to an empty column (BioRad 15 ml). Unbound protein was washed using until background OD280 reached ~0.06 ...
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bioRxiv - Biochemistry 2020Quote: ... and loaded onto with a volume of 10 mL column (Bio-Rad, Lab., Inc.) and buffer exchange done manually ...
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bioRxiv - Biochemistry 2020Quote: ... RNAP was then loaded on a 50 ml Bio-Rex 70 column (Bio-Rad) equilibrated with Bio-Rex buffer (10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... protein-bound beads were transferred to a 10-mL chromatography column (Bio-Rad 7311550) and washed with the following buffers ...
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bioRxiv - Genomics 2022Quote: ... and suspended to 106 cells/mL in PBS + 0.05% BSA (Bio-Rad cell counter) (Figure 1).
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and loaded onto a 2 ml CHT2-I hydroxyapatite column (Bio-Rad) equilibrated in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... 15525-017)] and 900 mL ddH2O] and transferred to Polyvinylidene fluoride membranes (Bio-Rad, 162-0177) at 85 V for 1 hour in 1 X SDS-PAGE Transfer Buffer [100 mL of 10 × 1L Stock [30.275 g Tris Base (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... the matrix was transferred to a gravity-flow Econo column (49 ml volume; BioRad) and washed with buffer D [50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were transferred to 10 ml columns (Poly-Prep Chromatography Column, Bio-Rad) and eluted with LFB1/50 supplemented with 250 mM imidazole.
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bioRxiv - Plant Biology 2021Quote: ... and 1 ml Luminol/enhancer solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and observed using ChemiDoc (ChemiDoc™ Imaging Systems ...