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Citations for Bio-Rad :
101 - 150 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the solution was heated and slowly cooled from 80°C to 4°C (ramp rate of -1°C per 5 s) in a standard thermocycler (Bio-Rad CFX96 Real-Time System) prior to each experiment.
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bioRxiv - Microbiology 2021Quote: ... Bradford Protein Assay Reagents 1 and 2 and Precision Plus Protein All Blue Prestained Protein Standard were from Bio-Rad. The anti His-tag mouse antibody was from GenScript (cat no ...
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-capsid/p24 antibodies were developed following incubation with horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (BioRad; product code 1706515) or IRDye 800CW conjugated goat anti-rabbit IgG antibody (LI-COR ...
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bioRxiv - Pathology 2019Quote: ... Protein concentration in thawed samples (stored at –80°C) was measured using the Bradford method (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... 20 to 40μg of protein were boiled at 95°C for 5 minutes in Laemmli buffer (Biorad), separated on a SDS-PAGE gel and then electrotransfered (100V ...
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bioRxiv - Neuroscience 2019Quote: ... The blotting of the proteins was done at 4 °C overnight at 40 V (PowerPac, Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... Aliquots of protein were boiled at 95°C in 2X Lamelli buffer (Bio-Rad, Hercules, CA, USA) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... All steps were done at 4 °C and concentrations of all proteins were determined by Bio-Rad Protein Assay Dye Reagent Concentrate.
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bioRxiv - Cancer Biology 2023Quote: ... Bound proteins were then eluted through boiling at 100°C in Laemmli sample buffer (BIORAD, CA, USA). The bound proteins were eluted at 37°C for 10 min.
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bioRxiv - Immunology 2024Quote: ... 25µg of protein were denatured at 95°C for 5min in Laemmli Sample Buffer (161747, Bio-Rad) with DTT 0.1M ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell debris was pelleted by microcentrifugation at 4°C and protein levels quantified using Bradford Assays (BioRad) as previously described (38) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Protein estimations were performed using the Bio-Rad microtiter Bradford protein assay (California, USA) according to the instructions provided (Bio-Rad version: Lit 33 Rev C) and measurements were made at 595 nm using a SpectraMax Plus ...
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bioRxiv - Neuroscience 2019Quote: ... Protein sample were diluted 1:1 with 2X SDS Laemmli buffer (Biorad) and incubated for 30 mins at 37°C ...
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Antiviral roles of interferon regulatory factor (IRF)-1, 3 and 7 against human coronavirus infectionbioRxiv - Microbiology 2022Quote: ... human IFN-γ and IFN-λ 1 were obtained from Bio-Rad, BD Pharmingen and R&D Systems respectively ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Genetics 2020Quote: ... and 1 mM EDTA) and protein concentrations were measured using the Protein Assay Kit (Bio-Rad). LPAAT activity was measured by incubating membrane fractions (containing 0.01 μg of protein ...
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bioRxiv - Neuroscience 2022Quote: ... protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) in a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201) and a Tecan Infinite F200 PRO spectrophotometer ...
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bioRxiv - Neuroscience 2021Quote: ... 10-15 μg of protein was boiled at 95°C for 5 min in Laemmli buffer (Bio-Rad), separated on a 4–12 % SDS/PAGE gel and then transferred using the Transblot®-Turbo™ Transfer system (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... After 30 min centrifugation at 4 °C protein concentration of the lysate was determined by Bradford assay (Biorad). In parallel ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were boiled for 5 minutes at 95°C in 4x Laemmli Sample Buffer (Bio-Rad, 1610747) and run on SDS-acrylamide gels ...
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bioRxiv - Genomics 2023Quote: ... proteins were transferred (80 V; 2 h; 4°C) onto a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Membranes were air-dried ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were denatured for 5 min at 95 °C and cooled to RT before acrylamide (#1610140, Bio-Rad) was added to a final concentration of 4% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-cytochrome c (AHP2302, Bio-Rad Laboratories, 1:500), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples in 1× Laemmli sample buffer (Bio-Rad) were loaded and separated by 10% SDS-PAGE gels and then transferred to Immobilon-P membranes (Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Cancer Biology 2022Quote: ... 95°C for 2 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min (BioRad). The following primers from Integrated DNA Technologies (IDT ...
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bioRxiv - Systems Biology 2023Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). For gene expression assays ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Precision Plus Protein™ Western C™ Blotting standards and Precision Protein™ Streptactin-HRP conjugate were used to estimate molecular weight (BioRad, cat# 1610380 and cat#1610376). Relative quantification was performed using ImageJ software (version 1.53p (22) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant contained 2.5 mg proteins/ 1 ml (determined using Bio-Rad Protein Assay, Cat. No. 5000006). This protein fraction was used for co-immunoprecipitation (input) ...
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bioRxiv - Bioengineering 2021Quote: ... and probed overnight at 4°C with IRDye 680 Streptavidin (LICOR) diluted 1:3000 in 1:1 TBST:Blocking Buffer (BioRad). Membranes were imaged using an Azure Biosystems c600 ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...