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Citations for Bio-Rad :
101 - 150 of 3384 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 10-30 ug total protein were resolved by SDS-PAGE and transferred to PVDF membranes (Bio-Rad). Primary antibody incubations were done overnight with antibodies diluted in 3% BSA in TBS-T (Tris-buffered saline/Tween 20) ...
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bioRxiv - Bioengineering 2024Quote: Purified antibodies (0.5 ug each) were denatured by adding 4X LaemmLi sample buffer (Bio-Rad, cat.: 1610747) and 2-mercaptoethanol and heating at 100°C for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR reactions were set up with final 1x iQ SYBR Green supermix (BioRad), 400 nM each of Fw and Rev QPCR primers (see Table 4) ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR reactions were set up using iTaq Universal SYBR Green Supermix (Bio-Rad) with triplicates of 10 µl per reaction ...
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bioRxiv - Immunology 2019Quote: ... qPCR reactions were set up using SSOFast EvaGreen Supermix (Bio-Rad, cat. #: 1725200) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... The reaction was set up on ice and run in a thermocycler (BioRad), using a touchdown PCR program ...
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bioRxiv - Microbiology 2020Quote: ... Two LAMP assay reactions were set-up in CFX-96 (Bio-Rad, USA) for each sample with SARS-CoV-2 LAMP primer mix and internal control LAMP primer mix ...
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bioRxiv - Developmental Biology 2022Quote: ... and qPCR reaction was set up using iTaq Universal SYBR Green Supermix (BioRad) per manufacturer’s instructions with the primers listed in Table 2 ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... qRT-PCR reactions were set up using SsoAdvanced SYBR green (Bio-Rad, #1725270) on CFX Opus 96 (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... qPCR reactions were set up using iQTM SYBR Green Supermix (Bio-rad, 1708884), and run on the CFX ConnectTM Real-Time System ...
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bioRxiv - Cell Biology 2020Quote: ... 40% acrylamide and 2% bisacrylamide stock solutions were purchased from Bio-Rad (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... and bis-acrylamide (Bis) (stock 2 %, #1610142, Bio-Rad Laboratories, Hercules, USA) with PBS in 15 ml falcon tube [65] ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were diluted using 1X Laemmli Sample Buffer (4X stock, BioRad, 1610747) with 10% β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of Spike transcripts was quantified by quantitative real-time PCR (qPCR) using CFX384 machine (BioRad) with a fluorescence reporter (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extract (4 ug per lane) was separated on a 10% TGX Stain-Free polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Systems Biology 2022Quote: Cell extracts (15 ug) were resolved by SDS-PAGE using 4-15% TGX precast gels (Bio-Rad #4561086) with tris/glycine/SDS buffer and transferred onto PVDF membranes using the Bio-Rad Mini-PROTEAN Electrophoresis System ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 ug of protein was loaded onto 4-15% Mini-Protean TGX (4561084, Bio-Rad, Hercules, CA) gels ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was synthesized from 0.1 ug of aphid RNA using the iScript cDNA Synthesis Kit (Bio-Rad). PLRV titer in undiluted cDNA was quantified by digital drop PCR using EvaGreen Supermix and the QX100 droplet digital PCR system as previously described (51).
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bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
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bioRxiv - Genetics 2023Quote: ... 8.5 ug of each protein sample was loaded onto a 4–20% Stain-free Protein Gel (Biorad #5678093) with a dual-fluorescent ladder (Biorad #1610374) ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL casein (Blotting-Grade Blocker, Biorad), 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mL of chemiluminescent substrate (#1705061, Biorad) was applied on the membrane and visualized by Amersham Imager 680.
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bioRxiv - Genomics 2022Quote: ... The reaction was cleaned up by ethanol precipitations and P4 beads (Bio-Rad, #1504124) spin columns ...
