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Citations for Bio-Rad :
101 - 150 of 1383 citations for Oxychlordane Unlabeled 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 10 ug of total protein was loaded per lane onto a 12% SDS-PAGE gel (Bio-Rad), which was run at 100 V for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... 10-30 ug total protein were resolved by SDS-PAGE and transferred to PVDF membranes (Bio-Rad). Primary antibody incubations were done overnight with antibodies diluted in 3% BSA in TBS-T (Tris-buffered saline/Tween 20) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
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bioRxiv - Bioengineering 2024Quote: Purified antibodies (0.5 ug each) were denatured by adding 4X LaemmLi sample buffer (Bio-Rad, cat.: 1610747) and 2-mercaptoethanol and heating at 100°C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Biochemistry 2020Quote: A 10% Tris/Glycine/SDS Buffer solution was prepared with 100 mL buffer (Bio-Rad) in 900 mL tissue culture grade water ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of 100 μM protein samples was loaded on ENrich SEC 70 column (BioRad) in a buffer containing 50 mM phosphates (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: ... Detergents were removed by incubation with 100 mg/ml Bio-Beads SM-2 (Bio-Rad) for 1h at 23°C ...
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bioRxiv - Microbiology 2023Quote: Overnight cultures in TSB were diluted 100-fold into 1 mL of Chelex (Bio-Rad)- treated TSB with the addition of 25 μM zinc acetate ...
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bioRxiv - Microbiology 2024Quote: Overnight cultures in TSB were diluted 100-fold into 1 mL of Chelex (Bio-Rad)-treated TSB with the addition of 25 μM zinc acetate ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to PVDF membrane in 1x Transfer buffer (47.96 mM Tris, 39 mM Glycine, 0.038% SDS, 20% Methanol) using the Trans-Blot Turbo (BioRad) transfer system with up to 1.0 A ...
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bioRxiv - Neuroscience 2022Quote: ... the reaction was stopped by a final methanol-chloroform-H2O precipitation and resuspended in laemmli buffer (Bio-Rad) for WB analysis.
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bioRxiv - Neuroscience 2022Quote: ... The transfer sandwich was made using PVDF membrane (activated in methanol) in Tris-Glycine transfer buffer (Bio-Rad) with 20% methanol and transferred for 2 hours at 30V ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extract (4 ug per lane) was separated on a 10% TGX Stain-Free polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Systems Biology 2022Quote: Cell extracts (15 ug) were resolved by SDS-PAGE using 4-15% TGX precast gels (Bio-Rad #4561086) with tris/glycine/SDS buffer and transferred onto PVDF membranes using the Bio-Rad Mini-PROTEAN Electrophoresis System ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 ug of protein was loaded onto 4-15% Mini-Protean TGX (4561084, Bio-Rad, Hercules, CA) gels ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 ug of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was synthesized from 0.1 ug of aphid RNA using the iScript cDNA Synthesis Kit (Bio-Rad). PLRV titer in undiluted cDNA was quantified by digital drop PCR using EvaGreen Supermix and the QX100 droplet digital PCR system as previously described (51).
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA (1 ug RNA per reaction) using the iScript cDNA Synthesis Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... 8.5 ug of each protein sample was loaded onto a 4–20% Stain-free Protein Gel (Biorad #5678093) with a dual-fluorescent ladder (Biorad #1610374) ...
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bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
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bioRxiv - Physiology 2024Quote: ... 20 ug protein was separated in 4-20% Mini-Protean TGX Precast Gels (Bio-Rad, California, US, #4561094) and blotted using Bio-Rad Trans-Blot Turbo device (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1% SDS, pH ~8.6), wet transferred @ 100V for 60 min to methanol (Fischer, A412) activated PVDF (BioRad, 1620177) in Towbin transfer buffer containing (25 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were transferred to methanol-treated PVDF membranes using a Mini Trans-Blot Cell wet-transfer apparatus (BIO-RAD), according to manufacturer’s specifications at 400 mA for 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... gel at 150 V for 2 h and transferred onto a PVDF membrane at 25 V for 2 h in transfer buffer (3.03g Tris–HCl, 14.4g glycine, 20% methanol) using a semi-dry transfer apparatus (Bio-Rad). After transfer ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred by wet-blotting with Tris-Glycine buffer (250 mM Tris base, 1.92 M Glycine and 10% methanol) onto a nitrocellulose membrane (BioRad). After completion of the transfer ...
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bioRxiv - Immunology 2024Quote: ... pre-soaked in transfer buffer (20 mM Tris, 190 mM glycine, 20% Methanol, pH 8.3) using a Mini-Transfer cell (BioRad) for 1.5 hours (100 V constant ...
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bioRxiv - Microbiology 2020Quote: ... lipid vesicles (100 μg/ml) were immobilized to a neutravidin-coated NLC chip (Bio-Rad, USA) at a flow rate of 30 μl/min to a coupling level of about 1000 resonance units (RU) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.17 mg/ml Zymolyase 100 T (Nacalai) and poured into a plug mold (Bio-Rad). Agarose was allowed to solidify at 4°C for >30 min ...
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bioRxiv - Genomics 2020Quote: ... 30 ug of each extract was resolved through 4-20% Criterion Tris-Glycine (TGX) Stain-Free SDS-PAGE (Biorad) and transferred to nitrocellulose ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 ug) was converted to cDNA using the iScriptTM cDNA synthesis kit (# 1708891, BIO-RAD, Hercules, CA) following the manufacturer’s protocol (BIO-RAD ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 20 ug of acid-extracted protein was run in triplicate on a 4-20% SDS-PAGE gel (BioRad) and a single band corresponding to P1 and P2 was cut out for processing ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Immunology 2022Quote: ... 30 ug of Protein extracts were separated by 4-12% Bold NuPage (ThermoScientific) and transferred onto PVDF membranes (BioRad). After blocking with 3% albumin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 ug of protein was loaded per lane in a pre-cast gel for SDS-PAGE (Bio-Rad). Gel was then transferred using the TurboBlot Transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... using transfer buffer containing27mM Tris-Cl,48mM glycine,20% methanol for 1h in a Trans-Blot semidry electroblotter (Bio-Rad). Blots were blocked overnight with 5% skim milk and incubated with primary antibodies in PBS containing 1% bovine serum albumin ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then transferred onto low-fluorescence 0.45 μm PVDF membranes with transfer buffer (10× Tris/glycine with 10% methanol) (Bio-Rad) for 10 min using the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
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bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
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bioRxiv - Genetics 2019Quote: ... Gels were transferred in buffer containing 20% methanol and 0.03% SDS for 2 hours at 4C at 28V onto 0.2μM nitrocellulose membranes (Bio-Rad, cat #1620097). Membranes were blocked in tris-buffered saline and Tween 20 (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... the gels were wet transferred at 400 mA for 90 min to methanol-activated 0.2 µm pore size PVDF membrane (Bio-Rad). Blots were blocked in 5% nonfat milk in Tris-buffered saline (0.5 mM Tris-HCl [pH 7.5] and 1.5 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Samples were transferred onto a polyvinylidene difluoride membrane activated with methanol using the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were probed with mouse StrepMAB-Classic antibody diluted 1:3000 in TBS-T (25 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 μg protein per sample were loaded onto SDS-page gels for protein separation and then transferred onto nitrocellulose or methanol-activated PVDF membranes via full-wet transfer assay (BioRad) or semi-wet transfer ...
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). After transfer the membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 for 1 hour at room temperature ...