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bioRxiv - Immunology 2022Quote: ... qPCR reactions were set up using SSOFast EvaGreen Supermix (Bio-Rad, cat. no. 1725200) or Fast SYBR Green master mix (Applied Biosystems cat ...
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bioRxiv - Microbiology 2022Quote: ... precipitated in absolute ethanol and cleaned-up with Micro Bio-Spin Chromato Column (Biorad) (52) ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR reactions were set up using SsoAdvanced Universal SYBR Green Supermix (Biorad, 1725271) and run in triplicates using a QuantStudio 6 system (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was cleaned up by P-6 Micro Bio-Spin Column (Bio-Rad). For fluorophore labeling ...
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bioRxiv - Bioengineering 2022Quote: ... 0.06% SDS (5% w/v stock in DI water, 161-0301, Bio-Rad), 0.06% Triton X-100 (5% w/v stock in DI water ...
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bioRxiv - Bioengineering 2022Quote: ... 0.035-0.25% N,N-methylenebisacrylamide crosslinker (Bis, 2% w/v stock, 161040, BioRad), 0.06% SDS (5% w/v stock in DI water ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl of sequin spike (https://www.sequinstandards.com) was added to 14 μl of RNA for cDNA synthesis with SsoAdvanced iScript (Bio-Rad), followed by RT-qPCR using SsoAdvanced master mix (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates for quantitative Spike western blotting were loaded onto TGX Stain-Free 4-20% gradient gels (Bio-Rad, 4568094) (Fig ...
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bioRxiv - Neuroscience 2019Quote: ... were heated at 99 °C for 3 minutes and subjected to 4-20% gradient SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad Laboratories, Inc.). Membranes were then blocked for 1 hour with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... heated in water bath for 5 minutes at 99°C and analysed by SDS-PAGE using Any-kD Mini-PROTEAN TGX Stain-Free gels (Bio-Rad, California, USA) in a 2-minute run for sample clean-up purposes ...
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bioRxiv - Genomics 2020Quote: ... 30 ug of each extract was resolved through 4-20% Criterion Tris-Glycine (TGX) Stain-Free SDS-PAGE (Biorad) and transferred to nitrocellulose ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Immunology 2022Quote: ... 30 ug of Protein extracts were separated by 4-12% Bold NuPage (ThermoScientific) and transferred onto PVDF membranes (BioRad). After blocking with 3% albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 ug of protein was loaded per lane in a pre-cast gel for SDS-PAGE (Bio-Rad). Gel was then transferred using the TurboBlot Transfer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Molecular Biology 2020Quote: ... The adaptor was cleaned up on a Micro Bio-Spin 6 chromatography column (Bio-Rad) equilibrated with water ...
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bioRxiv - Biochemistry 2022Quote: ... accordingly to manufacture’s specifications and heated up in sealed qPCR stripes (Bio-Rad, Hercules, California) from 20°C to 95 °C in C1000 Thermal Cycler CFX96 Real-time System (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... Real-time PCR reactions were set up using iTaq Universal SYBR Green Supermix (Bio-Rad) and were executed in a thermal cycler (CFX384TM Real-Time System ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR reactions were set up using the SYBR Green supermix containing the SsoAdvancedTM Polymerase (BioRad), 500nM forward and reverse primer and 1/1-40 diluted cDNA sample ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by Western blot as previously described.21 Densitometry was performed to quantify the cleavage efficiency of full-length spike to S1/S2 subunits using ImageLab 6.0.1 (Bio-Rad #12012931). The average results of two experiments were presented.
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Cancer Biology 2023Quote: ... KM12 or HCT116 cells (10 million) along with the spike-in control (20%) were carefully resuspended in cell suspension buffer (BioRad). The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml of 10% SDS (cat#: 1610416, Bio-Rad), 50 µl of 10% APS (cat# ...
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bioRxiv - Microbiology 2021Quote: ... 40 mg ml−1 blotting-grade blocker (Bio-Rad), and 5% (v/v ...